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In-vitro fertilization liquid and preparation method thereof

A technology of fertilized fluid and culture fluid, applied in the field of in vitro fertilized fluid and its preparation, in vitro fertilized egg pre-culture fluid, which can solve the problems of unsuitable sperm and egg cell survival, affecting the development and growth of fertilized eggs, etc., to achieve strict detection and maintain the original Vibrant, simple and reasonable effect

Inactive Publication Date: 2014-11-26
SHENYANG JIERUI BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The present invention mainly solves the technical problems that the existing fertilized fluid is not suitable for the survival of sperm and egg cells, or affects the development and growth of fertilized eggs, and further provides a fertilized fluid that is completely suitable for the survival or growth of sperm, egg cells or fertilized eggs; the fertilized fluid can It ensures the capacitation of sperm and maintains its vitality, and enables the normal development and growth of fertilized eggs. It is safe to use and easy to visually judge whether it has deteriorated, providing a strong guarantee for assisted reproductive technology and avoiding risks.

Method used

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  • In-vitro fertilization liquid and preparation method thereof

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Embodiment 1

[0028] The in vitro fertilization solution of the present invention is a culture solution formulated by dissolving various solutes in ultra-pure water. The physical standards of the ultra-pure water are: ① the flow rate is 0.05Lpm-2.0Lpm, ② the resistivity at 25°C is 18.2MΩ.cm, and ③ the conductivity at 25°C is 0.055цS / cm; the chemical standard is: pyrogen Mass<0.001Eu / ml, particles≤1 / ml, TOC<5ppb. The ultra-pure water can also be replaced by water for injection. The plurality of solutes includes base solution solutes, bactericides, and stock solution solutes. Create an environment suitable for the survival of sperm, egg cells, and fertilized eggs through the deployment of basal fluid solutes and provide the nutrients needed for the growth of fertilized eggs. The base liquid solute includes sodium chloride, potassium chloride, magnesium sulfate, calcium chloride, sodium bicarbonate, sodium pyruvate, sodium lactate, taurine and sodium citrate. The stock solution solute is hu...

Embodiment 2

[0085] The in vitro fertilization liquid of the present invention is a culture liquid formulated by dissolving various solutes in ultra-pure water. The solutes include base solution solutes, bactericides and stock solution solutes. The base liquid solutes and stock solution solutes used in this embodiment are the same as in Example 1. For the content of each solute, refer to the "Formulation 2" column of Table 1. The specific content of the base liquid solutes in the culture solution is as follows: sodium chloride 101.5mmol / L, potassium chloride 4.69mmol / L, magnesium sulfate 0.20mmol / L, calcium chloride 2.04mmol / L, sodium bicarbonate 25.0mmol / L, sodium pyruvate 0.33mmol / L, sodium lactate 12.82mmol / L, Taurine 0.05mmol / L, sodium citrate 0.0005mmol / L; the content of the human serum albumin in the culture medium is 10g / L.

[0086] The bactericide in this culture solution is gentamicin, and its content in solution is 10mg / L.

[0087] No indicator was added to the culture solution...

Embodiment 3

[0091] The in vitro fertilization liquid of the present invention is a culture liquid formulated by dissolving various solutes in ultra-pure water. The solutes include base solution solutes, bactericides and stock solution solutes. The base liquid solutes and stock solution solutes used in this embodiment are the same as in Example 1. For the content of each solute, refer to the column of "Formulation 3" in Table 1. The specific content of the base liquid solutes in the culture solution is as follows: sodium chloride 106.6mmol / L, potassium chloride 4.92mmol / L, magnesium sulfate 0.21mmol / L, calcium chloride 2.14mmol / L, sodium bicarbonate 26.25mmol / L, sodium pyruvate 0.35mmol / L, sodium lactate 13.47mmol / L, Taurine 0.053mmol / L, sodium citrate 0.000525mmol / L; the content of the human serum albumin in the culture medium is 10.5g / L.

[0092] The bactericide in this culture solution is gentamicin, and its content in solution is 10.5mg / L.

[0093] The indicator added in the culture ...

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Abstract

The invention discloses in-vitro fertilization liquid and a preparation method of the in-vitro fertilization liquid. By adopting the in-vitro fertilization liquid and the preparation method of the in-vitro fertilization liquid, disclosed by the invention, the technical problem that existing fertilization liquid is not suitable for the survival of sperm and egg cells or can influence the development and growth of fertilized eggs can be solved. The in-vitro fertilization liquid is a culture solution obtained by dissolving a variety of solutes into ultra-pure water by virtue of blending, wherein a basic solution solute comprises sodium chloride, potassium chloride, magnesium sulfate, taurine, sodium citrate and the like; a sterilizing agent is gentamicin; a stock solution solute is human serum albumin; the blended culture solution is filtered by adopting a filter membrane with a thickness of 0.2 microns again for performing sterilization treatment under a super clean bench. The in-vitro fertilization liquid disclosed by the invention contains a variety of organic or inorganic components which are suitable for the survival of the sperm and egg cells and are suitable for the growth and development of the fertilized eggs, thereby providing a guarantee for the cultivation of test-tube babies.

Description

technical field [0001] The invention belongs to the technical field of artificial assisted reproduction, and relates to an in vitro fertilized egg pre-culture liquid, which is an in vitro fertilized liquid and a preparation method thereof. Background technique [0002] The current increase in human infertility has prompted the development of test-tube baby technology. In the test-tube baby technique, it is necessary to collect good sperm and maternal egg cells for fertilization and pre-culture. This culture process is closely related to the fertilized fluid, and the quality of the fertilized fluid determines the success or failure of the test-tube baby. The purpose of in vitro fertilization is mainly to provide nutrients for the survival of sperm and eggs and for the development of fertilized eggs, as well as to create a reasonable environment for the survival, growth and development of sperm, eggs and fertilized eggs. The split culture of fertilized eggs by in vitro fertil...

Claims

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Application Information

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IPC IPC(8): C12N5/073C12N5/075C12N5/076
Inventor 王维华周淑梅黄永军
Owner SHENYANG JIERUI BIOTECH
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