Joint detection kit for novel influenza virus and influenza virus A/B as well as preparation method and application of joint detection kit

Abstract

The invention discloses a joint detection kit for novel influenza virus and influenza virus A / B as well as preparation method and application of the joint detection kit. The joint detection kit comprises a detection test strip, the detection test strip comprises a sample pad, a combination pad, a detection pad and an absorption pad which are sequentially connected in an overlapped mode, and the detection pad is provided with a detection line and a quality control line which are parallel to each other; the detection line comprises a first detection line, a second detection line and a third detection line which are separated from one another, the first detection line is coated with a new coronavirus antibody, the second detection line is coated with an influenza A virus antibody, and the third detection line is coated with an influenza B virus antibody. The joint detection kit provided by the invention can simultaneously detect three items of the new coronavirus, the influenza A and the influenza B after one-time sampling treatment, is short in detection time and simple to operate, and avoids missed diagnosis or misdiagnosis in single item detection; besides, the repeated contact between medical staff and patients in multiple sample collection processes is reduced, and the risk of epidemic situation spreading is reduced.

Description

technical field [0001] The invention belongs to the technical field of medical testing, and in particular relates to a novel coronavirus and influenza A and B influenza virus antigen joint detection kit and its preparation method and application. Background technique [0002] The 2019 novel coronavirus (2019 Novel Coronavirus, 2019-nCoV) is an RNA virus, belonging to the genus β coronavirus, with an envelope, and the particles are round or oval, often pleomorphic, with a diameter of 60-140nm. The main clinical manifestations of 2019-nCoV infection are fever, dry cough, and fatigue. A small number of patients have symptoms such as nasal congestion, runny nose, sore throat, myalgia, and diarrhea. Severe patients often develop dyspnea or hypoxemia one week after the onset, and severe cases can rapidly progress to acute respiratory distress syndrome, septic shock, difficult-to-correct metabolic acidosis, coagulation dysfunction, and multiple organ failure Wait. [0003] Influ...

AI Technical Summary

Problems solved by technology

[0005] At present, most of the existing detection products (kits) on the market can only be used for a single detection of new crown or influenza, and the respective sample extracts cannot replace each other. When the clinical needs of simultaneous detection of the two are usually repeated sampling, and use the corresponding kits of the respective reagents Repeated processing of samples with sample extraction solution is cumbersome, and there is a risk of exposure, which increases the burden on patients and the risk of infection for medical staff

[0006] The Chinese invention patent with the authorized announcement number CN 111579792B discloses a joint detection device for influenza virus and 2019 new coronavirus antibody and its preparation method, which can detect influenza and new coronavirus IgM antibody at the same time. However, under normal circumstances, the virus infects the body to produce IgM antibody takes a certain amount of time, so there is a certain "window period" for the detection of IgM antibody. For some patients who have recently been infected and have not yet produced antibodies, it is easy to miss the detection. In addition, antibody detection requires blood drawing, and there are professional requirements for sampling personnel, and Time-consuming and labor-intensive, increasing exposure risk

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