Medicinal use of a kind of astragaloside compound
A technology of astragaloside IV and its application, which is applied in the field of medicinal use of astragaloside IV compounds, and can solve the problems such as difficulty in preventing and treating influenza.
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Embodiment 1
[0030] Embodiment 1 Astragaloside IV (AST) is to cytotoxicity assay experiment
[0031] When astragaloside IV AST was applied to MDCK cells at concentrations of 5 μg / ml, 10 μg / ml, 20 μg / ml, 40 μg / ml, 60 μg / ml, 80 μg / ml, and 100 μg / ml for 72 hours, MDCK cells were treated with 80 μg of astragaloside AST. When the concentration is 100μg / ml, there is some rounding under the light microscope, but there is no obvious toxicity, and when the concentration is 100μg / ml, there is obvious cell rounding. When the concentration is higher than 100μg / ml, the cytotoxicity is more obvious, and there is obvious cell aggregation phenomenon. The OD value of each hole was measured by MTT method, and the results showed that: when the concentration of MDCK cells was 100 μg / ml, the survival rate was more than 93%, while at 200 μg / ml, the survival rate was reduced to 30%, and when it exceeded 200 μg / ml, the cells were almost inactive ( Such as figure 1 shown). This shows that astragaloside AST has ...
Embodiment 2
[0032] Embodiment 2 Astragaloside IV (abbreviated as AST) to the inhibition experiment of influenza virus
[0033] 1. Drugs and reagents
[0034] Astragaloside IV (Astragaloside IV), measured by HPLC with a purity of ≥98%, was purchased from AMA Copoeia Reference (40mg), and was dissolved in DMSO when used; fetal bovine serum (FBS), tetramethylthiazolium blue (MTT), bovine serum Albumin (BSA) and dimethyl sulfoxide (DMSO) were purchased from Sigma, USA; trypsin-EDTA, penicillin, and streptomycin solutions were purchased from Gibco; secondary antibodies 488-labeled goat anti-mouse IgG (H+L) was purchased from Cell Signaling Technology; DAPI nuclei luminescent dye was purchased from Beyond Biotech Co., Ltd.
[0035] 2. Viruses and Cells
[0036]The throat swab samples collected from influenza-like cases were subjected to nucleic acid and virus isolation, detection and typing, and one-step RT-PCR method was used to amplify the HA gene and NA gene of a selected strain of influe...
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