Test strip and kit for detecting HIV antibody

A technology of test strips and kits, applied in the field of test strips and kits for detecting HIV antibodies, can solve the problem of difficulty in achieving accurate detection of oral mucosal exudate, limiting the widespread use of immunochromatographic test strips, and weak positive detection. The results are not obvious and other problems, to achieve the effect of reducing non-specific binding, enhancing sensitivity, and enhancing hydrophilicity

Inactive Publication Date: 2018-04-27
德诺杰亿(北京)生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, existing immunochromatographic test strips still have certain defects when detecting HIV antibodies, which limit the widespread use of immunochromatographic test strips, including: (1) detection sensitivity is not high, weak positive test results are not obvious, and prone to False negative; (2) Most of the current HIV antibody test strips can only detect blood samples, and it is difficult to accurately detect viscous liquid samples such as oral mucosal exudate

Method used

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  • Test strip and kit for detecting HIV antibody
  • Test strip and kit for detecting HIV antibody
  • Test strip and kit for detecting HIV antibody

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] A method for preparing test strips and corresponding kits for detecting HIV1 / 2 antibodies, comprising:

[0059] (1) Prepare 1L sample pad treatment solution according to the following formula: 0.01M PBS (pH7.4), 6g polyethylene glycol 20000, 5g casein, 3mL Tween-20, 6g polyvinylpyrrolidone K-30;

[0060] (2) Prepare 1L sample diluent according to the following formula: 2.9g disodium hydrogen phosphate dodecahydrate, 0.3g sodium dihydrogen phosphate dihydrate, 1.7g sodium chloride, 10g casein, 1g polyethylene glycol 20000, Tween -20 0.2mL, Proclin-300 1ml, the balance is purified water;

[0061] (3) Use the aforementioned sample pad treatment liquid to treat the sample pads: each 31cm×1.9cm sample pad is evenly coated with 3.2ml of the above-mentioned treatment solution, and then placed in an electric blast drying oven to dry for more than 8 hours, dry and store for later use ;

[0062] (4) Colloidal gold-labeled mouse anti-human IgG antibody on glass cellulose membran...

Embodiment 2

[0067] With reference to the method described in Example 1, prepare the test strip for detecting HIV1 / 2 antibodies and the method of the corresponding test kit, the difference is only in:

[0068] The formula for preparing 1L sample pad treatment solution is: 0.01M PBS (pH7.4), 5g polyethylene glycol 20000, 10g casein, 10mL Tween-20, 10g polyvinylpyrrolidone K-30;

[0069] The formula for preparing 1L sample diluent is: 2.9g disodium hydrogen phosphate dodecahydrate, 0.3g sodium dihydrogen phosphate dihydrate, 1.7g sodium chloride, 5g casein, 0.6g polyethylene glycol 20000, Tween-20 0.1mL, Proclin-300 0.5ml, the balance is purified water;

[0070] The coating amount of the colloidal gold-labeled mouse anti-human IgG antibody is 0.5 μg / cm 2 The concentration of the gp41 is 4.0mg / ml; the concentration of the gp36 is 0.4mg / ml; the goat anti-mouse IgG antibody concentration is 0.3mg / ml.

Embodiment 3

[0072] With reference to the method described in Example 1, the method for preparing test strips and corresponding kits for detecting HIV1 / 2 antibodies, the difference is only that,

[0073] The formula for preparing 1L sample pad treatment solution is: 0.01M PBS (pH7.4), 10g polyethylene glycol 20000, 7g casein, 7mL Tween-20, 8g polyvinylpyrrolidone K-30;

[0074] The formula for preparing 1L sample diluent is: 2.9g disodium hydrogen phosphate dodecahydrate, 0.3g sodium dihydrogen phosphate dihydrate, 1.7g sodium chloride, 12g casein, 0.8g polyethylene glycol 20000, Tween-20 0.4mL, Proclin-3001.5ml, the balance is purified water;

[0075] The coating amount of the colloidal gold-labeled mouse anti-human IgG antibody is 0.9 μg / cm 2The concentration of the gp41 is 2.0mg / ml; the concentration of the gp36 is 0.6mg / ml; the goat anti-mouse IgG antibody concentration is 0.5mg / ml.

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Abstract

The invention relates to a test strip and kit for detecting a HIV antibody. The test strip of the invention comprises a base plate and further comprises a sample pad, a marker binding pad, a nitrocellulose membrane and a water absorbing pad which are all pasted onto the base plate and sequentially overlapped, wherein the sample pad is treated with a sample pad treating fluid; the sample pad treating fluid comprises: a PBS buffer with a pH value of 7.2-7.5, polyethylene glycol with a mass fraction of 0.5-1%, casein with a mass fraction of 0.5-1%, Tween-20 with a volume fraction of 0.3 to 1% andpolyvinylpyrrolidone K-30 with a mass fraction of 0.6 to 1%; the marker binding pad is coated with an anti-human IgG antibody labeled by a marker; the nitrocellulose membrane is provided with a testline and a control line, the test line is coated with an HIV antigen, and the control line is coated with an anti-antibody; and the anti-antibody binds to the anti-human IgG antibody. The test strip of the invention can amplify the weakly positive detection result of the HIV antibody so as to reduce false negative and improve detection accuracy.

Description

technical field [0001] The invention relates to the field of immunochromatographic test strips, in particular to a test strip and a test kit for detecting HIV antibodies. Background technique [0002] AIDS, Acquired Immunodeficiency Syndrome (AIDS), is an infectious disease that seriously endangers human health caused by the Human Immunodeficiency Virus (HIV). Since the first AIDS case was reported in the world in 1981, in just 30 years, AIDS has ravaged the world and killed more than 25 million people. According to the annual report released by the United Nations Program on AIDS, in 2010, there were about 34 million HIV carriers in the world, an increase of 700,000 compared with 2009. The report shows that in 2010, there were approximately 2.7 million new HIV carriers worldwide, a decrease of 15% from 2001 and a decrease of 21% from the peak in 1997. Because this disease is still incurable at present, also does not have effective vaccine, therefore take effective measures...

Claims

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Application Information

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IPC IPC(8): G01N33/569
CPCG01N33/56988
Inventor 吴丽金张芳刘冬冉曹健荣
Owner 德诺杰亿(北京)生物科技有限公司
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