Separation and verification method of different electron-acceptor phosphorus-accumulating bacteria in activated sludge

Abstract

The invention discloses a separation and verification method of different electron-acceptor phosphorus-accumulating bacteria in activated sludge, and belongs to the technical field of biological sewage treatment. The separation and verification method can separate the DNA of different electron acceptor phosphorus-accumulating bacteria in the activated sludge system of a sewage treatment plant, and analyze the characteristics of florae on the gene level by adopting a molecular biological method. The separation and verification method comprises the following steps: adopting 13C-sodium acetate as the carbon source of an experimental group and 12C-sodium acetate as the carbon source of a control group, conducting anaerobic phosphorus release for 2 hours, respectively using oxygen, nitrate and nitrite as electron acceptors, conducting aerobic or anoxic phosphorus uptake culture for 3 hours, conducting culture for 8 cycles totally, conducting ultrahigh-speed centrifugal separation and purification to obtain DNA samples with different density levels, and determining and analyzing the flora structures of the phosphorus-accumulating bacteria when the electron acceptors are different by a molecular biological method. The invention provides the method for analyzing the electron acceptors required by different phosphorus-accumulating bacteria, and provides a theoretical support for the denitrification-strengthened dephosphorization process of the sewage treatment plant practically.

Description

technical field [0001] The invention relates to a method for separating and identifying phosphorus-accumulating bacteria in activated sludge, which belongs to the technical field of sewage biological treatment and is used for separating and identifying different electron acceptor phosphorus-accumulating bacteria in sewage biological treatment systems. Background technique [0002] Biological nitrogen and phosphorus removal technology is currently the most widely used sewage treatment technology. The traditional phosphorus removal technology is based on the principles of anaerobic phosphorus release and aerobic phosphorus uptake. The phosphorus uptake process is under aerobic conditions. Phosphorus accumulating bacteria use molecular oxygen as an electron acceptor to consume PHB and exogenous substrates in the body for aerobic respiration. . Traditional denitrification and phosphorus removal technologies use carbon sources separately. The denitrification process uses carbon ...

AI Technical Summary

Problems solved by technology

[0007] Therefore, the present invention utilizes 13 C-sodium acetate was used as the carbon source of the experimental group, 12 C-sodium acetate was used as the carbon source of the control group, oxygen, nitrate and nitrite were respectively used as electron acceptors, anaerobic phosphorus release, aerobic or anoxic phosphorus absorption alternately, in situ culture of activated sludge, using ultra-high-speed density Gradient centrifugation was used to separate and purify the DNA, and then molecular biology techniques were used to measure the flora of phosphorus accumulating bacteria cultured by different electron acceptors, and the influence of different electron acceptors on the flora structure of phosphorus accumulating bacteria in the activated sludge system was analyzed. See related research reports