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Automatic egg cell capturing and culturing device

An automated device and technology for egg cells, which are applied in the fields of egg cell capture, culture and reproduction, can solve the problems of difficulty in capturing and replacing cell culture medium, increase operating costs, etc., achieve automation, wide application prospects and practical value, and are obviously novel. The effect of functionality and practicality

Active Publication Date: 2014-04-02
XIDIAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the small size and light weight of the cells, they are in a state of irregular movement in the culture medium, and it is difficult to capture them manually and replace the cell culture medium, which greatly increases the operating cost in the process of industrial batch culture

Method used

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  • Automatic egg cell capturing and culturing device
  • Automatic egg cell capturing and culturing device
  • Automatic egg cell capturing and culturing device

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Embodiment Construction

[0031] The specific embodiments of the present invention will be further described below in conjunction with the accompanying drawings.

[0032] like figure 1 As shown, the egg cell capture and culture automation device of the present invention is divided into four mechanisms, including a culture solution supply mechanism arranged on the support 12 through motor linkage, a cell capture mechanism is provided below the culture solution supply mechanism, and a waste liquid is disposed below the cell capture mechanism. The cleaning mechanism, the waste liquid cleaning mechanism and the culture solution supply mechanism are connected with the control mechanism through wires. It is used to capture the egg cells contained in the cell culture fluid; the egg cells on the capture mechanism are automatically supplied with cell culture fluid through the culture fluid supply mechanism; finally, the old cell culture fluid is removed from the cell capture mechanism through the waste liquid r...

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Abstract

The invention discloses an automatic egg cell capturing and culturing device. The automatic egg cell capturing and culturing device comprises a culture solution supply mechanism which is arranged on a bracket and is linked by an electric motor, wherein the culture solution supply mechanism is connected with the motor through the bracket; the electric motor is connected with a rotary wheel disc connected by a bearing; injectors which penetrate through the disc surface are annularly and uniformly distributed on the rotary wheel disc; a cell capturing mechanism is arranged below the culture solution supply mechanism and comprises a base formed by a rectangular frame; a double-layer hole film embedded on the base is arranged on an enclosure of the rectangular frame; a waste liquid removal mechanism is arranged below the cell capturing mechanism and comprises a flask connected to the lower side of a three-neck container; a control mechanism comprises a control board which is respectively connected with a micro pump, the electric motor and a motor through wires. According to the device, batch capture of egg cells and liquid transfer culture automation are realized, and a technical scheme which is low in cost and easy to operate is provided for realizing in-vitro culture of egg cells, fertilization and early culture of embryos.

Description

technical field [0001] The invention relates to the field of egg cell capture, cultivation and reproduction, in particular to an automatic device for egg cell capture and culture. Background technique [0002] With the advancement of science and technology, "test-tube animals", "test-tube babies", "abdominal pregnancy", in vitro fertilization, etc. are often reported, and the in vitro culture, fertilization, and reproduction of egg cells have gained more and more attention and investment. "Fertility is a normal human physiological phenomenon that allows us to pass it on from generation to generation, but some people are infertile because of lesions in the reproductive organs or defects in the germ cells. The number of sperm in men is too low and the sperm cannot swim forward, just indiscriminately Twisting, unable to combine with eggs. In animal breeding and reproduction, the fertility rate of some rare animals is low. It is very disadvantageous to increase the production o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00
CPCC12M21/06C12M23/40C12M25/04C12M29/04C12M33/14C12M41/00
Inventor 罗勇马娟王芳林徐亚兰
Owner XIDIAN UNIV
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