Rice OsNAC coding sequence and application thereof

A coding and sequence technology used in the fields of molecular biology and genetic engineering

Inactive Publication Date: 2011-07-13
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Before the present invention was published, there was no disclosure or report of th

Method used

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  • Rice OsNAC coding sequence and application thereof
  • Rice OsNAC coding sequence and application thereof
  • Rice OsNAC coding sequence and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 Cloning of rice OsNAC gene.

[0031] The leaves of rice (Nipponbare) grown in the field were immediately frozen in liquid nitrogen for RNA extraction. according to OsNAC Primers SEQ ID NO.3 and SEQ ID NO.4 were designed for the ORF sequence. A band of about 1kb was amplified from rice cDNA by RT-PCR. The PCR product was recovered, connected to the vector pCR-Blunt, and sequenced.

Embodiment 2

[0032] Example 2 Sequence information and homology analysis of rice OsNAC gene.

[0033] Rice in the present invention OsNAC The length of the ORF of the gene is 1032bp, and the detailed sequence is shown in SEQ ID NO.1 (red bold characters mark the start codon and stop codon). The polypeptide encoded by the gene consists of 343 amino acid residues, the molecular weight is 36837.81 Daltons, and the isoelectric point is 7.47. See SEQ ID NO.2 for the detailed sequence.

[0034] in the rice OsNAC gene is based on ANAC092 The amino acid sequence of was obtained by Blast in the Non-redundant GeneBank CDS translations+PDB+SwissPort+Superdate+PIR database, and the homology between the two reached 73%.

[0035] ANAC092 It is involved in the senescence and lateral root formation of Arabidopsis, but there is no report on pollen development. ClustalX and MEGA2 have analyzed the amino acid sequences and ANAC092 and OsNAC Sequence homology comparison and evolutionary tree constr...

Embodiment 3

[0036] Example 3 Expression analysis of rice OsNAC under different organs, different hormones and different stress treatments.

[0037]Extract RNA from different organs (roots, stems, leaves, inflorescences) of "Nihonbare" during the flowering stage, in which young leaves that have not yet unfolded, mature leaves that have unfolded, and yellowed and unshrinked leaves are extracted according to the different growth stages and colors of the leaves. old leaves. Carry out Realtime PCR analysis, the results are as follows figure 2 ,show OsNAC Constitutively expressed in rice, the expression level is high in young leaves and senescent yellow leaves, implying that it is involved in the process of differentiation and senescence, and its traits involved in the process of plant senescence and its homologous genes in Arabidopsis ANAC092 resemblance. in addition, OsNAC The expression of is significantly induced by ABA, indicating that it may be involved in the stress response of ...

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Abstract

The invention discloses a coding sequence of an NAC family transcription factor expressed in rice and application thereof in pollen growth, belonging to the technical fields of molecular biology and genetic engineering. In the invention, the following processes of cloning of the NAC family transcription factor, analysis of a gene expression pattern, establishment of a transgenic carrier, phytotransformation, obtainment of a transgenic plant, and identification of a corresponding phenotype and a cellular level are comprised. The gene presented in the invention is expressed in Arabidopsis, and the obtained transgenic plant is abnormal in pollen growth and has a decreased ripening rate.

Description

technical field [0001] The invention belongs to the technical fields of molecular biology and genetic engineering, and specifically relates to the cloning of NAC family transcription factors expressed in rice, analysis of gene expression patterns, construction of transgenic vectors, plant transformation, acquisition of transgenic plants, and corresponding expression Type and cell level identification. Background technique [0002] Multiple stages of plant reproductive development affect the size and number of fruits and seeds. Thus, genes that control reproductive development can determine crop yields. The stages of plant reproductive development include the development of floral organs, stamen cell division and differentiation, meiosis, and pollen development. The genes regulating these stages may have a great impact on yield. The cloning and analysis of the functional genes of plant reproduction and development will provide candidate genes for improving crop yield. [0...

Claims

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Application Information

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IPC IPC(8): C07K14/415C12N15/29C12N15/82C12Q1/68A01H5/00
Inventor 明凤陈旭张文政唐雪蓉但晨
Owner FUDAN UNIV
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