The invention relates to the field of biological culture equipment, in particular to an ecological agriculture high-density biological culture device. An escape-proof assembly is internally provided with a feeding assembly, and humidifying assemblies are arranged on the upper portions of the escape-proof assembly and the feeding assembly; a base window is formed in the middle of a base, runs through the base and is shaped like a rectangle; the feeding assembly comprises a sliding plate, sliding grooves, a rake assembly and a collecting assembly, the sliding grooves are fixedly formed in the upper portion of the base and fixed in the two sides of the edge of the base window, the sliding grooves are each shaped like C, the sliding plate is a rectangular metal plate, the sliding plate is matched with the base window, the two sides of the sliding plate are arranged in the sliding grooves, the sliding plate can slide in the sliding grooves, the collecting assembly is attracted to the rear portion of the sliding plate, and the rake assembly is arranged on the front portion of the sliding plate; each humidifying assembly comprises a main water delivery pipe, a branch pipe and an atomization nozzle. Accordingly, the structure is simple and reasonable, snails are not likely to climb out of a breeding room to be lost, and in the snail culture process, a cushion can be replaced conveniently in time.