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68results about How to "Conducive to in-depth research" patented technology

New method for measuring heat conductivity coefficient of micro powder sample under variable temperature and variable pressure conditions

The invention belongs to the field of mineral and rock materials and in particular relates to a new method for measuring a heat conductivity coefficient of a micro (gram-scale) mineral and rock powder sample in a vacuum environment under variable temperature and variable pressure conditions. The needed volume of the micro powder sample is in a range from 1.0cm<3> to 20.0cm<3> during test. The method is performed under vacuum conditions and comprises the following test steps: (1) performing sample pretreatment, namely preparing the sample into a powder sample with the particle size of not more than 1mm according to own needs, ensuring that the sample is uniformly mixed, and drying the sample before measurement; (2) mounting the sample, namely selecting sample cells of different sizes and volumes according to the sample quantity, putting the pretreated sample and a test probe into the sample cells for fixing, and finally arranging the sample cells in a vacuum cavity of a freezer dryer; and (3) testing the sample, namely vacuumizing the vacuum cavity of the freezer dryer, adjusting the pressure to be in a range from 1Pa to 1.013*10<5>Pa, and adjusting the temperature in the cavity, wherein the adjustable temperature ranges from 190 DEG C below zero to 200 DEG C. After the pressure and temperature reach the specified values, the heat conductivity coefficient value of the sample under specified testing conditions is obtained through operation of thermal conductance meter related software.
Owner:INST OF GEOCHEM CHINESE ACADEMY OF SCI

Preparation of one-dimensional Fe3O4 nano-needle material having modulated structure

The invention relates to a preparation method of one-dimensional Fe3O4 nanoneedle material provided with a modulated structure. The preparation method of the one-dimensional Fe3O4 nanoneedle material provided with the modulated structured is characterized in that the preparation method comprises the steps that firstly, ferrous chloride, boric acid, saccharin and sodium fluoride are dissolved into distilled water, and then the pH value is adjusted to 2.0 by dripping weak sulphuric acid or sodium hydroxide solution to be prepared into electrolyzed solution to be positioned in a hull cell; 380 g of ferrous chloride, 20 g of boric acid, 5 g of saccharin and 2.5 g of sodium fluoride are prepared in the electrolyzed solution per liter; secondly, the pulse galvanic depositing technology is adopted, and the pulse galvanic depositing parameter with output pulse frequency f of 50 to 500 Hz, duty factor r of 5 to 50 percent, positive pulse operating current of 0.3 to 0.5 A and negative operating current of 0.1 to 0.3A is adopted; a conductive metal base plate is positioned in the hull cell, the pulse galvanic depositing is lasted for 3 to 20 minutes, and a layer of nanocrystalline Fe thin film is electrically deposited on the surface of the conductive metal base plate; thirdly, the nanocrystalline Fe thin film is heated for 0.5 to 3 hours at 900 DEG C, to obtain the one-dimensional Fe3O4 nanoneedle material provided with t modulated structure. The method has the advantages that the process is simple, the controllable degree is high, the preparation method conforms to the environment requirement, and the cost is low.
Owner:WUHAN UNIV OF TECH

Preparation method for polyclonal antibody to salmonella effect protein SopB

InactiveCN103204936AGood for western blot analysisFavorable for immunohistochemical analysisHydrolasesSerum immunoglobulinsStainingMicroscopic observation
The invention discloses a preparation method for a polyclonal antibody to salmonella effect protein SopB. The invention aims to provide the preparation method for the rabbit-derived polyclonal antibody with good specificity, high sensitivity and capability of specifically binding to the endogenous salmonella effect protein SopB secreted in the process of salmonella infection of a host cell. A protein sequence as represented by SEQ ID No. 2 is used as immunogen, wherein the protein sequence consists of 139 amino acids located between position 29 and position 168, counted from terminal N, of a full-length amino acid sequence as represented by SEQ ID No. 1 of the salmonella effect protein SopB, and a conventional preparation method for a polyclonal antibody is used for preparation of antiserum; then the antiserum is purified so as to obtain the polyclonal antibody to the salmonella effect protein SopB. According to the invention, the polyclonal antibody to the salmonella effect protein SopB is prepared by using the method, and the polyclonal antibody is beneficial for immunoblotting analysis and immunofluorescent staining laser confocal microscopic observation of the salmonella effect protein SopB and for in-depth research on the infection mechanism of enteropathogens.
Owner:TIANJIN UNIV OF COMMERCE

Quantitative defect characterization and bursting strength prediction methods for defective geomembrane

The invention discloses a quantitative defect characterization and bursting strength prediction method for a defective geomembrane. According to the method, geomembrane defects are divided into porousdefects, crack-shaped defects and damage scratch defects, the porous defects are characterized by an equivalent aperture, the crack-shaped defects are characterized by an equivalent defect length, and the damage scratch defects are characterized by using a damage degree. Meanwhile, the invention provides a prediction method for the bursting strength of the geomembrane containing the defects. Theprediction method comprises the following steps: preparing a defect geomembrane sample; carrying out bursting test of geomembranes with different defect; establishing a geomembrane bursting strength prediction formula containing different defects; and predicting the bursting strength of the geomembrane containing the defects on the construction site. According to the methods, a set of complete geomembrane defect classification criteria is determined; quantitative characterization of the geomembrane defect damage degree is achieved, a calculation formula of the bursting strength of the geomembrane with the defects is established, the method is used for predicting the bursting strength of the geomembrane with different defects on a complex construction site, and the methods have important scientific research significance and engineering application value for guiding construction of the geomembrane.
Owner:HOHAI UNIV

Jellyfish acetabulum culture device and use method thereof

The invention discloses a jellyfish acetabulum culture device and a use method thereof. The jellyfish acetabulum culture device structurally comprises a culture bottle and an electric bottle rolling machine. The culture bottle comprises a cylindrical bottle body with openings in two ends, and side covers capable of being detachably connected with the two ends of the bottle body. A hole is formed in the middle of each side cover. Hemispherical pits are evenly formed in the bottle body. In the specific experiment process, acetabulum can float along with the low-speed effective water flow in the culture bottle, and the cylindrical bottle body is free of dead corners and inflation pipes, so individual damage is effectively avoided; meanwhile, a water body does not produce bubbles, so it can be effectively avoided that when bubbles enter a gastral cavity, acetabulum floats up, and the death rate is decreased. The survival rate of jellyfish acetabulum is practically increased, and the accuracy of indoor research experiments is ensured. The deep research on disaster jellyfish and economic jellyfish is facilitated, and the jellyfish acetabulum culture device is widely applied in related research fields such as fishery resource utilization and ecological restoration.
Owner:LIAONING OCEAN & FISHERIES SCI RES INST

Modular centrifugal pendulum-type dynamic vibration absorber experiment table

The invention discloses a modular centrifugal pendulum-type dynamic vibration absorber experiment table, and the experiment table comprises an excitation source, a adapter module and at least one free combination module, which are connected in sequence. The excitation source comprises an excitation source base; a rotating seat which is movably arranged on the excitation source base in an angle-adjustable mode; and a motor which is fixedly arranged on the rotating seat through a motor support. The adapter module comprises an adapter seat; a bracket which is fixed on the adapter seat through a bolt; a sliding bearing which is fixed at the upper end of the bracket; and an adapter shaft which is in sliding fit with the sliding bearing, wherein one end of the adapter shaft is in driving connection with an output shaft of the motor through a cross shaft universal coupling. The free combination module comprises a combination base, two supports and a spring-quality system with the adjustable inherent frequency. The experiment table is simple in structure, small in occupied space, easy and convenient to disassemble and assemble, convenient to transform and maintain and beneficial to deep research on the working effect of a centrifugal pendulum-type dynamic vibration absorber and the like.
Owner:SOUTH CHINA UNIV OF TECH

Test model for determining microscopic damping parameter of miscellaneous fill

The invention discloses a test model for determining a microscopic damping parameter of miscellaneous fill. The test model for determining the microscopic damping parameter of the miscellaneous fill comprises a base, a slide groove device, a supporting device, a control device, an observation unit and a linking unit, wherein the observation unit is used for recording and simulating an inclined trajectory of a landslide body; and the linking unit is used for realizing synchronous operation of the observation unit and the control device. The slide groove device comprises three-section type slopeslide grooves end to end, wherein a middle section slope can retract or extend; the control device comprises a control platform, a vertical support, an oblique support, a control platform lifting adjusting device, a driving part and a starting device, wherein the driving part and the starting device are arranged on the control platform; the bottom of a starting device executing part is arranged in the slope slide groove and in sealing fit with the slope slide groove; the vertical support is linked with an automatic angular instrument at the bottom of the slide groove to mutually control an inclined angle of the slide groove; and the oblique support controls the length of a middle section slide groove. The test model for determining the microscopic damping parameter of the miscellaneous fill, disclosed by the invention, has the advantages that included angles between the three sections of slide grooves can be adjusted arbitrarily; through the extension of the middle section, the path of the slope is more diversified, and the application is wider. Therefore, the microscopic damping parameter of the miscellaneous fill is more accurately obtained.
Owner:HOHAI UNIV

Separation and verification method of different electron-acceptor phosphorus-accumulating bacteria in activated sludge

ActiveCN107142306APracticalRich Molecular Biology MethodsMicrobiological testing/measurementChemistryCarbon source
The invention discloses a separation and verification method of different electron-acceptor phosphorus-accumulating bacteria in activated sludge, and belongs to the technical field of biological sewage treatment. The separation and verification method can separate the DNA of different electron acceptor phosphorus-accumulating bacteria in the activated sludge system of a sewage treatment plant, and analyze the characteristics of florae on the gene level by adopting a molecular biological method. The separation and verification method comprises the following steps: adopting 13C-sodium acetate as the carbon source of an experimental group and 12C-sodium acetate as the carbon source of a control group, conducting anaerobic phosphorus release for 2 hours, respectively using oxygen, nitrate and nitrite as electron acceptors, conducting aerobic or anoxic phosphorus uptake culture for 3 hours, conducting culture for 8 cycles totally, conducting ultrahigh-speed centrifugal separation and purification to obtain DNA samples with different density levels, and determining and analyzing the flora structures of the phosphorus-accumulating bacteria when the electron acceptors are different by a molecular biological method. The invention provides the method for analyzing the electron acceptors required by different phosphorus-accumulating bacteria, and provides a theoretical support for the denitrification-strengthened dephosphorization process of the sewage treatment plant practically.
Owner:BEIJING UNIV OF TECH

Soil-rock mixture cyclic shear seepage test system and test method thereof

The invention discloses a soil-rock mixture cyclic shear seepage test system characterized by comprising a horizontal loading system, a vertical loading system, shear boxes, a seepage water pressure loading system and a data acquisition and processing system; the horizontal loading system comprises a left horizontal loading system and a right horizontal loading system; the vertical loading system is arranged above an upper shear box and comprises a rolling shaft row, a force transmission plate, a vertical displacement sensor, a vertical load sensor and a vertical jack, and the shear box comprises a lower shear box and the upper shear box located above the lower shear box; the permeation water pressure loading system comprises a water pressure pump, a measuring cup, a water inlet pipeline and a water outlet pipeline; and the data acquisition and processing system comprises a data acquisition device and a data processing device. The invention further discloses a corresponding test method. Through the left horizontal loading system and the right horizontal loading system, cyclic loading of a soil-rock mixture can be achieved, various types of tests can be achieved, and guidance is provided for engineering practice.
Owner:INST OF ROCK AND SOIL MECHANICS - CHINESE ACAD OF SCI +2

Preparation method for polyclonal antibody to salmonella effect protein SopB

InactiveCN103204936BGood for western blot analysisFavorable for immunohistochemical analysisHydrolasesSerum immunoglobulinsStainingMicroscopic observation
The invention discloses a preparation method for a polyclonal antibody to salmonella effect protein SopB. The invention aims to provide the preparation method for the rabbit-derived polyclonal antibody with good specificity, high sensitivity and capability of specifically binding to the endogenous salmonella effect protein SopB secreted in the process of salmonella infection of a host cell. A protein sequence as represented by SEQ ID No. 2 is used as immunogen, wherein the protein sequence consists of 139 amino acids located between position 29 and position 168, counted from terminal N, of a full-length amino acid sequence as represented by SEQ ID No. 1 of the salmonella effect protein SopB, and a conventional preparation method for a polyclonal antibody is used for preparation of antiserum; then the antiserum is purified so as to obtain the polyclonal antibody to the salmonella effect protein SopB. According to the invention, the polyclonal antibody to the salmonella effect protein SopB is prepared by using the method, and the polyclonal antibody is beneficial for immunoblotting analysis and immunofluorescent staining laser confocal microscopic observation of the salmonella effect protein SopB and for in-depth research on the infection mechanism of enteropathogens.
Owner:TIANJIN UNIV OF COMMERCE

Experimental metal/water reaction hydrogen production device and metal/water reaction hydrogen production collection method

The invention discloses an experimental metal / water reaction hydrogen production device and a metal / water reaction hydrogen production collection method and belongs to the technical field of experimental hydrogen production devices. The experimental device comprises a solution storing mechanism, a reacting mechanism and a hydrogen volume detecting mechanism, wherein the solution storing mechanism is communicated with the reacting mechanism, and the reacting mechanism is communicated with the hydrogen volume detecting mechanism. A thermostatic water bath tank is arranged above a magnetic stirrer, a three-opening beaker flask is arranged in the thermostatic water bath tank and provided with three openings including a solution opening, a metal powder opening and a hydrogen opening, the solution opening is communicated with the solution storing mechanism, the metal powder opening is sealed by a sealing plug, and the hydrogen opening is communicated with the hydrogen volume detecting mechanism. A fixing support is fixed above the magnetic stirrer, fixedly connected with the three-opening beaker flask and used for fixing the three-opening beaker flask during reaction. The experimental metal / water reaction hydrogen production device and the metal / water reaction hydrogen production collection method have the advantages that hydrogen collection and automatic recording of produced hydrogen volume are simplified.
Owner:ANHUI UNIVERSITY OF TECHNOLOGY

Five-eye tilt camera device and unmanned aerial vehicle

The invention provides a five-eye tilt camera device and an unmanned aerial vehicle. The five-eye tilt camera device comprises a mounting mechanism; an upper shell, a middle shell and a lower shell which are sequentially connected, wherein the upper shell is connected with a mounting machine, the lower shell is provided with a circular-truncated-cone-shaped groove, and an annular notch is formed in the side wall of the circular-truncated-cone-shaped groove; cameras which comprise a main camera and four inclined cameras, wherein the main camera is located in the center of the circular-truncated-cone-shaped groove, the four inclined cameras are arranged on the side wall of the circular-truncated-cone-shaped groove, and a semi-arc-shaped camera shell is arranged on the cameras; gears arrangedin the lower shell, wherein one gear is arranged under each inclined camera, the gears under any two adjacent inclined cameras are meshed, and the gears under the other two inclined cameras are meshed; and telescopic rods, wherein each inclined camera is provided with a pair of telescopic rods, the fixed ends of the telescopic rods are arranged on the gears, and the telescopic ends of the telescopic rods are connected with the camera shell. The manufacturing period and the manufacturing cost of the image can be reduced, and researchers can conduct deep research.
Owner:LIAOYANG POWER SUPPLY COMPANY OF STATE GRID LIAONING ELECTRIC POWER SUPPLY +1
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