68results about How to "Conducive to in-depth research" patented technology

The present invention provides the combining method, structure and preparation process of electrochemical supermicro electrode. The electrochemical supermicro electrode is a two-electrode system with one supermicro electrode and one aligning / reference electrode combined together and separated with nanometer level thickness insulating layer. After diffusion layers on these two electrodes are coincided, the electrochemically reversible material particle may be oxidized or reduced on one electrode and reducing or oxidizing regenerated in the other electrode before being re-diffused to original electrode, so as to amplify current signal output by the cell detecting electrode. The present invention has obviously reduced solution resistance, high scanning speed and detection sensitivity, minimized damage to the researched system and other advantages.

The invention discloses a preparation method for stannic oxide / carbon composite hollow balls. Solder balls are added into an aqueous solution of glucose for ultrasonic dispersion, and then is transferred to a hydro-thermal reaction kettle; then the temperature is raised to 160-200 DEG C, and preserved for 30-720 minutes; after the reaction, centrifugal separation and drying are implemented; and then thermal treatment is implemented under nitrogen atmosphere to obtain a final product. In the preparation method, the solder balls are served as sacrificial templates, which do not need to be removed after the reaction; therefore, mass production can be realized; stannic oxide / carbon composite hollow balls are synthesized through simple one-step hydro-thermal reaction, which further simplifies processing steps, and reduces the cost; moreover, the proportions of stannic oxide to carbon are controllable, which is beneficial for subsequently intensive study on the performances of lithium ion batteries manufactured by stannic oxide / carbon composite hollow balls.

The invention belongs to microbiological animal cell system fiels, which in detail relates to a pancreatic cancer hepatic metastases clone SW1990HM with hepatic metastases potence. The clone possesses special transition phenotype, and if inoculated to mouse spleen, it will result to 100% hepatic metastases, and it is the first human pancreatic cancer clone with hepatic metastases potence in domestic. The genetic backgroud of said clone strain is the same to that of origianl cell, and it is characterized by strong invasiveness, high transmission efficiency and large transmission range. The invention provides proper experimental platform for further researching, preventing and treating pancreatic cancer hepatic metastases.

The invention discloses a preparation method of an iron trioxide / carbon yolk-eggshell nano-composite structure. The preparation method includes taking iron trioxide nano-particles as centers, controlling thickness of coated silica through controlling quantity of ethyl orthosilicate, coating a layer of carbon outside silica through a thermal decomposition method, and obtaining the iron trioxide / carbon yolk-eggshell nano-composite structure by removing the silica in the middle layer. With the preparation method, the iron trioxide / carbon yolk-eggshell nano-composite structure is composited through simple coating processes, production cost is reduced and mass production can be realized; and besides, the hollow iron trioxide / carbon yolk-eggshell nano-composite structure is beneficial for improving performance of lithium ion battery negative electrode materials.

The invention discloses a method for screening bio-control bacteria of cucumber fusarium wilt, and provides a method for identifying the bio-control nature of to-be-identified bacteria to pathogenic bacteria of the cucumber fusarium wilt including experimental treatment. The experiment treatment sequentially includes following steps of 1), accelerating sprouting of sterilized cucumber seeds to obtain the sprouting seeds; 2), subjecting the sprouting seeds to soaking in suspension liquid of the to-be-identified bacteria prior to cultivating the sprouting seeds till radicle length reaches by 1.5-2.0cm; 3), inoculating cucumber seedlings to suspension liquid of the pathogenic bateria of the cucumber fusarium wilt; 4), transferring the cucumber seedlings to MS solid media to cultivate, and identifying the bio-control nature of the to-be-identified bacteria to the patheogenic bacteria of the cucumber fusarium wilt by surveying disease indexes and / or observing disease phenotypes. The method is applicable to screening the bio-control bacteria of the cucumber fusarium wilt. In the method, the cucumber seedlings used as samples can truly reflect disease prevention effect of the bio-control bacteria, and can screen bacterial strains of various bio-control mechanisms.

The invention relates to a rhodiola rosea and a rhodioside application for inducing stem cells to directionally differentiate into hepatocytes, in particular to a compound inducer of the rhodioside and cell growth factor FGF-4 used for inducing mesenchymal stem cells to directionally differentiate into hepatocytes. The rhodiola rosea develops the new application of rhodiola, which induces mesenchymal stem cells and other stem cells to directionally differentiate into hepatocytes in vitro, and provides the effect of the rhodiola rosea and the rhodioside on inducing the adult stem cells to directionally differentiate into hepatocytes, which is beneficial to further research of the in vivo stem cells transplantation for treating acute or chronic hepatic injury and middle-advanced liver disease field, and provides the basic for new drug development.

The invention belongs to the field of mineral and rock materials and in particular relates to a new method for measuring a heat conductivity coefficient of a micro (gram-scale) mineral and rock powder sample in a vacuum environment under variable temperature and variable pressure conditions. The needed volume of the micro powder sample is in a range from 1.0cm<3> to 20.0cm<3> during test. The method is performed under vacuum conditions and comprises the following test steps: (1) performing sample pretreatment, namely preparing the sample into a powder sample with the particle size of not more than 1mm according to own needs, ensuring that the sample is uniformly mixed, and drying the sample before measurement; (2) mounting the sample, namely selecting sample cells of different sizes and volumes according to the sample quantity, putting the pretreated sample and a test probe into the sample cells for fixing, and finally arranging the sample cells in a vacuum cavity of a freezer dryer; and (3) testing the sample, namely vacuumizing the vacuum cavity of the freezer dryer, adjusting the pressure to be in a range from 1Pa to 1.013*10<5>Pa, and adjusting the temperature in the cavity, wherein the adjustable temperature ranges from 190 DEG C below zero to 200 DEG C. After the pressure and temperature reach the specified values, the heat conductivity coefficient value of the sample under specified testing conditions is obtained through operation of thermal conductance meter related software.

The invention discloses an astragalus mongholicus formulation for veterinary use and a preparation method thereof, and belongs to the technical of extraction and application of effective components of plant traditional Chinese medicine. The medicine comprises two active ingredients including astragalus polysaccharide and astragalus polypeptide, and the form of the medicine is injection, oral liquid or powder. The preparation method includes comprehensively utilizing an enzyme hydrolysis extraction technology to effectively extract the high-activity astragalus polypeptide and the high-content high-purity astragalus polysaccharide from raw materials of astragalus mongholicus and effectively preparing the astragalus mongholicus formulation. The technical problems of comprehensive extraction and preparation are solved, further systematic and deep research on the astragalus polysaccharide and the astragalus polypeptide is facilitated, yield and purity are greatly increased, resource waste is reduced, cost is lowered, and industrialized production is facilitated.

The invention relates to a preparation method of one-dimensional Fe3O4 nanoneedle material provided with a modulated structure. The preparation method of the one-dimensional Fe3O4 nanoneedle material provided with the modulated structured is characterized in that the preparation method comprises the steps that firstly, ferrous chloride, boric acid, saccharin and sodium fluoride are dissolved into distilled water, and then the pH value is adjusted to 2.0 by dripping weak sulphuric acid or sodium hydroxide solution to be prepared into electrolyzed solution to be positioned in a hull cell; 380 g of ferrous chloride, 20 g of boric acid, 5 g of saccharin and 2.5 g of sodium fluoride are prepared in the electrolyzed solution per liter; secondly, the pulse galvanic depositing technology is adopted, and the pulse galvanic depositing parameter with output pulse frequency f of 50 to 500 Hz, duty factor r of 5 to 50 percent, positive pulse operating current of 0.3 to 0.5 A and negative operating current of 0.1 to 0.3A is adopted; a conductive metal base plate is positioned in the hull cell, the pulse galvanic depositing is lasted for 3 to 20 minutes, and a layer of nanocrystalline Fe thin film is electrically deposited on the surface of the conductive metal base plate; thirdly, the nanocrystalline Fe thin film is heated for 0.5 to 3 hours at 900 DEG C, to obtain the one-dimensional Fe3O4 nanoneedle material provided with t modulated structure. The method has the advantages that the process is simple, the controllable degree is high, the preparation method conforms to the environment requirement, and the cost is low.

The invention discloses a preparation method for a polyclonal antibody to salmonella effect protein SopB. The invention aims to provide the preparation method for the rabbit-derived polyclonal antibody with good specificity, high sensitivity and capability of specifically binding to the endogenous salmonella effect protein SopB secreted in the process of salmonella infection of a host cell. A protein sequence as represented by SEQ ID No. 2 is used as immunogen, wherein the protein sequence consists of 139 amino acids located between position 29 and position 168, counted from terminal N, of a full-length amino acid sequence as represented by SEQ ID No. 1 of the salmonella effect protein SopB, and a conventional preparation method for a polyclonal antibody is used for preparation of antiserum; then the antiserum is purified so as to obtain the polyclonal antibody to the salmonella effect protein SopB. According to the invention, the polyclonal antibody to the salmonella effect protein SopB is prepared by using the method, and the polyclonal antibody is beneficial for immunoblotting analysis and immunofluorescent staining laser confocal microscopic observation of the salmonella effect protein SopB and for in-depth research on the infection mechanism of enteropathogens.

The invention discloses a quantitative defect characterization and bursting strength prediction method for a defective geomembrane. According to the method, geomembrane defects are divided into porousdefects, crack-shaped defects and damage scratch defects, the porous defects are characterized by an equivalent aperture, the crack-shaped defects are characterized by an equivalent defect length, and the damage scratch defects are characterized by using a damage degree. Meanwhile, the invention provides a prediction method for the bursting strength of the geomembrane containing the defects. Theprediction method comprises the following steps: preparing a defect geomembrane sample; carrying out bursting test of geomembranes with different defect; establishing a geomembrane bursting strength prediction formula containing different defects; and predicting the bursting strength of the geomembrane containing the defects on the construction site. According to the methods, a set of complete geomembrane defect classification criteria is determined; quantitative characterization of the geomembrane defect damage degree is achieved, a calculation formula of the bursting strength of the geomembrane with the defects is established, the method is used for predicting the bursting strength of the geomembrane with different defects on a complex construction site, and the methods have important scientific research significance and engineering application value for guiding construction of the geomembrane.

The invention provides new application of danshinolic acid C, and particularly, the danshinolic acid C can be used for preparing a drug for protecting the ischemic brain tissue damage. The danshinolicacid C has a good effect of resisting the ischemic brain tissue damage, the effect is obvious, and the danshinolic acid C has development and utilization values.

The invention relates to a control method of a fuel injection simulation system capable of regulating pressure and temperature. The fuel injection simulation system realizes a high-pressure fuel supply mode and a low-pressure fuel supply mode for supplying fuel through a high-pressure fuel pump and a low-pressure fuel pump and utilizes silicone oil to control temperature. Thus, the fuel injection simulation system can simulate the character of fuel injection under various conditions and particularly can simulate the character of fuel injection under extreme conditions.

The invention relates to a rhodiola rosea and a rhodioside application for inducing stem cells to directionally differentiate into hepatocytes, in particular to a compound inducer of the rhodioside and cell growth factor FGF-4 used for inducing mesenchymal stem cells to directionally differentiate into hepatocytes. The rhodiola rosea develops the new application of rhodiola, which induces mesenchymal stem cells and other stem cells to directionally differentiate into hepatocytes in vitro, and provides the effect of the rhodiola rosea and the rhodioside on inducing the adult stem cells to directionally differentiate into hepatocytes, which is beneficial to further research of the in vivo stem cells transplantation for treating acute or chronic hepatic injury and middle-advanced liver disease field, and provides the basic for new drug development.

The invention discloses a jellyfish acetabulum culture device and a use method thereof. The jellyfish acetabulum culture device structurally comprises a culture bottle and an electric bottle rolling machine. The culture bottle comprises a cylindrical bottle body with openings in two ends, and side covers capable of being detachably connected with the two ends of the bottle body. A hole is formed in the middle of each side cover. Hemispherical pits are evenly formed in the bottle body. In the specific experiment process, acetabulum can float along with the low-speed effective water flow in the culture bottle, and the cylindrical bottle body is free of dead corners and inflation pipes, so individual damage is effectively avoided; meanwhile, a water body does not produce bubbles, so it can be effectively avoided that when bubbles enter a gastral cavity, acetabulum floats up, and the death rate is decreased. The survival rate of jellyfish acetabulum is practically increased, and the accuracy of indoor research experiments is ensured. The deep research on disaster jellyfish and economic jellyfish is facilitated, and the jellyfish acetabulum culture device is widely applied in related research fields such as fishery resource utilization and ecological restoration.

The invention provides an annual slide preparation method of plum blossom chromosome. According to the method, the following meristems are selected as materials for preparing the slide according to time: 1) spring: new stem tips of branches in early spring; 2) early summer: immature embryos of plum blossom and growth points obtained from tissue culture of immature embryos; 3) summer and autumn: top growth points of annual seedlings of plum blossom; and 4) winter: stem tips obtained from hydroponics of dormant branches of plum blossom. The above materials for preparing the slide are treated with chromosome slide preparation, which comprises successive steps of low temperature fixing, enzyme and dyeing tabletting, to obtain a clear chromosome image with good dispersion. The method provided by the invention solves problems of short material collection cycle and time restriction of chromosome slide preparation in the study course of plum blossom chromosome.

The invention discloses a plant light system I polyclonal antibody and a preparation method thereof. The antigenic epitope of the light system I related protein and the synthesized corresponding KLH coupled polypeptide are designed, the series of coupled polypeptides serve as immunogens to immunize animals to obtain corresponding antibodies, the antibodies are used as detection reagents for detecting the plant light system I related protein through an ELISA method, full detection and accurate quantitative detection of the light system I related protein are achieved, and deep research on the optical system I is facilitated.

The invention discloses an S-ZVI magnetic environment restoration material as well as a preparation method and application thereof. Ferric ions are reduced into zero-valent iron by using a reducing agent (sodium borohydride and potassium borohydride), then a sulfur source (such as sodium thiosulfate) is added to form a layer of ferrous sulfide on the surface of the zero-valent iron, and the S-ZVI material is prepared. After the S-ZVI material is modified by the sulfur source, the S-ZVI material overcomes the problems that zero-valent iron is easy to agglomerate and passivate, and the electron transfer rate and the reaction activity of the zero-valent iron are improved. The environmental remediation material prepared by the preparation method is low in cost, the preparation method is simple and convenient, the negative influence on the environment is small, meanwhile, the material prepared by the method has the advantage in removing heavy metal chromium, and the material is good in stability and convenient to recycle.

The invention discloses a plant photosystem II polyclonal antibody and a preparation method thereof. Epitopes of related proteins in the photosystem II and synthesized corresponding KLH coupling polypeptides are designed, the series of the coupling polypeptides are adopted as an immunogen to immunize animals to obtain a corresponding antibody, and a detection reagent for detecting the related proteins in the plant photosystem II by a western blot method realizes full detection, accurate quantitative detection of the related proteins in the photosystem II and in-depth research on the photosystem II is facilitated.

The invention discloses a modular centrifugal pendulum-type dynamic vibration absorber experiment table, and the experiment table comprises an excitation source, a adapter module and at least one free combination module, which are connected in sequence. The excitation source comprises an excitation source base; a rotating seat which is movably arranged on the excitation source base in an angle-adjustable mode; and a motor which is fixedly arranged on the rotating seat through a motor support. The adapter module comprises an adapter seat; a bracket which is fixed on the adapter seat through a bolt; a sliding bearing which is fixed at the upper end of the bracket; and an adapter shaft which is in sliding fit with the sliding bearing, wherein one end of the adapter shaft is in driving connection with an output shaft of the motor through a cross shaft universal coupling. The free combination module comprises a combination base, two supports and a spring-quality system with the adjustable inherent frequency. The experiment table is simple in structure, small in occupied space, easy and convenient to disassemble and assemble, convenient to transform and maintain and beneficial to deep research on the working effect of a centrifugal pendulum-type dynamic vibration absorber and the like.

The invention relates to a method for preparing a counterfeit antiferroelectric film, belonging to the technical field of micro-electronics. In the preparation method, stably distributed doping ions are formed in a ferroelectric film by injecting and doping a certain dose of hydrogen ions with certain energy, and the overturning characteristic of a ferroelectric film domain is changed through a charge compensation mode. The preparation method can realize the antiferroelectric characteristic of the ferroelectric film in the ferroelectric film with any component proportion and can be applied to the field of storage of high-density charges. The invention not only provides a new preparation method of the counterfeit antiferroelectric film, but also is beneficial to further intensive study on the overturning physical mechanism of the electric domain of the ferroelectric film, and has great academic significance.

The invention discloses a test model for determining a microscopic damping parameter of miscellaneous fill. The test model for determining the microscopic damping parameter of the miscellaneous fill comprises a base, a slide groove device, a supporting device, a control device, an observation unit and a linking unit, wherein the observation unit is used for recording and simulating an inclined trajectory of a landslide body; and the linking unit is used for realizing synchronous operation of the observation unit and the control device. The slide groove device comprises three-section type slopeslide grooves end to end, wherein a middle section slope can retract or extend; the control device comprises a control platform, a vertical support, an oblique support, a control platform lifting adjusting device, a driving part and a starting device, wherein the driving part and the starting device are arranged on the control platform; the bottom of a starting device executing part is arranged in the slope slide groove and in sealing fit with the slope slide groove; the vertical support is linked with an automatic angular instrument at the bottom of the slide groove to mutually control an inclined angle of the slide groove; and the oblique support controls the length of a middle section slide groove. The test model for determining the microscopic damping parameter of the miscellaneous fill, disclosed by the invention, has the advantages that included angles between the three sections of slide grooves can be adjusted arbitrarily; through the extension of the middle section, the path of the slope is more diversified, and the application is wider. Therefore, the microscopic damping parameter of the miscellaneous fill is more accurately obtained.

The invention discloses a separation and verification method of different electron-acceptor phosphorus-accumulating bacteria in activated sludge, and belongs to the technical field of biological sewage treatment. The separation and verification method can separate the DNA of different electron acceptor phosphorus-accumulating bacteria in the activated sludge system of a sewage treatment plant, and analyze the characteristics of florae on the gene level by adopting a molecular biological method. The separation and verification method comprises the following steps: adopting 13C-sodium acetate as the carbon source of an experimental group and 12C-sodium acetate as the carbon source of a control group, conducting anaerobic phosphorus release for 2 hours, respectively using oxygen, nitrate and nitrite as electron acceptors, conducting aerobic or anoxic phosphorus uptake culture for 3 hours, conducting culture for 8 cycles totally, conducting ultrahigh-speed centrifugal separation and purification to obtain DNA samples with different density levels, and determining and analyzing the flora structures of the phosphorus-accumulating bacteria when the electron acceptors are different by a molecular biological method. The invention provides the method for analyzing the electron acceptors required by different phosphorus-accumulating bacteria, and provides a theoretical support for the denitrification-strengthened dephosphorization process of the sewage treatment plant practically.

The invention discloses a soil-rock mixture cyclic shear seepage test system characterized by comprising a horizontal loading system, a vertical loading system, shear boxes, a seepage water pressure loading system and a data acquisition and processing system; the horizontal loading system comprises a left horizontal loading system and a right horizontal loading system; the vertical loading system is arranged above an upper shear box and comprises a rolling shaft row, a force transmission plate, a vertical displacement sensor, a vertical load sensor and a vertical jack, and the shear box comprises a lower shear box and the upper shear box located above the lower shear box; the permeation water pressure loading system comprises a water pressure pump, a measuring cup, a water inlet pipeline and a water outlet pipeline; and the data acquisition and processing system comprises a data acquisition device and a data processing device. The invention further discloses a corresponding test method. Through the left horizontal loading system and the right horizontal loading system, cyclic loading of a soil-rock mixture can be achieved, various types of tests can be achieved, and guidance is provided for engineering practice.

The invention discloses a preparation method for a polyclonal antibody to salmonella effect protein SopB. The invention aims to provide the preparation method for the rabbit-derived polyclonal antibody with good specificity, high sensitivity and capability of specifically binding to the endogenous salmonella effect protein SopB secreted in the process of salmonella infection of a host cell. A protein sequence as represented by SEQ ID No. 2 is used as immunogen, wherein the protein sequence consists of 139 amino acids located between position 29 and position 168, counted from terminal N, of a full-length amino acid sequence as represented by SEQ ID No. 1 of the salmonella effect protein SopB, and a conventional preparation method for a polyclonal antibody is used for preparation of antiserum; then the antiserum is purified so as to obtain the polyclonal antibody to the salmonella effect protein SopB. According to the invention, the polyclonal antibody to the salmonella effect protein SopB is prepared by using the method, and the polyclonal antibody is beneficial for immunoblotting analysis and immunofluorescent staining laser confocal microscopic observation of the salmonella effect protein SopB and for in-depth research on the infection mechanism of enteropathogens.

The invention discloses an experimental metal / water reaction hydrogen production device and a metal / water reaction hydrogen production collection method and belongs to the technical field of experimental hydrogen production devices. The experimental device comprises a solution storing mechanism, a reacting mechanism and a hydrogen volume detecting mechanism, wherein the solution storing mechanism is communicated with the reacting mechanism, and the reacting mechanism is communicated with the hydrogen volume detecting mechanism. A thermostatic water bath tank is arranged above a magnetic stirrer, a three-opening beaker flask is arranged in the thermostatic water bath tank and provided with three openings including a solution opening, a metal powder opening and a hydrogen opening, the solution opening is communicated with the solution storing mechanism, the metal powder opening is sealed by a sealing plug, and the hydrogen opening is communicated with the hydrogen volume detecting mechanism. A fixing support is fixed above the magnetic stirrer, fixedly connected with the three-opening beaker flask and used for fixing the three-opening beaker flask during reaction. The experimental metal / water reaction hydrogen production device and the metal / water reaction hydrogen production collection method have the advantages that hydrogen collection and automatic recording of produced hydrogen volume are simplified.

The invention provides a universal machine learning data analysis platform. The universal machine learning data analysis platform comprises an interface module, a data storage module, a preprocessing module, a feature extraction module, a feature conversion module, an algorithm module and a selection optimization module, wherein the feature extraction module extracts feature parameters from to-be-analyzed data according to the feature parameters set by a user; the feature conversion module is used for converting features set by the user to be in a representation form required by the user; the algorithm module comprises a plurality of algorithm models for the user to select and build, and the user builds at least one group of models; the selection optimization module selects out an optimal model and an optimal parameter from the built models and then stores the optimal model; and data generated by the modules is stored in the data storage module. According to the platform, the user can freely combine and use the modules and the algorithm models, and also can build a complex model and quickly and iteratively develop a novel analytic model, so that the working efficiency is greatly improved.

The invention provides a five-eye tilt camera device and an unmanned aerial vehicle. The five-eye tilt camera device comprises a mounting mechanism; an upper shell, a middle shell and a lower shell which are sequentially connected, wherein the upper shell is connected with a mounting machine, the lower shell is provided with a circular-truncated-cone-shaped groove, and an annular notch is formed in the side wall of the circular-truncated-cone-shaped groove; cameras which comprise a main camera and four inclined cameras, wherein the main camera is located in the center of the circular-truncated-cone-shaped groove, the four inclined cameras are arranged on the side wall of the circular-truncated-cone-shaped groove, and a semi-arc-shaped camera shell is arranged on the cameras; gears arrangedin the lower shell, wherein one gear is arranged under each inclined camera, the gears under any two adjacent inclined cameras are meshed, and the gears under the other two inclined cameras are meshed; and telescopic rods, wherein each inclined camera is provided with a pair of telescopic rods, the fixed ends of the telescopic rods are arranged on the gears, and the telescopic ends of the telescopic rods are connected with the camera shell. The manufacturing period and the manufacturing cost of the image can be reduced, and researchers can conduct deep research.