Mycoplasma genitalium nucleic acid real-time fluorescence PCR detection primer, probe and kit

Abstract

The invention discloses a mycoplasma genitalium nucleic acid real-time fluorescence PCR detection primer, a probe and a kit. The kit comprises an amplification reaction solution, a mycoplasma genitalium nucleic acid detection solution, a positive reference substance and a negative reference substance, wherein the mycoplasma genitalium nucleic acid detection liquid comprises specific primers and probes aiming at mycoplasma genitalium and a human RNase P gene, the sequences of the primers aiming at the mycoplasma genitalium are as shown in SEQ ID NO.1 and SEQ ID NO.2, and the sequence of the probe aiming at the mycoplasma genitalium is as shown in SEQ ID NO.3. According to the primer, the probe and the kit for real-time fluorescence PCR detection of the mycoplasma genitalium, provided by the invention, the mycoplasma genitalium can be rapidly, accurately and sensitively detected, the repeatability of an experimental result is good, the precision is high, the detection time period is short, and the detection can be completed within 70 minutes as short as possible. Therefore, the detection time is greatly saved, and the clinical diagnosis efficiency can be accelerated.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a real-time fluorescent PCR detection primer, probe and kit for mycoplasma genitalium nucleic acid. Background technique [0002] Mycoplasma genitalium (Mycoplasma genitalium, MG) belongs to Mollusca class, Mycoplasma order, Mycoplasma family, its genome size is 580kb, is the smallest known self-replicating bacteria, no cell wall, Gram-negative. MG is one of the pathogens that cause sexually transmitted diseases, causing non-gonococcal urethritis (NGU) in men, cervicitis, endometritis and pelvic inflammatory disease in women, and can lead to ectopic pregnancy, Infertility and other adverse outcomes, and the infection of Mycoplasma genitalium may promote the transmission and development of HIV virus. Mycoplasma genitalium has very important clinical significance. [0003] The 2018 British Guidelines for the Treatment of Mycoplasma Genitalium Infection pointed out th...

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Problems solved by technology

In 2016, the "Expert Consensus on the Diagnosis and Treatment of Mycoplasma Genitalium Infection" pointed out: In terms of serology, Mycoplasma genitalium and Mycoplasma pneumoniae have many cross-reactive antigenic determinants, although various serological testing methods such as agglutination reaction and complement fixation can be used to detect Serological changes of Mycoplasma genitalium, but because Mycoplasma pneumoniae may appear in the genitourinary tract, false positives may occur in the detection of MG, and it is not practical in clinical work

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