2results about How to "Increase endocytosis" patented technology

The invention discloses an OX-Dex NPs preparation based on pH and spermine double cascade response, a preparation method and application, and belongs to the technical field of medicine preparation. The preparation method comprises the following steps: firstly, activating glucan by using excessive N, N '-carbonyldiimidazole, and then reacting with ethylenediamine to synthesize an amino-modified glucan intermediate; the preparation method comprises the following steps: firstly, preparing an amino-modified dextran intermediate, then connecting L-phenylalanine to the amino-modified dextran intermediate through a coupling reaction, and then self-assembling with oxaliplatin in the presence of cucurbit [8] uril and glutaraldehyde, so that the formed OX-Dex NPs preparation shows excellent cascade response characteristic to pH and spermine. Meanwhile, the OX (at) Dex NPs shows extremely low toxicity to human normal hepatocytes (LO-2) with low spermine expression and has remarkable toxicity to mouse breast cancer cells (4T1) with high spermine expression, and an efficient and safe technical scheme is provided for selection of breast cancer chemotherapy drugs.

The application provides a preparation method of an aggregation-induced emission nanoparticle, which comprises the following steps: adopting triphenylamine as an electron donor (D), pyridine bromide as an electron acceptor (A), constructing a D-π-A structure, and synthesizing a nanoscale AIE photosensitizer nanoparticle by acting with an albumin stock solution; the nanoparticle synthesis method is easy to operate, has high yield and low cost, can simultaneously generate type I and type II reactive oxygen species (ROS), including ·OH (type I) and O2 (type II), and has good mitochondrial targeting function, and a Pearson correlation coefficient is as high as 0.97. 1 In addition, due to the modification of the terminal carboxyl group of TTPA, the nanoparticle has a pH response effect, can increase the accumulation or endocytosis of the nanoparticle in tumors, and can enhance the in-vivo imaging and treatment effect, and has potential clinical application value of intelligent response cancer diagnosis and treatment function.