Water extraction method of ricinine, application of extract and toxicity evaluation method of ricinine

An evaluation method and technology of ricin, applied in the directions of application, botanical equipment and method, biocide, etc., can solve problems such as low pupation rate, and achieve the effects of low human and animal toxicity, simple operation, and high fumigation killing rate.

Active Publication Date: 2014-12-03
TIANJIN UNIV OF SCI & TECH
View PDF1 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have found that the crude extract of ricin has different degrees of killing effects on canopy caterpillars, peach aphids, diamondback moths and other insects, among which it has a strong inhibitory effect on diamondback moth pupation, and the pupation rate is lower than 30%.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Water extraction method of ricinine, application of extract and toxicity evaluation method of ricinine
  • Water extraction method of ricinine, application of extract and toxicity evaluation method of ricinine
  • Water extraction method of ricinine, application of extract and toxicity evaluation method of ricinine

Examples

Experimental program
Comparison scheme
Effect test

specific example 1

[0067] Concrete example 1: do solvent with hot water, ricinine is mixed with 1mg / mL liquid medicine, the circular filter paper that diameter is 9cm is cut into identical two halves, respectively drips the ricinine solution 0.5mL of 0.5mL and An equal amount of distilled water was used as a control. After the solvent was evaporated to dryness, the two halves of the filter paper were glued with adhesive paper and placed in a petri dish with a diameter of 9 cm. Inject 30 corn weevil adults into each dish, move the petri dish into the bioassay room (temperature 30°C±1°C, humidity: 75%±5%), observe the distribution of corn weevil after 72 hours, and the repellent rate is 100% .

specific example 2

[0068] Concrete example 2: do solvent with hot water, ricinine is mixed with 1mg / mL liquid medicine, the circular filter paper that diameter is 9cm is cut into identical two halves, respectively drips 0.5mL of ricinine solution of 0.5mL, An equal amount of distilled water was used as a control. After the solvent was evaporated to dryness, the two halves of the filter paper were glued with adhesive paper and placed in a petri dish with a diameter of 9 cm. Inject 30 adults of Tribulus chinensis into each dish, move the petri dish into the bioassay room (temperature 30°C±1°C, humidity: 75%±5%), and investigate the distribution of Triniprobium redifolia after 72 hours, and its repellent rate was 98.7%.

[0069] 2. Determination of the effect of ricinine on the fumigation of stored grain pests, using the sealed fumigation method of jars, the steps are as follows:

[0070] In a 1000mL wide-mouth bottle, hang a 3cm x 11cm piece of filter paper in the center, insert 30 test insects,...

specific example

[0082] Specific example: after nematodes are synchronized to L4 stage, they are transferred to NGM plates containing ricinine biopesticides at corresponding concentrations of 2.0mg / mL, 1.5mg / mL, 1.0mg / mL, and 0.5mg / mL. The experimental plate contained 12.5 mg / L 5-Fu and was cultured at 20°C. The number of experimental samples in each group is about 60, 24, 48, 72, 96, 120h. Such as image 3 Shown, when the concentration of ricinine extract is 1.5mg / mL, when the time of action is 120h, the mortality of Caenorhabditis elegans is 100%, and the concentration of ricinine extract is 2.0mg / mL, and when the time of action is 96h, The mortality rate of C. elegans is 100%. The higher the concentration of ricinine extract, the earlier the death time of all C. elegans, indicating that the greater the concentration of ricinine extract, the stronger the toxicity.

[0083] 4. The test of the influence of ricinine on nematode reproductive ability, the method is as follows:

[0084] Pick L...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a water extraction method of ricinine, application of extract and a toxicity evaluation method of ricinine. Magnetic-force heating and mixing auxiliary ultrasonic extraction technology is adopted, water is used as extraction solvent, the method of extracting high-purity ricinine from castorseed shells and castor old leaves is utilized, ricinine is used for controlling grain storing pests such as sitophilus zeamais and tribolium castaneum, caenorhabditis elegans are used for toxicity evaluation of ricinine, and lethality, fecundity, body length and activity of enzyme inside body of caenorhabditis elegans are determined. The water extraction method of ricinine, application of extract and the toxicity evaluation method of ricinine are simple in operation and low in cost, reduce use of organic reagents, protect the environment, and provide necessary conditions for large-scale industrial production of ricinine biological pesticides due to the fact that extraction solution can be directly used for controlling grain storing pests.

Description

technical field [0001] The invention belongs to the technical field of ricinine extraction, in particular to a water extraction method of ricinine, an application of the extract and a toxicity evaluation method of ricinine. Background technique [0002] Corn weevil, Sitophilus zeamais (Motschusky), belongs to Coleoptera Weevilidae, is one of the main pests of stored grain in my country, and is also an important pest of stored grain worldwide. It parasitizes in a variety of foods, with corn, wheat, and sorghum being the most affected. It mainly harms the old grains stored for 2-3 years, the adults eat the grains, and the larvae eat the grains. At present, the main control method is to use malathion to trap and kill adults. It has been proved that plant extracts such as fennel, star anise, black pepper powder, tangerine oil, menthol, camphor, eucalyptus oil, and pyrethrum have different degrees of control effects on stored grain pests. [0003] Tribolium castaneum (Herbst),...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): C07D213/85A01N65/18A01P5/00G01N33/00
Inventor 王昌禄魏巍王玉荣陈勉华李风娟李贞景
Owner TIANJIN UNIV OF SCI & TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap