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Strain of fusarium chlamydosporum and application thereof

A technology of Fusarium and strains, applied in the application, fungicides, fungi and other directions, can solve the problems of long residual period, resistance to plant pathogenic fungi and nematodes, application limitations, etc., to achieve good nematicidal activity, good application prospects, good inhibitory effect

Active Publication Date: 2017-05-24
青岛秾贝尔生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the prevention and control of plant diseases mainly includes chemical pesticide control, crop rotation and the use of resistant varieties, etc., all of which have certain limitations.
Especially for chemical pesticide control, it is easy to make plant pathogenic fungi and nematodes resistant to pesticides, and the residual period is long, so the application is limited

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1: Isolation and Identification of Fusarium Chlamydoides QJP1 Strain

[0019] (1) Strain isolation

[0020] The bacterial strain QJP1 of the present invention is isolated from soil by methods such as plate dilution coating and streaking. (1) Soil sample collection: the collection site is the Suaeda salsa wetland in Jiaozhou Bay, Qingdao, and the 5-point sampling method is adopted. (2) Separation step: Weigh 1g of saline-alkali soil sample into 100mL of sterile water, place in a shaker at 30°C and 150r / min for 10min, and dilute to 10 -2 、10 -3 、10 -4 Concentration; draw 100 μL of the above dilutions, and spread evenly on the LB double-antibody medium plate containing 3.5% sea salt, and apply three parallels for each gradient; after culturing at 30°C for 2 days, pick microorganisms of different forms on the surface of the medium colony, and streaked on the LB double-antibody medium plate containing 3.5% sea salt, and observed the growth of the colony regularly...

Embodiment 2

[0026] Embodiment 2: Fusarium chlamydobacterium strain QJP1 fermentation culture

[0027] (1) Fermentation culture

[0028] According to the conventional culture method of microorganisms, a small amount of Fusarium chlamydoides strain QJP1 preserved on the slant of PDA medium was picked, inoculated on the surface of PDA plate, and cultured at 28°C for 5 days, as the strain of large-scale fermentation culture, ready for use.

[0029] PDA medium: 200 g of potatoes, 20 g of glucose, 12 g of agar, 1000 mL of distilled water, and adjust the pH to 7.0.

[0030] (2) Cut the strains on the surface of the above-mentioned PDA plate (the amount is 1 / 4 of the plate), inoculate it into a sterilized Erlenmeyer flask filled with PDB medium, culture it on a shaker at 28°C and 120rpm for 10 days, and set aside .

[0031] PDB medium: 200g potatoes, 20g glucose, 1000mL distilled water, adjust the pH to 7.0.

[0032] (3) Preparation of fermentation products

[0033] The above-mentioned cultur...

Embodiment 3

[0034] Embodiment 3: antibacterial activity

[0035] The microdilution method was used to determine the antibacterial activity of the fermentation product of Fusarium chlamydoides strain QJP1 against Fusarium wilt and Citrus anthracnose.

[0036] 1) Preparation of bacterial suspension

[0037]Inoculate the fungus to be tested on the surface of PDA medium and culture at 28°C for 72 hours, draw 2 mL of sterile 0.85% NaCl solution (containing 0.25% Tween-20) to wash the culture, and gently scrape off the colony with a glass scraper. Draw an appropriate amount of bacterial suspension into a sterile test tube and adjust to 0.5 McFarland turbidity (equivalent to 1.5×108CFU / mL) for later use;

[0038] 2) Sample preparation

[0039] Take a certain amount of sample to be tested (the elution fraction II of the fermentation product in Example 2), dissolve it in 100 μL 50% DMSO, mix thoroughly, draw 50 μL of the sample solution into another centrifuge tube, and then add 50 μL of 50 %DM...

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Abstract

The invention discloses a strain of fusarium chlamydosporum and an application thereof. The strain is currently collected in the CGMCC (China General Microbiological Culture Collection Center) on December 9th, 2016, with a collection number of CGMCC No.13198. After fermentation and extraction, the strain has relatively high inhibiting activity on plant pathogenic fungi such as cotton wilt fusarium and colletotrichum glecosporioides, and the minimum inhibiting concentration (MIC) is 4mu g / mL and 8mu g / mL respectively; moreover, the strain also has relatively good nematicidal activity against American ginseng root-knot nematodes, and the fatality rates of 50-times and 100-times diluents against American ginseng root-knot nematodes are 100% and 83% respectively; and thus, the strain has good application prospects in plant disease control.

Description

technical field [0001] The invention belongs to the field of microbial pesticides. Specifically, the present invention relates to a strain of Fusarium chlamydoides and its application. Background technique [0002] Various plant diseases caused by phytopathogenic fungi seriously threaten my country's crop production, and the annual economic losses are incalculable. For example: Cotton Fusarium wilt occurs to varying degrees in the main cotton-producing areas of my country. The disease can occur at the seedling stage, and can even cause the entire cotton plant to wither and die, seriously affecting the yield and quality of cotton. [0003] Phytopathogenic nematodes are also one of the common plant diseases. In my country, they mainly harm economic crops such as tobacco, notoginseng, cotton, peanut, and American ginseng, and have become one of the important factors that endanger the output of economic crops. According to statistics, crop losses caused by plant pathogenic nem...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12P1/02A01N63/04A01P3/00A01P5/00C12R1/77
CPCA01N63/30C12N1/14C12P1/02C12N1/145C12R2001/77
Inventor 杜丰玉肖琳周远明牛赡光王学军
Owner 青岛秾贝尔生物工程有限公司
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