New application of curcumin

A technology of curcumin and medicine, applied in the field of curcumin, can solve problems such as unproven effects

Inactive Publication Date: 2011-04-20
PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Animal experiments have confirmed that the application of angiotensin-converting enzyme inhibitors (angiotensin-converting enzyme inhibitors, ACEI) or angiotensin receptor blocker (angiotensin receptor blocker, ARB) to control blood pressure may delay p

Method used

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  • New application of curcumin
  • New application of curcumin
  • New application of curcumin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0081] Embodiment 1, curcumin are to the inhibitory action of MDCK vesicle formation and growth

[0082] 1. Curcumin can inhibit the formation of vesicles

[0083] Canine kidney cells (MDCK) were stimulated by cAMP to form vesicles when cultured in three-dimensional Matrigel, and they continued to grow. Forskolin is an activator of adenylyl cyclase, which can mediate the generation of cAMP, so forskolin can promote the formation and growth of MDCK vesicles, whose vesicle properties are similar to those of polycystic kidney vesicles, It is the best in vitro model for screening and evaluating the pharmacological activity of compounds for treating polycystic kidney disease.

[0084] MDCK cells (purchased from ATCC, USA, catalog number CCL-34) were cultured in culture media containing 10 μM forskolin (Forskolin, purchased from Sigma, USA, catalog number F6886) and concentrations of 0 M, 4×10 -7 M, 2×10 -6 M and 1×10 -5 In the three-dimensional Matrigel (Purecol Collagen, purch...

Embodiment 2

[0089] Embodiment 2, the cytotoxicity of curcumin, the impact on cell growth and differentiation

[0090] 1. Determine the cytotoxicity of curcumin by MTT assay

[0091] The log phase MDCK cell suspension was inoculated in a 96-well culture plate, and each well contained 4×10 3 Each well was given 200 μl of MDCK cell culture medium (made from DMEM medium (purchased from U.S. Invitrogen Company, catalog number 12100-046) and F12 medium (purchased from U.S. Invitrogen Company, catalog No. 21700-075) in equal volumes. mixed), placed in 5% CO at 37°C 2 Incubate for 24 hours in the incubator. Then add the final concentration of 1 × 10 to the cell culture plate -4 M, 1×10 -5 M, 1×10 -6 M and 1×10 -7 Curcumin of M, continue to cultivate for 24 hours. Remove the supernatant, add 200 μl MDCK cell culture medium and 20 μl MTT solution with a concentration of 5 mg / ml, and continue to cultivate for 3 hours. Remove the supernatant, add 150 μl dimethyl sulfoxide to each well, shake ...

Embodiment 3

[0098] Embodiment 3, determine the inhibitory effect of curcumin on embryonic kidney vesicle growth by in vitro embryonic kidney model

[0099] On the afternoon of the first day, 6-week-old ICR mice (purchased from the Experimental Animal Center of Peking University Health Science Center) were mated in the same cage at a ratio of 1:1. It means that the female mice have been pregnant for half a day, and the mice without vaginal plugs are divided into cages first, and then re-caged in the afternoon, and then observed on the second day; the pregnant female mice continue to be fed alone for 13 days, and the embryonic kidneys are taken on the 13th day and cultured on a transwell plate.

[0100] Take the mouse embryonic kidneys at day 13.5 above, and under the action of 100 μM 8-Br-cAMP (purchased from Sigma, USA, catalog number B-1381), multiple and progressively growing renal vesicles are formed in the kidney tissue. It can be used as an in vitro whole organ model to evaluate the ...

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Abstract

The invention discloses application of curcumin to preparation of medicaments for preventing and/or treating autosomal dominant polycystic kidney disease. A madin-darby canine kidney (MDCK) vesicle model is used for screening to obtain the curcumin which inhibits formation and growth of vesicles. An experimental result shows that: the curcumin has an obvious inhibiting effect on the formation and growth of MDCK vesicles and the effect of the curcumin is in dose response relationship; the curcumin has no cytotoxicity to MDCK cells, so that the vesicle inhibiting effect of the curcumin is independent of the cytotoxicity; the curcumin does not obviously induce MDCK cell apoptosis, so that the vesicle inhibiting effect of the curcumin is independent of cell apoptosis promotion of the curcumin; the curcumin can promote the MDCK cells or vesicles to form tubular structures; and the effect is in dose response relationship; and the curcumin has an inhibiting effect on the growth of the embryonic kidney vesicles. The curcumin is expected to be developed into a specific medicament for preventing and/or treating autosomal dominant polycystic kidney disease.

Description

technical field [0001] The present invention relates to a new application of curcumin. Background technique [0002] 1. The characteristics and pharmacological effects of curcumin [0003] 1. General characteristics of curcumin [0004] Curcumin (Curcumin, Cur) is a natural polyphenolic compound extracted from the rhizomes of Curcuma longa, Curcuma longa, Curcuma longa, etc. (its chemical structure is as follows: figure 1 shown). Curcumin is easily soluble in methanol, ethanol, alkali, acetic acid, acetone, dimethyl sulfoxide and other organic solvents, almost insoluble in water, and its molecular formula is C 21 h 20 o 6 , the molecular weight is 368.38, and its main chain is unsaturated aliphatic and aromatic groups. Curcumin has various pharmacological effects such as anti-oxidation, anti-fibrosis, anti-tumor, anti-depression, anti-inflammatory, anti-viral, anti-thrombotic, anti-angiogenic and immune regulation, and has low toxicity. However, the blood concentratio...

Claims

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Application Information

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IPC IPC(8): A61K31/12A61P13/12
Inventor 杨宝学周虹高晋生雷天落
Owner PEKING UNIV
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