Exterior guiding gene C6 concurrently used with RnaseP ribozyme and its uses

Abstract

This invention discloses an external guiding gene and its application, wherein the gene is used combining with the RNase P ribozyme. The related external guiding sequence is based on the RNA, which has the sequence of the SEQ ID NO: 1, and it also includes the external guiding sequence based on the DNA, which has the sequence of the SEQ ID NO: 2, all of the sequences has the special guiding cutting activity that guides RNase P to cut HCMV polyenzyme mRNA flake, and the target point is at 1760-1776 of the UL54 gene. The EGS in this invention is to research and exploit the HCMV infection disease treatment and the gene drugs of related disease.

Description

technical field [0001] The invention belongs to the field of molecular biology and medicine, and in particular relates to an external guide sequence combined with RNase P ribozyme and its application in preparing anti-HCMV medicines. Background technique [0002] In the 1980s, Sidney Altman et al. found that the RNA component M1 RNA of Escherichia coli ribonuclease P (RNase P) can independently cut and process the precursor tRNA (ptRNA). RNA with catalytic cleavage activity is called "ribozyme". The discovery of ribozymes broadened the horizons of gene therapy. The complementary cleavage and recycling properties of ribozymes have obvious advantages in gene therapy of mammalian cells. Experimental results in recent years have shown that ribozymes, as a tool for gene therapy, have broad application prospects and great clinical practical value. [0003] RNase P is a nucleoprotein complex that widely exists in bacteria, yeast, animals and plants. It is a natural ribozyme poss...

AI Technical Summary

Problems solved by technology

In fact, since many mRNAs in the cell are combined with proteins and exist in a highly organized and folded spatial structure, under laboratory conditions where accurate analysis of the secondary structure region of the substrate RNA is not yet possible , it is extremely difficult to select a target site on the substrate RNA that is easy to bind to EGS

Even if you choose a target with high cleavage activity in the M1GS experiment, under the influence of other factors, it may not be able to achieve efficient guided cleavage in the EGS experiment

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