The invention discloses a glyphosate and glufosinate ammonium compounded herbicide composition. The glyphosate and glufosinate ammonium compounded herbicide composition is prepared from glyphosate, glufosinate ammonium, levo-alpha-terpilenol and a traditional Chinese medicine additive; the traditional Chinese medicine additive comprises callistemon rigidus, erodium stephanianum willd, geranium, artemisia annua, cape marigold, Chinese pulsatilla root, coptis chinensis, xanThium sibiricum, sophora alopecuroide, rheum officinale, garlic, folium artemisiae argyi, honeysuckle and houttuynia cordata; the composition can further be matched with an agriculturally pharmaceutically acceptable carrier to prepare preparations like a missible oil, a microemulsion, an emulsion oil in water, a suspendingagent, a wettable powder, a water dispersible granule, a suspoemulsion, a granule, an aqueous solution and a powder, and the composition is convenient to use and store. The glyphosate and glufosinateammonium compounded herbicide composition has an obvious synergistic interaction, is low in toxicity, high in efficiency, and long in lasting period, and is organic and environmentally friendly, theuse amount of a herbicide is reduced, the use cost and the environment pollution are reduced, and the grassy weeds, the sedge weeds and the broadleaf weeds in the forest land and the non-cultivated land are effectively prevented and killed.