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A kind of operating system and method for egg cell microinjection

A technology of microinjection and operating system, applied in the field of operating system of egg cell microinjection, can solve the problems of complexity and egg cell damage, and achieve the effect of strong operation flexibility, reducing trauma and avoiding damage

Active Publication Date: 2021-07-27
BEIJING INSTITUTE OF TECHNOLOGYGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the above problems, the present invention provides an operating system and method for microinjection of egg cells. The vibration of the glass needle makes the water form a vortex, thereby driving the egg cells to move and / or rotate, and then injecting the egg cells, effectively solving the problem of contact. The complexity of micro-manipulation movement control, and the problem of damage to egg cells caused by direct contact

Method used

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  • A kind of operating system and method for egg cell microinjection
  • A kind of operating system and method for egg cell microinjection
  • A kind of operating system and method for egg cell microinjection

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Embodiment 1

[0039] see figure 1 , which is a schematic structural diagram of an operating system for egg cell microinjection provided in the embodiment of the present application.

[0040] An operating system for egg cell microinjection, including a micro-nano manipulation device and a cell adsorption device;

[0041] The micro-nano operation device includes a three-coordinate operation platform 1, a manual platform 2, a metal rod 3, a vibration mechanism 4, a metal connector 5 and a glass needle 6; the manual platform 2 is fixed on the three-coordinate operation platform 1, and the three The coordinate operation platform 1 is used to adjust the position of the manual platform 2; one end of the metal rod 3 is fixed on the manual platform 2, and the other end is vertically connected with the vibration mechanism 4; the glass needle 6 is separated from the vibration mechanism 4 through the metal connector 5. One end of the metal rod 3 is connected vertically, and the tip of the glass needle...

Embodiment 2

[0058] Based on the above-mentioned embodiments, the embodiment of the present application provides an operation method for egg cell microinjection. see Figure 5 , which is a flow chart of an operation method for egg cell microinjection provided in the embodiment of the present application.

[0059] An operation method for egg cell microinjection, applied to an operating system for egg cell microinjection, the operating system includes a micro-nano operating device and a cell adsorption device 10, comprising the following steps:

[0060] S501: putting the egg cell 8 into the water drop 7, and placing the water drop 7 containing the egg cell 8 in the field of view of the microscope through the glass slide 9;

[0061] S502: Adjust the manual platform 2, place the tip of the glass needle 6 into the water drop 7, wherein the tip does not touch the egg cell 8;

[0062] see Figure 6 , which is a schematic diagram of the relative positions of a glass needle and an egg cell provi...

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Abstract

This application provides an operating system for egg cell microinjection, which uses the vibration of the vibration mechanism to drive the glass needle placed in the water to rotate at a high speed to cause a vortex in the water, and then moves and rotates the egg cell, and finally achieves the purpose of egg cell injection; Compared with the existing system that utilizes micro-manipulators to manipulate egg cells, this application does not make mechanical contact with egg cells, avoiding direct or indirect damage to egg cells, and the end of the glass needle can continuously rotate egg cells in situ, which is easy to move The egg cells are controlled within the field of view, which is convenient for observation; compared with the existing system of operating egg cells in the microfluidic chip, this application is not limited by the operation task and the size of the egg cells, and has strong operational flexibility.

Description

technical field [0001] The invention belongs to the technical field of micro-nano operation, and in particular relates to an operating system and method for egg cell microinjection. Background technique [0002] Cell microinjection is an important part of single cell analysis, and it is a basic way to transfer genes into cells. Cell injection is required for cloning and transgenic techniques. Among them, cell movement and cell rotation are the most important and basic operations in cell microinjection. Before and after moving cells to a certain position, they need to be rotated and oriented with multiple degrees of freedom to adjust their posture to meet the needs of the injection position. Especially for egg cells, the nuclei are located at the edge of the cell and distributed asymmetrically, so it needs to be repositioned to the observable position of the microscope focal plane during cell injection. Therefore, moving and rotating technology plays an important role in the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/00C12N15/89
CPCC12M35/00C12N15/89
Inventor 刘晓明唐小庆李鹏云陈俊男董胜男黄强新井建生
Owner BEIJING INSTITUTE OF TECHNOLOGYGY
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