Artificial induced spawning of Phascolosoma esculenta

A delicacy and artificial induction technology, applied in animal husbandry and other directions, can solve the problems of low artificial reproduction rate, low sperm and egg volume, etc., and achieve the effects of high insemination rate, good effect, and high induction rate.

Inactive Publication Date: 2012-12-12
NINGBO UNIV
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AI Technical Summary

Problems solved by technology

However, this artificial reproduction test is only a preliminary exploration of small water bodies in the labora

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] The method of artificial oxytocin of D. coli, in August of the breeding season, select broodstock with complete body, strong vitality, flexible snout, light brown body surface, no disability, individual weight 2-4 g, among which the female broodstock The ratio of the number of male broodstock to the male broodstock is 1:1, the size of more than 80% of the oval oocytes in the body cavity of the female broodstock is not less than 110 -120 mm×140 -150 mm, and the size of 80% of the sperm in the body cavity of the male broodstock is not less than 20- 40mm × 20-50mm; 15 kg body was placed in a bamboo curtain, and the room was dried in the shade for 24 hours. The bamboo curtain was 2 m long, 1 m wide, and the edge was bent vertically upwards by 5 cm. The gap between the bamboo strips was not greater than 0.1 mm; After drying in the shade, sterilize by soaking in potassium permanganate solution with a concentration of 15-20 mg / L for 10-15 minutes, then rinse with filtered seawa...

Embodiment 2

[0013] It is basically the same as in Example 1, except that the broodstock is 14 kg, the stimulation time of drying in the shade is 21 hours, and the stimulation time of running water is 66 hours, and 86.5 million fertilized eggs are obtained, and the fertilization rate is about 95%.

Embodiment 3

[0015] It is basically the same as in Example 1, except that the parent body is 10 kg, the stimulation time of drying in the shade is 30 hours, and the stimulation time of running water is 58 hours, and 55 million fertilized eggs are obtained, and the fertilization rate is about 92%.

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Abstract

The invention discloses an artificial induced spawning of Phascolosoma esculenta, which comprises the following steps of: selecting female and male parents with higher egg cell and sperm maturity, firstly conducting shady stimulation for 20-30h in a bamboo curtain, then placing the female and male parents in a net basket and conducting flowing-water stimulation for 58-66h in an induced spawning pond with surface flowing speed being 0.2-0.3m/s, wherein the distance between the bottom of the net basket and the surface of water in the induced spawning pond is 5-8cm. Since the bamboo curtain is used for shady stimulation, the parents are evenly dried in the shade, the effect is good and the induced spawning is facilitated; since the net basket is used for centralized flowing-water stimulation, the induced spawning rate and the insemination rate are higher; and ethylene diamine tetraacetic acid can chelate with heavy-metal ions in seawater and the heavy-metal ions are prevented from adversely influencing insemination and embryo development. Therefore, due to shady stimulation in the bamboo curtain and the flowing-water stimulation in the net basket, sperms and egg cells produced by the parents are more, the insemination rate reaches more than 90 percent and the average quantity of fertilized eggs obtained per kilogram of parent is 5895,500.

Description

technical field [0001] The invention relates to the technology of oxytocin for animals, in particular to the artificial oxytocin method of D. palasis. Background technique [0002] Derma spp. (Latin Phascolosoma esculenta ) belongs to the genus Derma, commonly known as Haiding, which is distributed in the southeast coast and lives in caves in the intertidal zone. Cocoa stellate has high nutritional, economic and medicinal value, and is known as the "Cordyceps sinensis" in the sea. At present, the coastal areas of Zhejiang, Fujian, and Guangdong provinces are developing the characteristic aquaculture industry of D. coli, which is dioecious, and the sperm and eggs are discharged into the water for fertilization. The seedlings for breeding mainly rely on wild seedlings. Although Wu Hongxi et al. disclosed the "Artificial Propagation Test of Dermatophora coli" in "Marine Science" (March, 2010, 22-25), the artificial propagation test was to naturally air-dry the broodstock in ...

Claims

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Application Information

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IPC IPC(8): A01K67/033
Inventor 竺俊全丁理发金春华陈飞许式见徐利民
Owner NINGBO UNIV
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