Separation and verification method of different electron-acceptor phosphorus-accumulating bacteria in activated sludge
A technology of electron acceptor and identification method, applied in the field of sewage biological treatment, to achieve the effect of strong practicability
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[0031] 1 According to the growth environment of microorganisms in the sewage biological treatment system, prepare 5 kinds of substrates, respectively No. 1 substrate (containing 13 C-sodium acetate 590mg / L), No. 2 base (containing 12 C-sodium acetate 590mg / L), No. 3 matrix (containing KH 2 PO 4 272mg / L), No. 4 matrix (containing KH 2 PO 4 272mg / L and NaNO 3 366mg / L) and No. 5 matrix (including KH 2 PO 4 272mg / L and NaNO 2 198mg / L).
[0032] 2 Take the sludge mixture in the biological treatment reactor, wash and centrifuge with distilled water three times, and dilute the centrifuged sludge to half the volume before the elutriation.
[0033] 3 Take 6 150mL conical flasks, labeled 1-6, respectively 13 C-sodium acetate is the carbon source and oxygen is the electron acceptor (No. 1); 13 C-sodium acetate is the carbon source, and nitrate is the electron acceptor (No. 2); 13 C-sodium acetate is the carbon source, and nitrite is the electron acceptor (No. 3); 12 C-sodium acetate is the carb...
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