Production method for influenza virus vaccines

Abstract

The invention discloses a production method for vaccines of avian influenza virus and other influenza virus such as swine influenza, dog influenza and equine influenza, which comprises the following steps of: (1) transfer of culture and cultivation of vaccine-made cells; (2) reproduction of vaccine-made virus seeds; (3) microcarrier suspension culture of MDCK cells in a bioreactor; (4) reproduction of vaccine-made virus liquid; and (5) harvest of the virus liquid. The production method has the advantages of reducing the production cost greatly and improving the yield and quality of the vaccines obviously, along with short production period, no restriction to raw material supply, small occupied area, easy and quick expansion of production scale, light environmental pollution, easy processing, high automaticity, low employment and easy realization of balanced and stable quality.

Description

technical field [0001] The invention relates to a method for producing influenza vaccines of avian influenza virus and other influenza viruses such as swine influenza, canine influenza and equine influenza by using bioreactor microcarrier cell culture. It can be used for industrial production of influenza vaccines for avian influenza virus and other influenza viruses such as swine influenza, canine influenza, and equine influenza, replacing the traditional chicken embryo culture method. Background technique [0002] At present, the production of influenza vaccines for domestic avian influenza virus and other influenza viruses such as swine influenza, canine influenza, and equine influenza only relies on the chicken embryo method. The traditional process is labor-intensive, time-consuming, low in efficiency, and high in production cost; it is easily polluted by the environment; the difference between different batches of chicken embryos is large; it is difficult to expand pro...

AI Technical Summary

Problems solved by technology

The traditional process is labor-intensive, time-consuming, low in efficiency, and high in production cost; it is easily polluted by the environment; the difference between different batches of chicken embryos is large; it is difficult to expand production; the chicken embryo itself is contaminated by bacteria or other viruses to produce vaccines There may be quality hazards; the number of waste embryos is large, and it is difficult to harmlessly dispose of them, which involves biosafety and public health issues

In addition, there have been reports on the use of bioreactor microcarriers to cultivate rabies vaccines, but there have been no reports on the use of bioreactor microcarriers to produce influenza vaccines for avian influenza viruses and other influenza viruses such as swine influenza, canine influenza, and equine influenza

Images