Process for clone gene of virus host factor

A virus and gene technology, applied in the field of cloning virus host factor genes, can solve the problem of judging host or non-host

Inactive Publication Date: 2005-06-08
GUANGXI UNIV
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  • Process for clone gene of virus host factor
  • Process for clone gene of virus host factor
  • Process for clone gene of virus host factor

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[0027] The transgenic strain CN2 was inoculated on the PDA medium at 76.2umol m -2 the s -1 Light intensity, 12-hour light time per day, and 22-24°C conditions, cultured for 14 days, the sporulation amount is about 5×10 5 conidia / dish. Spread a certain number of conidia evenly on the surface of the PDA medium, irradiate it with ultraviolet light for a certain period of time with ordinary disinfection, place it at 22-24 ° C for 7-10 days, and observe the bacterium colonies formed by the surviving spores ( figure 1 ), pick non-white colonies for separate culture. Mutants with an orange-yellow phenotype ( figure 2 ). Validated by viral double-stranded RNA, some mutants lost viral double-stranded RNA ( image 3) , indicating a host factor gene mutant. A strain numbered 57 (named M57) was selected from these mutants, and a hyphal fusion experiment was performed with the virus-free EP155 strain, which proved that the mutant still had pre-virus mRNA activity. M57 was prepare...

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Abstract

A method for cloning the virus host factor gene by use of dual-chain RNA low-poison virus (chesnut blight bacterium system) includes such steps as light irradiating to induce the generation of conidia of chestnut phytophthora disease bacteria, inducing to obtain mutant, purifying, discriminating the virus carried by said mutant, testing the activity of its mRNA precursor, transforming and complementing of said mutant, discriminating, sequentially the exogenous DN'A fragment of the transformed plasmid with complem entary power, and configuring gene map.

Description

technical field [0001] The present invention relates to genetic engineering of viruses. Specifically, it relates to a cloning method for cloning virus host factor genes. Background technique [0002] Many important diseases of humans, animals and plants are caused by viruses. For the prevention and treatment of viral diseases in humans and animals, in addition to controlling the source of infection, it mainly relies on the immune response produced by the host's immune system to neutralize the virus. Vaccination is still the most effective way to deal with dangerous viral diseases. However, for viruses with variable immunogenicity, such as influenza virus, vaccination is often not very effective in preventing the outbreak and prevalence of such viral diseases. For some viral diseases without practical vaccines, such as AIDS caused by HIV, it is even more difficult to prevent and treat them. Most of the drugs currently used to treat viral diseases only relieve symptoms. A...

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Application Information

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IPC IPC(8): C12N1/21C12N15/09C12N15/63C12P19/34C12Q1/18C12Q1/68
Inventor 陈保善蓝秀万冯友军臧宁林海燕姜明国康宁
Owner GUANGXI UNIV
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