The invention relates to a PRP preparation device and a PRP preparation method, the preparation device comprises a
pipe body, an upper cover, a lower cover and a sealing plug, the
pipe body comprises an upper section
pipe, a middle pipe and a lower section pipe, the upper cover and the upper section pipe are matched to form an upper cavity, the upper cover can move up and down to enable the upper cover to be switched between a first position and a second position, and the upper cover is provided with a
liquid injection and suction hole, a liquid suction pipe and a sealing piece; the lower cover is matched with the lower section pipe to form a lower cavity, and the lower cover can move up and down to adjust the volume of the lower cavity; the sealing plug is used for controlling opening and closing of the
liquid injection and suction hole and the liquid suction pipe; the part, located in the upper cavity, of the
pipette has a set length, and the set length enables the
pipette to be located in the
platelet-poor
plasma layer and keep a gap with the
platelet-rich
plasma layer when the
pipette is located at the second position, and enables the port of the pipette to be located outside the
platelet-rich
plasma when the port of the pipette is located at the first position. By applying the device, the PRP concentration can be directly adjusted in the preparation device, and the damage to platelets can be avoided when the PRP is extracted.