Novel coronavirus 2019-nCoV real-time fluorescent PCR detection primer and probe, kit and method
A technology for 2019-ncov and coronavirus, which is applied in the direction of biochemical equipment and methods, microbial measurement/inspection, and resistance to vector-borne diseases. The effect of high degree and high spiritual divergence
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[0032] Example 1: Development of a real-time fluorescent PCR detection kit.
[0033] 1. In this example, the novel coronavirus 2019-nCoV-specific coding ORF1ab and N gene sequences were selected to design two pairs of specific primers and two specific fluorescent probes, and the internal standard gene Rnase P was selected to design a pair of specific The new coronavirus 2019-nCoV can be detected by real-time fluorescent PCR technology. ORF1ab and N genes are important structural genes of the new coronavirus, and the primers and probes designed in the present invention are only 100% similar to the ORF1ab and N genes of the new coronavirus 2019-nCoV, while other coronaviruses including SARS, MERS, 229E, OC43, NL63 and HKU1 do not match, so they can specifically bind to the ORF1ab and N genes of the new coronavirus 2019-nCoV and initiate amplification, but not to amplify other types of coronavirus. The primer sequences and probe sequences designed in this embodiment of the prese...
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