Cryoprotectant and application thereof, sperm refrigerating fluid and preparation method of sperm refrigerating fluid

Abstract

The invention relates to the technical field of assisted reproduction, and discloses a cryoprotectant and an application thereof, a sperm refrigerating fluid and a preparation method of the sperm refrigerating fluid. The sperm refrigerating fluid comprises a cryoprotectant, a composition and a basic culture solution, wherein the cryoprotectant comprises 0.3 to 1.1 mg/L of paclitaxel, 16 to 22 g/Lof trehalose, 7 to 13 g/L of cane sugar and 80 to 250 mL/L of glycerinum; the composition comprises 12 to 26 mg/L of resveratrol, 0.2 to 1.9 mg/L of reduced glutathione, 436 to 775 mg/L of lecithin, 0.5 to 1.6 mg/L of cholesterol, 9 to 17 mg/L of ATP sodium salt, 11 to 28 mg/L of heparin, 93 to 299 mg/L of phosphatidylserine and 5 to 25 mg/L of coenzyme Q10. The sperm refrigerating fluid can reduce damage of sperms in low-temperature freezing and freezing resuscitation processes, improve vitality of the sperms after freezing resuscitation, prolong survival time of the sperms after freezing resuscitation, promote sperm capacitation and enhance penetrating fertilization capacity of the sperms to egg cells. Thus, the fertilization success rate and blastocyst formation rate of the ova are improved, and finally the effect of improving the pregnancy success rate of the in-vitro fertilization assisted reproduction technology is achieved.

Description

technical field [0001] The invention relates to the technical field of assisted reproduction, in particular to a cryoprotectant and its application, a sperm freezing liquid and its preparation method. Background technique [0002] In vitro fertilization (in vitro fertilization, IVF), also known as test-tube baby, is a method of assisted reproductive therapy, specifically refers to the fertilization of sperm and eggs under specific environmental conditions outside the body after the eggs and sperm are taken out of the body respectively. Fertilized eggs are formed, and the fertilized eggs are continuously cultured and developed in vitro to embryos at the blastocyst stage, and then the embryos are transferred back to the mother's uterus for pregnancy and development into fetuses. [0003] In the assisted reproductive technology of in vitro fertilization, men can freeze their own sperm in medical institutions with corresponding qualifications based on the consideration of future...

AI Technical Summary

Problems solved by technology

[0004] At present, the existing sperm freezing solutions on the market are mainly divided into two categories, namely those containing egg yolk and those without egg yolk, but both of them have some disadvantages: on the one hand, since the egg yolk contains not only lecithin, but also many complex Components. Granular substances will appear after the sperm cryopreservation with yolk-containing sperm freezing liquid, which will affect the further processing process. At the same time, because the yolk is a heterologous substance, there is still a certain biological risk; on the other hand, the sperm without yolk The cryoprotective effect on sperm is limited because the cryogenic solution only contains basic salt ions, a small amount of amino acids, and a single sperm cryoprotectant, and there is a lot of room for improvement

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