Novel coronavirus 2019-nCoV real-time fluorescent PCR detection primer and probe, kit and method

Abstract

The invention discloses a novel coronavirus 2019-nCoV real-time fluorescent PCR detection primer and probe, kit and method. Two loci of nucleic acid after 2019-nCoV replication are selected for detection; the two loci include a 2019-nCoV specific locus with high specificity, and the locus has no cross reaction with other common pathogens of which infection site are the same as the locus or have similar infection symptoms and the total nucleic acid of human leukocytes. A single tube with three fluorescence channels are adopted to simultaneously detect the presence of novel coronavirus 2019-nCoVand an internal reference gene Rnase P, and can also detect the presence of the RNA of the novel coronavirus 2019-nCoV in specimens such as bronchoalveolar lavage fluid, nose swabs and throat swabs.Through the primer, probe, kit and method, detection time is short and can be shortened to 1.5 h, so that the primer, probe, kit and method are suitable for rapid clinical and bedside detection and diagnosis; and the primer, probe, kit and method are high in detection of virus specificity, and high in repeatability, sensibility and accuracy rate.

Description

technical field [0001] The invention belongs to the technical field of virus detection, and in particular relates to a novel coronavirus 2019-nCoV real-time fluorescent PCR detection primer and probe, a kit and a method. Background technique [0002] 2019-nCoV (novel coronavirus) belongs to a newly discovered coronavirus, which belongs to the same family as SARS. This virus is round or oval, granular, and its diameter is about 60-140nm. The infectivity of this virus is relatively Strong, there is no specific detection method at present, and it is common for this virus to cause severe respiratory infection in humans, and confirmed patients need to be treated in isolation, so it is urgent to develop a method that can quickly and accurately detect this new type of coronavirus 2019-nCoV The identified molecular method can effectively control the spread of the epidemic, and the purpose of rapid diagnosis, isolation and treatment of patients can be achieved. Contents of the inve...

AI Technical Summary

Problems solved by technology

[0002] 2019-nCoV (novel coronavirus) belongs to a newly discovered coronavirus, which belongs to the same family as SARS. This virus is round or oval, granular, and its diameter is about 60-140nm. The infectivity of this virus is relatively Strong, there is no specific detection method at present, and it is common for this virus to cause severe respirat...

Images