4results about How to "Increase endocytosis" patented technology

The invention belongs to the technical field of medical materials, discloses hyaluronic acid modified phosphorus-doped double-copper monatomic nano-enzyme (Cu2 / PNC at HA) and a preparation method and application thereof, and aims to solve the problems of low catalytic efficiency and poor selectivity of nano-enzyme. The preparation method comprises the following steps: firstly synthesizing a ZIF-8 carrier, then coating double copper ions and poly (cyclotriphosphoryl-4 ', 4'-diaminodiphenyl ether) (PZM) on the surface of the ZIF-8 through polymerization, and further performing pyrolysis to obtain the Cu2 / PNC nano-enzyme. The catalytic efficiency of the Cu2 / PNC nano-enzyme is remarkably improved through the phosphorus doping effect and double copper single atoms. The hyaluronic acid is adopted to coat the Cu2 / PNC nano-enzyme, so that the biocompatibility is improved, the anti-tumor activity is remarkably improved, the application of the Cu2 / PNC nano-enzyme in the field of biomedicine is expanded, and the huge potential in the aspect of tumor catalytic treatment is shown.

The invention provides a whole tumor cell vaccine as well as a preparation method and application thereof, and relates to the technical field of cancer immunotherapy. The preparation method of the whole tumor cell vaccine provided by the invention comprises the following steps: obtaining CD47 gene knockout tumor cells, inserting an adjuvant into the surfaces of the tumor cells, and carrying out heating inactivation treatment to prepare the whole tumor cell vaccine, the temperature of the heating inactivation treatment is 40-80 DEG C. According to the preparation method, CD47 genes of tumor cells are knocked out, so that endocytosis of macrophages to vaccines is enhanced; an adjuvant is inserted into the surface of the tumor cell after the CD47 gene is knocked out, so that the immune activation effect of the vaccine is enhanced; the cells are inactivated by heating and inactivating treatment at a proper temperature, so that the safety of the vaccine is ensured, and meanwhile, the cells which die due to immunogenicity can also have a stronger immune activation effect. The preparation method is simple and convenient, and the prepared whole tumor cell vaccine can be used for preventing and treating tumors.

The invention discloses an OX-Dex NPs preparation based on pH and spermine double cascade response, a preparation method and application, and belongs to the technical field of medicine preparation. The preparation method comprises the following steps: firstly, activating glucan by using excessive N, N '-carbonyldiimidazole, and then reacting with ethylenediamine to synthesize an amino-modified glucan intermediate; the preparation method comprises the following steps: firstly, preparing an amino-modified dextran intermediate, then connecting L-phenylalanine to the amino-modified dextran intermediate through a coupling reaction, and then self-assembling with oxaliplatin in the presence of cucurbit [8] uril and glutaraldehyde, so that the formed OX-Dex NPs preparation shows excellent cascade response characteristic to pH and spermine. Meanwhile, the OX (at) Dex NPs shows extremely low toxicity to human normal hepatocytes (LO-2) with low spermine expression and has remarkable toxicity to mouse breast cancer cells (4T1) with high spermine expression, and an efficient and safe technical scheme is provided for selection of breast cancer chemotherapy drugs.

The application provides a preparation method of an aggregation-induced emission nanoparticle, which comprises the following steps: adopting triphenylamine as an electron donor (D), pyridine bromide as an electron acceptor (A), constructing a D-π-A structure, and synthesizing a nanoscale AIE photosensitizer nanoparticle by acting with an albumin stock solution; the nanoparticle synthesis method is easy to operate, has high yield and low cost, can simultaneously generate type I and type II reactive oxygen species (ROS), including ·OH (type I) and O2 (type II), and has good mitochondrial targeting function, and a Pearson correlation coefficient is as high as 0.97. 1 In addition, due to the modification of the terminal carboxyl group of TTPA, the nanoparticle has a pH response effect, can increase the accumulation or endocytosis of the nanoparticle in tumors, and can enhance the in-vivo imaging and treatment effect, and has potential clinical application value of intelligent response cancer diagnosis and treatment function.