Artificial induced spawning of Phascolosoma esculenta

Abstract

The invention discloses an artificial induced spawning of Phascolosoma esculenta, which comprises the following steps of: selecting female and male parents with higher egg cell and sperm maturity, firstly conducting shady stimulation for 20-30h in a bamboo curtain, then placing the female and male parents in a net basket and conducting flowing-water stimulation for 58-66h in an induced spawning pond with surface flowing speed being 0.2-0.3m / s, wherein the distance between the bottom of the net basket and the surface of water in the induced spawning pond is 5-8cm. Since the bamboo curtain is used for shady stimulation, the parents are evenly dried in the shade, the effect is good and the induced spawning is facilitated; since the net basket is used for centralized flowing-water stimulation, the induced spawning rate and the insemination rate are higher; and ethylene diamine tetraacetic acid can chelate with heavy-metal ions in seawater and the heavy-metal ions are prevented from adversely influencing insemination and embryo development. Therefore, due to shady stimulation in the bamboo curtain and the flowing-water stimulation in the net basket, sperms and egg cells produced by the parents are more, the insemination rate reaches more than 90 percent and the average quantity of fertilized eggs obtained per kilogram of parent is 5895,500.

Description

technical field [0001] The invention relates to the technology of oxytocin for animals, in particular to the artificial oxytocin method of D. palasis. Background technique [0002] Derma spp. (Latin Phascolosoma esculenta ) belongs to the genus Derma, commonly known as Haiding, which is distributed in the southeast coast and lives in caves in the intertidal zone. Cocoa stellate has high nutritional, economic and medicinal value, and is known as the "Cordyceps sinensis" in the sea. At present, the coastal areas of Zhejiang, Fujian, and Guangdong provinces are developing the characteristic aquaculture industry of D. coli, which is dioecious, and the sperm and eggs are discharged into the water for fertilization. The seedlings for breeding mainly rely on wild seedlings. Although Wu Hongxi et al. disclosed the "Artificial Propagation Test of Dermatophora coli" in "Marine Science" (March, 2010, 22-25), the artificial propagation test was to naturally air-dry the broodstock in ...

AI Technical Summary

Problems solved by technology

However, this artificial reproduction test is only a preliminary exploration of small water bodies in the laboratory. The amount of fertilized eggs obtained per kg of brood is low, and the artificial reproduction rate is low.