Reference gene for respiratory tract RNA virus PCR detection and detection product thereof

An RNA virus and internal reference gene technology, applied in the field of internal reference genes and their detection products, can solve problems such as false negatives, and achieve the effect of improving the accuracy of diagnosis
CN111363848APending Publication Date: 2020-07-03SHANGHAI SHEN LIAN BIOMEDICAL CORP
5 Cites 2 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SHANGHAI SHEN LIAN BIOMEDICAL CORP
Publication Date
2020-07-03

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention provides a reference gene for respiratory tract RNA virus PCR detection and a detection product thereof. The reference gene is a DDX5 gene. The invention also designs a primer and a probe aiming at the reference gene. Compared with a reference gene RNase P (RPP30) for American CDC and corresponding primers and probes, the primers and the probes which are designed and screened according to the invention and aim at the reference gene are highly specific, only mRNA is amplified, and genomic DNA is not amplified; and the reference gene can be stably detected in people.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention relates to the technical field of respiratory RNA virus detection, in particular to an internal reference gene for PCR detection of respiratory RNA virus and a detection product thereof. Background technique

[0002] Respiratory virus infection is one of the leading causes of death and disability worldwide. According to statistics, the influenza virus killed more than 100 million people in the last century [Eric T Beck, 2010]; among children under 5 years old, acute lower respiratory tract infection caused more than 20% of the deaths, among which pneumonia was the most serious, accounting for more than 90% [Jeanne Carr, 2010]. In addition, new respiratory viruses have appeared frequently in this century, such as severe acute respiratory syndrome virus (SARS virus, 2002), Middle East respiratory syndrome virus (MERS virus, 2012), new coronavirus (SARS-COV2 virus, 2019) , seriously endangering human life and causing heavy social and econo...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More