Process for clone gene of virus host factor
A virus and gene technology, applied in the field of cloning virus host factor genes, can solve the problem of judging host or non-host
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment
[0027] The transgenic strain CN2 was inoculated on the PDA medium at 76.2umol m -2 the s -1 Light intensity, 12-hour light time per day, and 22-24°C conditions, cultured for 14 days, the sporulation amount is about 5×10 5 conidia / dish. Spread a certain number of conidia evenly on the surface of the PDA medium, irradiate it with ultraviolet light for a certain period of time with ordinary disinfection, place it at 22-24 ° C for 7-10 days, and observe the bacterium colonies formed by the surviving spores ( figure 1 ), pick non-white colonies for separate culture. Mutants with an orange-yellow phenotype ( figure 2 ). Validated by viral double-stranded RNA, some mutants lost viral double-stranded RNA ( image 3) , indicating a host factor gene mutant. A strain numbered 57 (named M57) was selected from these mutants, and a hyphal fusion experiment was performed with the virus-free EP155 strain, which proved that the mutant still had pre-virus mRNA activity. M57 was prepare...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com