48results about How to "Increase endocytosis" patented technology

The invention discloses preparation and application of an SERS-fluorescence dual-mode optical imaging probe with a tumor double-targeting function. The nano-probe is composed of an imaging function core part and a targeting function shell part. The imaging function core part comprises silver nanoparticles adsorbed with Raman reporter molecules and a silicon dioxide shell layer doped with fluorescence dye, so that the probe has the SERS imaging function and the fluorescence imaging function simultaneously. The targeting function shell comprises a polyethylene glycol (PEG) coupling agent and two polypeptides specifically targeting tumor cells. According to the probe, the more effective targeting capacity is supplied to the nanoparticles by adopting a dual-polypeptide modification method, therefore, the probe can well enter the tumor cells, recognition of the probe to the tumor cells is enhanced, and SERS-fluorescence dual-mode imaging on a tumor is achieved.

The invention provides polyethyleneimine-polylysine copolymer which has the structure of a formula (I). The invention also provides a preparation method of the polyethyleneimine-polylysine copolymer. The method comprises the following steps of: carrying out polymerization reaction to the polyethyleneimine and epsilon-carbobenzoxy-L-lysineN-carboxylic acid anhydride in a first organic solvent so as to obtain polyethyleneimine poly-epsilon-carbobenzoxy-L-lysine copolymer; and mixing the halogen hydride solution, a second organic solvent and the polyethyleneimine poly-epsilon-carbobenzoxy-L-lysine copolymer, and reacting so as to obtain the polyethyleneimine-polylysine copolymer with the structure of the formula (I). The polyethyleneimine-polylysine copolymer provided by the invention is a novel polycation gene carrier, the properties of the polyethyleneimine and the polyamino acid are integrated, and thus the copolymer has relatively high transfection efficiency.

The invention teaches the use of a tricyclic compound for promoting the endocytotic uptake of macromolecular active ingredients.

The invention discloses a pH response type lipid based on dendrimers as well as a preparation method and application of the pH response type lipid, and belongs to the field of biological materials. The pH response type lipid disclosed by the invention has different surface charges under different pH environments. The specific forms are that negative electricity, neutrality or micro positive electricity are shown in an in vivo physiological environment to ensure that the pH response type lipid can have good biocompatibility, and amino groups are gradually exposed to expose more positive charges or realize charge reversal by using the pH differences between tumor tissues and normal tissues or between the inside and outside of tumor cells when the pH response type lipid reaches tumor tissue places, so that the contact between a positive electric carrier and a negative electric cell membrane is finished, and then the process of cell endocytosis is promoted. In a cytolysosome, a cationic vector continuously gives play to proton sponge effect or lysosome membrane splitting action so as to escape from lysosome.

The invention provides a drug carrier system. The drug carrier system comprises a pH sensitive shielding system, a cation carrier and electronegative drugs, wherein the pH sensitive shielding system is a copolymer of hyperbranched polyethyleneimine, lysine and glutamic acid or the copolymer of hyperbranched polyethyleneimine, lysine and aspartic acid; in the copolymer of the hyperbranched polyethyleneimine, lysine and glutamic acid, the molar ratio of the glutamic acid to the lysine is (1.5-50):1; in the copolymer of the hyperbranched polyethyleneimine, lysine and aspartic acid, the molar ratio of the aspartic acid to the lysine is (1.5-50):1; and the weight ratio of the pH sensitive shielding system to the cation carrier is (0.5-100):1. The drug carrier system has pH value responsibility and obvious difference of cytotoxicity under different pH conditions and can be used for intelligently releasing drugs.

The present invention discloses a whitening and freckle removing composition, a whitening and freckle removing cream and a preparation method thereof. The whitening and freckle removing composition disclosed by the present invention includes nicotinamide, 4-butylresorcinol, a bifida ferment lysate and a chrysanthemum extract which are in the mass ratio of (1-5):(1-3):(0.3-2):(0.4-2), and all the components are synergistic, so that the whitening and freckle removing effects are greatly improved, the efficient and comprehensive whitening and freckle removing composition is formed, comprehensive,rapid and lasting whitening and freckle removing effects on the skin may be realized, and the whitening and freckle removing effects are good. The whitening and freckle removing cream disclosed by the present invention is based on the whitening and freckle removing composition, has the comprehensive, rapid and lasting whitening and freckle removing effects and is good in stability and durable. Byusing the preparation method disclosed by the present invention, a preparation process may be controlled according to characteristics of all raw materials in the whitening and freckle removing cream,the whitening and freckle removing cream which is uniform in texture dispersion and good in stability may be prepared, and the whitening and freckle removing cream is easy to apply and capable of fitting to the skin.

The invention relates to a carbon dot used as an antitumor drug and a preparing method and application of the carbon dot. With arginine being a carbon source, arginine is completely dissolved with water, and an appropriate amount of thylenediamine is added and subjected to ultrasonic dissolving and microwave heating to obtain the target product carbon dot. Since cancer cells themselves in an organism contain hydrogen peroxide, the carbon dot can be used for treating cancer. The carbon dot can not only conduct fluorescence imaging, but also has the advantages of being high in biocompatibility,large in NO releasing amount, long in acting circular time, high in drug stability, cheap, easy to obtain and the like.

The invention belongs to the technical field of biological medicines, and particularly relates to a preparation method of a mediated-heparin intracellular delivery crosslinking nanometer carrier. PEG-modified PEI is adopted; and heparin is entrapped by using cation performance of PET, so that the cell endocytosis ability is increased; the water solubility, the stability and the blood metabolic capability of a carrier and nano composites are improved by PEG molecules; removal of combination of composites and protein is avoided; meanwhile, degradation of the heparin caused by heparin enzyme action is avoided; in addition, effective release of the heparin in cells is achieved; and a disulfide bond is introduced through a crosslinking agent on the basis, so that nanoparticles are degraded to release the heparin, thus the heparin plays a biological effect in the cells.

The invention relates to a DC targeted nano vaccine applied to immunotherapy of liver cancer and a preparation method thereof, which are characterized in that mannose modified ethylene glycol chitosanis used as a carrier material, a liver cancer specific polypeptide antigen phosphatidylinositol proteoglycan-3144-152 peptide (GPC-3144-152, FVGEFFTDV) is an immunizing antigen, and the nano vaccineis prepared by electrostatic interaction. The nano vaccine can increase the antigen endocytosis quantity of dendritic cell (DC), protect antigen from rapid degradation and clearance, prolong the action time of antigen, and stimulate the body to produce an effective immune response. The raw materials of the nano vaccine are cheap and easily available, the preparation method is simple and easy to repeat, the large-scale processing and production are simple, the immunotherapy effect of liver cancer can be enhanced, and therefore, the DC targeted nano vaccine has good application prospects.

The invention discloses a polymethacrylamide cationic polymer cooperatively modified by side groups natural arginine and lactobionic acid, a preparation method and application thereof as a gene carrier. A methacrylamide cation functional polymer with amino as a side group is synthesized through reversible addition-fragmentation chain transfer (RAFT) polymerization and subsequent deprotection reaction, then arginine containing a protective group and a lactobionic acid building block are linked to a polymethacrylamide molecule, and finally the protective group is removed for obtaining the target polymer. The preparation method of the polymethacrylamide cationic polymer is simple and mild in reaction conditions. Cell evaluation results show that the prepared polymethacrylamide cationic polymer cooperatively modified by side groups natural arginine and lactobionic acid has high biological compatibility, low cytotoxicity, high gene transfection efficiency and good serum stability, and is an effective novel glycosyl-modified non-virogene carrier, and is applicable as an efficient gene transfection reagent.

The invention discloses application of a polypeptide for preparing medicine for treating the Alzheimer disease. The amino acid sequence is shown in SEQ ID NO:1. The polypeptide is an extracellular section 41-81 amino acids of a TREM2 receptor protein, and the nucleotide sequence is shown in SEQ ID NO:2. The polypeptide has the application that the microglial mediated inflammation reaction is promoted, apoptosis of microglial cells is restrained, the migration capability of microglial cells is enhanced, Abeta endocytosis and degradation of the microglial cells are promoted, the density of microglial cells inside a brain is increased, and Abeta amyloid plaque deposition inside the brain is lowered.

The invention discloses an ultrasonic contrast agent for stem cell ultrasonic tracing and a preparation method thereof. The ultrasonic contrast agent is constructed from stem cells modified by lipid PLGA nano-micro-bubbles,wherein the complex comprises CD90-targeted lipid PLGA nano-micro-bubbles and stem cells which are interacted with each other. According to the ultrasonic contrast agent,the lipid PLGA nano-micro-bubble and stem cell complex is successfully constructed by using modified nano-micro-bubble groups and an abundantly expressed CD90 antigen molecular reaction,endocytosis of the stem cells on the lipid PLGA nano-micro-bubbles can be enhanced,the activity and dryness of the stem cells are kept,ultrasonic real-time imaging guide stem cell transplantation therapy is realized,thenenhancement of the stem cell transplantation treatment effect is further assisted,and the ultrasonic contrast agent has certain significance in the field of stem cell tracing.

The present invention relates to a delivery carrier including liposomes or nanoparticles for targeting the cells expressed with somatostatin receptor, consisting of a plurality of liposomes that have one phospholipid bilayer coating, one hydrophilic core and a bioactive substance. The bioactive substance is packaged in the hydrophilic core, or embedded in the phospholipid bilayer, or electrically bound with liposomes as a complex, wherein the phospholipid bilayer coating is conjugated with a plurality of molecules in the outer surface. The molecules recognize the somatostatin receptor in the surface of the target cells and induce receptor-mediated endocytosis.

The invention provides an anti-malignant lymphoma fusion protein. The anti-malignant lymphoma fusion protein comprises an inhibitor SARI (Suppressor of AP-1 Regulated by Interferon) of a human activator protein-1 and a mutant protein msBAFF of human B cell activating factor BAFF, wherein the msBAFF is a mutant which is formed after amino acids at the 217-224 loci of the human BAFF are replaced by two glycines. The invention further provides a preparation method of the fusion protein. After the recombinant fusion protein of the invention is obtained through a genetic engineering strategy, vitro anti-tumor effect detection is performed and shows that the tumor growth can be effectively inhibited in cells and animals.

The invention discloses a respiratory tract homing siRNA atomizing nanometer drug delivery system which uses salbutamol guanidinated chitosan treated by ultrasonic atomization as a carrier material, and is prepared by wrapping siRNA which interferes with target pathogenic genes. The invention also discloses a preparation method of the respiratory tract homing siRNA atomizing nanometer drug delivery system. The drug delivery system disclosed by the invention can be used to deliver siRNA which interferes with target pathogenic genes so as to play an RNA interference role and inhibit the replication of the pathogenic genes, can deliver siRNA with the target of lung respiratory tract epithelia in vivo, and is a non-virus delivery system which exerts corresponding drug effect by aiming at silence the target genes.

The invention discloses a dopamine ligand-containing diblock polymer, and a synthesis method and an application thereof. The polymer uses polyethylene glycol as a hydrophilic end and phenylboronic acid-modified polyethylene glycol as a targeting ligand, and uses glutamic acid and glutamic acid-dopamine as the hydrophobic end of a repeating unit. The dopamine ligand-containing diblock polymer prepared in the invention can be used for encapsulating doxorubicin to form drug-loaded nanomicelles, then iron ions are added, and the drug-loaded nanomicelles are crosslinked through coordination bonds formed between dopamine and the iron ions in order to form core-crosslinked doxorubicin-loaded nanomicelles. The core-crosslinked micelles prepared in the invention has the advantages of simple preparation method, high drug encapsulation rate, keeping stable during blood circulation, and realization of the release of pH-sensitive drugs in tumor cell lysosomes.

The invention provides a mixed medicament carrying micelle based on polyphosphoester, a preparation method thereof and a mixed medicament carrying micelle modified by a positive targeted group. A compound micelle consists of polyphosphoester-polylactic acid two segmented copolymers and polyethylene glycol-polylactic acid two segmented copolymers; the compound micelle with the same polylactic acid hydrophobic kernel and different hydrophilic shells is formed under the hydrophobic interaction of polylactic acid segments. The mixed medicament carrying micelle provided by the invention has the advantages of high hydrophilicity and high stability, is easy to perform targeted modification, has an in vivo long circulation effect, effects of facilitating of targeted cell endocytosis and in-cell medicament release, targeted modified mixed medicament carrying micelle can achieve a passive targeting effect and a positive targeting effect, so that the tumor treatment effect is further improved.

The invention discloses an acid environment sensitive nano predrug system with a cellulose nanocrystal used as a carrier and a preparation method. The predrug system comprises a rodlike cellulose nanocrystal, a connection unit and a drug, wherein the rodlike cellulose nanocrystal is the carrier, and the acid environment sensitive connection unit is connected with the drug; the system is of a structure shown in a formula I. A preparation process of the acid environment sensitive nano predrug system comprises the following steps: (1) preparing an aminated cellulose nanocrystal; (2) performing ring-opening reaction on the drug and cis-acotonic anhydride (CAA); (3) preparing the acid environment sensitive nano predrug system with the cellulose nanocrystal used as the carrier through heterogeneous reaction. The predrug system prepared through the preparation method disclosed by the invention realizes controlled release of the drug sensitive to a micro-acid environment at a nidus, and has relatively ideal capacities of endocytosis and tumor cell proliferation inhibition.

The invention relates to a preparation method of an antigen and adjuvant co-delivery nano-vaccine applied to liver cancer immunotherapy. The preparation method comprises the steps as follows: step 1,mixing a GPC3127-136 aqueous solution and a polyethylenimine (PEI) aqueous solution to obtain GPC3127-136 and PEI mixed solution, and mixing a sodium alginate aqueous solution and a CpG aqueous solution to obtain a sodium alginate and CpG mixed solution; and step 2, dropwise adding the sodium alginate and CpG mixed solution to the GPC3127-136 and PEI mixed solution under the stirring condition, and performing stirring continuously to obtain the antigen and adjuvant co-delivery nano-vaccine applied to liver cancer immunotherapy.