13 results about "Cloning genes" patented technology

The invention relates to a brassica napus drought-resistant cultivation method based on a target gene, which comprises the following steps: S1, cloning a BnaWRKY038 gene, and the nucleotide sequence of the BnaWRKY038 gene is as shown in SEQ ID NO: 1; s2, constructing an overexpression vector of the BnaWRKY038 gene, and transforming agrobacterium tumefaciens; s3, performing mediation transformation on the hypocotyl of the brassica napus by utilizing the agrobacterium to obtain a transgenic plant; s4, screening and identifying the transgenic plants to obtain the drought-resistant brassica napus with the overexpressed BnaWRKY038 gene. By obtaining the BnaWRKY038 overexpressed transgenic plants and based on phenotype results of the overexpressed transgenic rape plants, the drought resistance of the plants can be positively regulated under drought treatment of the rapes, a new choice is provided for screening dominant stress-resistant genes, and the drought-resistant brassica napus with the overexpressed BnaWRKY038 gene is obtained. The method has important application value in the field of plant genetic engineering, can effectively reduce economic loss caused by drought, and promotes cultivation of new varieties of drought-resistant rape.

This invention discloses a photoperiod regulation gene of foxtail grass. SvEhd1 Its application in shortening the research cycle of rice resistance to rice blast falls under the fields of genetic engineering and plant breeding. SvEhd1 The gene nucleotide sequence is shown in SEQ ID NO.1. This invention utilizes cloning... SvEhd1 Genes and their promoters were used to construct recombinant expression vectors, which were then transformed into the blast-susceptible rice variety "Taipei 309," resulting in transgenic rice materials with significantly shortened growth periods. Under natural and artificial long-day conditions, the heading time of the transgenic plants was significantly earlier than that of the wild type, the generation rate was accelerated, and the blast susceptibility of the original variety was not altered. The transgenic materials and methods provided by this invention can effectively shorten the experimental cycle for verifying the function of rice blast resistance genes and evaluating resistance, providing an efficient experimental system for rice disease resistance research.

This invention relates to the cloning and application of the salt tolerance gene LpWRKY75 in Lilium pumilum DC. The steps include: (1) cloning of the target gene; (2) construction of the expression vector; and (3) detection of physiological indicators in transgenic plants. This experiment used Lilium pumilum as the research object, cloning the LpWRKY75 gene from the cDNA of Lilium pumilum roots using homologous recombination technology; transforming tobacco by constructing an overexpression vector; and identifying the function of LpWRKY75 overexpression in improving tobacco salt stress through transgenic tobacco. The function of the LpWRKY75 gene in plant salt stress tolerance was preliminarily clarified, laying the foundation for further application of Lilium pumilum in lily breeding and providing important candidate gene resources for plant stress resistance breeding.

The application discloses a plant-derived plastic-degrading enzyme and a method for degrading PP plastic in vitro, and belongs to the technical field of enzyme engineering. The plant-derived plastic-degrading enzyme is a plant-derived HIS1 protease. The method for degrading PP plastic in vitro comprises the following steps: cloning a HIS1 gene from a plant by using an RT-PCR technology; connecting the gene to a prokaryotic expression plasmid by using a blunt-end cloning technology; introducing the gene into an expression platform of Bl21 (DE3) E. coli to express and purify the HIS1 protein; and degrading PP plastic in an enzyme reaction environment constructed in vitro. The HIS1 protein provided by the application can effectively degrade PP plastic, provides a new idea for degrading plastic by using plant enzymes, and has a good application prospect.

The application discloses a genetic element for enhancing lactic acid metabolic flux of actinomycetes, and belongs to the field of synthetic biology and microbial metabolic engineering. sucC The application successfully constructs a single expression element and a co-expression element by cloning genes from Streptomyces coelicolor sucD , and verifies that the SucC protein and the SucD protein both have lactic acid coenzyme A synthetase activity through in-vitro protein purification and enzyme activity determination, and the enzyme activity of a protein complex (SucC-SucD, SucCD) expressed by the co-expression element is significantly higher than that of the single protein, indicating that the two proteins have a synergistic effect; the genetic element can efficiently catalyze the combination reaction of lactic acid and coenzyme A in vitro, accelerates the lactic acid metabolic speed, provides a core genetic element for the modification of the lactic acid metabolic pathway of actinomycetes, enriches a synthetic biology tool library, and has important industrial application prospects and academic value.

This invention belongs to the field of genetic engineering technology, specifically relating to a BAMT gene that regulates salt-alkali tolerance in sunflowers and its application. The BAMT gene has the nucleotide sequence shown in SEQ ID NO.1. The amino acid sequence of the expressed protein of the BAMT gene is shown in SEQ ID NO.2. The specific primer pair nucleic acid sequences required for cloning the BAMT gene are shown in SEQ ID NO.3 and SEQ ID NO.4, or in SEQ ID NO.5 and SEQ ID NO.6. This invention also discloses the application of the BAMT gene regulating salt-alkali tolerance in sunflowers in a kit. To verify the difference in salt-alkali tolerance between two sunflower hybrids, hydroponic seedlings were treated with a saline-alkali solution. The results showed that the BAMT gene was continuously upregulated in K-27 and continuously downregulated in K-7, exhibiting a significant variety-specific expression pattern. This reveals the physiological and transcriptional regulatory characteristics of sunflowers in response to combined salt and alkali stress, and the identified BAMT gene can provide a reference for molecular breeding for salt-alkali tolerance.

The present application relates to the field of plant growth and development molecular biology, and particularly relates to genetic engineering application of gene OsDLN194, the gene OsDLN194 has the nucleotide sequence shown in SEQ ID NO.1, the protein sequence of the gene is SEQ ID NO.2, and the gene is located on the 7th chromosome of rice.The application of gene OsDLN194 in regulating at least one trait of rice tillering, yield per plant and nitrogen absorption.The present application obtains overexpression plants by cloning gene OsDLN194, constructing a transgenic vector, determining the phenotype of the transgenic plants, finds that the tiller number of the overexpression plants is significantly changed, and compared with wild type rice, the overexpression plants show the traits of increased tiller number, increased yield per plant, improved nitrogen absorption capacity and the like, which indicates that OsDLN194 gene plays an important regulatory function in the process of rice tillering, yield per plant and nitrogen absorption.

The present application relates to the field of biotechnology, in particular to a white flower carum gene MaUGT79 and its application, the present application is based on white flower carum genome data and BSA resequencing data, cloning MaUGT79 gene, using real-time quantitative PCR technology to analyze the expression pattern under drought stress, and construct the recombinant prokaryotic expression vector to verify MaUGT79 gene function, it is found that transgenic escherichia coli can catalyze scopolin to produce scopolamine; construct plant overexpression vector and RNAi vector to transform the gene into white flower carum hairy root, verify MaUGT79 gene to participate in scopolin synthesis, has the function of resisting drought stress, the present application lays the foundation for revealing the drought tolerance mechanism of MaUGT79 gene, provides gene resources and theoretical basis for plant drought tolerance molecular breeding.

The application is a divisional application of 202011444476.6. The application relates to the field of plant growth and development molecular biology, in particular to the application of gene OsPIN10b in plant root elongation, the gene OsPIN10b has a nucleotide sequence shown in SEQ ID NO:1, and the application of the gene OsPIN10b in regulating the length of plant roots. By cloning the gene OsPIN10b, a transgenic vector is constructed, overexpression and knockout transgenic materials are obtained, the phenotype of the transgenic materials is measured, the root morphogenesis of different materials is significantly changed, the overexpression materials have the characteristics of increased root length and the like compared with the knockout transgenic materials and wild type materials, and it is indicated that the OsPIN10b gene plays an important regulating function in the development process of rice root elongation.

The application discloses application and a method of a radish RsBBX9 gene in regulation of anthocyanin synthesis, and belongs to the technical field of plant genetic engineering. The application verifies the regulation of RsBBX9 on anthocyanin content by cloning the RsBBX9 gene, constructing an overexpression vector pFGC1008-RsBBX9 and a VIGS silencing vector pTY-S-RsBBX9, combining transient expression and gene silencing technology. The application further verifies the interaction of RsBBX9 and RsMYB1 by a yeast double-hybrid experiment, a BiFC experiment and a pull-down experiment, and the interaction cooperatively activates an anthocyanin synthesis path. The application provides an efficient technical scheme for radish variety improvement and anthocyanin production.

The invention discloses a cultivation method of drought-resistant brassica napus, and belongs to the technical field of biology. The method comprises the following steps: by taking brassica napus as a research object, extracting leaf RNA (Ribonucleic Acid), performing reverse transcription to obtain cDNA (Complementary Deoxyribonucleic Acid), cloning a BnaNCED3 gene, constructing an overexpression and silence vector, performing transformation verification by escherichia coli, and introducing agrobacterium; infecting the oilseed rape hypocotyl explant through agrobacterium tumefaciens, and carrying out co-culture, differentiation screening and rooting culture to obtain a transgenic plant; and finally, screening strains with high expression of BnaNCED3, and identifying the drought tolerance. By improving the expression level of the BnaNCED3 gene, the drought survival rate of the oilseed rape is remarkably improved, the water loss rate of leaves is reduced, and an efficient technical means is provided for cultivation of drought-resistant oilseed rape varieties.

This invention discloses a barley stripe disease resistance gene. Rdg2b This invention relates to the field of bioengineering technology and its applications. It describes the cloning of [specific species / organisms] from Ganbei No. 2 barley. Rdg2b The complete CDS segment of the gene was extracted, and an overexpression vector was constructed. Agrobacterium-mediated transformation was then performed to obtain overexpression-positive lines. Infection experiments with *Strombus striatum* showed that, compared to the wild type, [the expression was positive]. Rdg2b Overexpression lines showed significantly enhanced disease resistance. Real-time quantitative PCR analysis revealed that overexpression lines exhibited... Rdg2b Gene expression was significantly upregulated, confirming Rdg2b This invention positively regulates barley disease resistance. It provides important genetic resources for molecular breeding of barley for disease resistance.

The application discloses a fruit color regulation gene MdMYBS1 and application thereof, relates to the technical field of genetic engineering, and has the technical scheme as follows: a fruit color regulation gene MdMYBS1, the nucleotide sequence of the MdMYBS1 gene is shown in SEQ ID NO.1, and the amino acid sequence is shown in SEQ ID NO.2; after overexpression of the MdMYBS1 gene in apple callus, fruit, tissue culture seedlings and tomatoes, yellow color is deepened, the content of carotenoids is significantly increased, and the fruit color can be regulated; by cloning the gene MdMYBS1, the overexpression vector and the interference vector of MdMYBS1 are constructed, genetic transformation is carried out on apple fruit, callus or tissue culture seedlings as materials, the regulation mode of the gene in fruit carotenoid accumulation is revealed, and directional selection breeding of fruit yellow color regulation is facilitated.