Compound composition of osthole and thiafuramide and application thereof
A compound composition and technology of thifluzamide, applied in the fields of application, biocide, animal repellent, etc., can solve the problems that have not been reported, and achieve the effects of reducing risk, reducing the amount of field use, and low toxicity to humans and animals
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Embodiment 1
[0019] Indoor toxicity test and compound ratio screening
[0020] 1.1 Drugs to be tested
[0021] 94.7% osthole original drug, provided by the Institute of Food Quality and Safety, Jiangsu Academy of Agricultural Sciences.
[0022] 96% Thiofuramide technical product, product of Zhaoyuan Sanlian Far East Chemical Co., Ltd., Shandong Province.
[0023] 1.2 Tested strains
[0024] The target of the test is the strain sample of wheat sheath blight (Rhizoctonia cerealis Vander Hoeven) which was isolated from diseased roots in the field, cultivated and purified the first-generation strain, and stored in a 4°C refrigerator for later use.
[0025] 1.3 Instruments and equipment
[0026] There are mainly electronic balances, ultra-clean workbenches, biological incubators, petri dishes, micropipettes, etc.
[0027] 1.4 Experimental method
[0028] The Mycelium growth rate test recommended by the FAO was used.
[0029] 1.4.1 Drug compounding
[0030] Table 1 Compound ratio of ostho...
Embodiment 2
[0061] field plot experiment
[0062] According to the indoor compound ratio screening experiment, the optimal ratio of osthole:thiafuramide=1:8 was selected, and we prepared a 9% osthole·thiafluxamide compound composition. According to the "Pesticide Field Efficacy Test Guidelines (2)" edited by the Ministry of Agriculture's Pesticide Control Institute, the field efficacy test of 9% osthole·thiafuramide compound composition on wheat sheath blight was designed .
[0063] 2.1 Drugs to be tested
[0064] 240 g / L Thiofuramide Suspending Concentrate 54g / ha, Japan Nissan Chemical Industry Co., Ltd.;
[0065] 1% osthole water emulsion 22g / ha, Chengdu Lvjin Biotechnology Co., Ltd.
[0066] 9% osthole Thiofuramide 36, 45, 54g / ha, prepared by Yantai Academy of Agricultural Sciences.
[0067] 2.2 Control drug
[0068] 250 g / L propiconazole EC 150g / ha, the product of Swiss Syngenta Crop Protection Co., Ltd.
[0069] 2.3 Community design
[0070] There are 7 treatments in total in ...
Embodiment 3
[0085] safety test
[0086] The content of osthole and thiafuramide in the compound composition is less than that of commercially available products, so its toxicity to humans and animals and the safety of crops meet the requirements. The results of the toxicity test of the accredited institution show that the compound fungicide belongs to the slightly toxic pesticide:
[0087] Acute oral toxicity in rats ♀>5000mg / kg;
[0088] Acute dermal toxicity in rats ♀>20000mg / kg.
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