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Operating system and method for egg cell micro-injection

A technology of microinjection and operating system, which is applied in the field of operating system for egg cell microinjection, can solve problems such as complexity and egg cell damage, and achieve the effects of strong operational flexibility, reduced trauma, and easy observation

Active Publication Date: 2018-08-17
BEIJING INSTITUTE OF TECHNOLOGYGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the above problems, the present invention provides an operating system and method for microinjection of egg cells. The vibration of the glass needle makes the water form a vortex, thereby driving the egg cells to move and / or rotate, and then injecting the egg cells, effectively solving the problem of contact. The complexity of micro-manipulation movement control, and the problem of damage to egg cells caused by direct contact

Method used

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  • Operating system and method for egg cell micro-injection
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  • Operating system and method for egg cell micro-injection

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Embodiment 1

[0039] see figure 1 , which is a schematic structural diagram of an operating system for egg cell microinjection provided in the embodiment of the present application.

[0040] An operating system for egg cell microinjection, including a micro-nano manipulation device and a cell adsorption device;

[0041] The micro-nano operation device includes a three-coordinate operation platform 1, a manual platform 2, a metal rod 3, a vibration mechanism 4, a metal connector 5 and a glass needle 6; the manual platform 2 is fixed on the three-coordinate operation platform 1, and the three The coordinate operation platform 1 is used to adjust the position of the manual platform 2; one end of the metal rod 3 is fixed on the manual platform 2, and the other end is vertically connected with the vibration mechanism 4; the glass needle 6 is separated from the vibration mechanism 4 through the metal connector 5. One end of the metal rod 3 is connected vertically, and the tip of the glass needle...

Embodiment 2

[0058] Based on the above-mentioned embodiments, the embodiment of the present application provides an operation method for egg cell microinjection. see Figure 5 , which is a flow chart of an operation method for egg cell microinjection provided in the embodiment of the present application.

[0059] An operation method for egg cell microinjection, applied to an operating system for egg cell microinjection, the operating system includes a micro-nano operating device and a cell adsorption device 10, comprising the following steps:

[0060] S501: putting the egg cell 8 into the water drop 7, and placing the water drop 7 containing the egg cell 8 in the field of view of the microscope through the glass slide 9;

[0061] S502: Adjust the manual platform 2, place the tip of the glass needle 6 into the water drop 7, wherein the tip does not touch the egg cell 8;

[0062] see Figure 6 , which is a schematic diagram of the relative positions of a glass needle and an egg cell provi...

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Abstract

The invention provides an operating system for egg cell micro-injection. High-speed rotation of a glass needle, which is kept in water, is driven via vibration of a vibrating mechanism, so that an eddy current is caused in the water, subsequently, egg cells are moved and rotated, and finally a purpose of conducting egg cell injection is achieved; in comparison with existing systems that egg cellsare operated via micro-operation manipulators, the operating system provided by the invention can avoid mechanical contact with the egg cells, so that direct or indirect injury to the egg cells is avoided; and the end of the glass needle can conduct continuous rotation on the egg cells in an in-situ mode, so that the egg cells can be easily controlled within the range of a view field and observation can be facilitated; and in comparison with existing systems that the egg systems are operated within micro-fluidic chips, the operating system provided by the invention, which is free from limitation of operating tasks and the size of the egg cells, is strong in operating flexibility.

Description

technical field [0001] The invention belongs to the technical field of micro-nano operation, and in particular relates to an operating system and method for egg cell microinjection. Background technique [0002] Cell microinjection is an important part of single cell analysis, and it is a basic way to transfer genes into cells. Cell injection is required for cloning and transgenic techniques. Among them, cell movement and cell rotation are the most important and basic operations in cell microinjection. Before and after moving cells to a certain position, they need to be rotated and oriented with multiple degrees of freedom to adjust their posture to meet the needs of the injection position. Especially for egg cells, the nuclei are located at the edge of the cell and distributed asymmetrically, so it needs to be repositioned to the observable position of the microscope focal plane during cell injection. Therefore, moving and rotating technology plays an important role in the...

Claims

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Application Information

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IPC IPC(8): C12M1/00C12N15/89
CPCC12M35/00C12N15/89
Inventor 刘晓明唐小庆李鹏云陈俊男董胜男黄强新井建生
Owner BEIJING INSTITUTE OF TECHNOLOGYGY
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