Anti-type a foot-and-mouth disease virus neutralizing monoclonal antibody and its application

A technology of foot-and-mouth disease virus and monoclonal antibody, which is applied to the preparation and application of type A foot-and-mouth disease virus monoclonal antibody, and the application in the prevention or treatment of type A foot-and-mouth disease. In the field of diagnosis, monoclonal antibodies can solve antigenic differences and diagnosis of foot-and-mouth disease and the difficulty of controlling
CN105859880BActive Publication Date: 2019-07-23HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
1 Cites 0 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
Publication Date
2019-07-23

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention discloses preparation and application of a monoclonal antibody of an A-type foot and mouth disease virus (FMDV) and belongs to the field of prevention and treatment of foot and mouth diseases. According to the preparation and the application, a hybridoma cell line secreting the neutralizing monoclonal antibody of the A-type FMDV is constructed, the microbiological collection number of the hybridoma cell line is CGMCC 12049, and the secreted monoclonal antibody is an A-type FMDV serotype specific monoclonal antibody and has high-strength neutralizing ability; and a detection method for the A-type FMDV antibody is created by using the monoclonal antibody. The monoclonal antibody prepared by the invention shows good competitive ability with strongly-positive, weakly-positive and suspected serum of the A-type FMDV in the detection method for the A-type FMDV antibody and is free of competitiveness with strongly-positive serum of an O-type FMDV, strongly-positive serum of an Asia1-type FMDV and negative serum of an FMDV, so that the monoclonal antibody can be used for specifically discriminating the negative and positive serum of the A-type FMDV and has an important application prospect in aspects such as the diagnosis, prevention and treatment of the A-type foot and mouth diseases.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The present invention relates to a monoclonal antibody, in particular to a neutralizing monoclonal antibody against type A foot-and-mouth disease virus and a hybridoma cell line secreting the neutralizing monoclonal antibody. The present invention further relates to the use of the monoclonal antibody in diagnosis and prevention Or the application in treating type A foot-and-mouth disease, belonging to the field of preparation and application of type A foot-and-mouth disease virus monoclonal antibody. Background technique

[0002] Foot and Mouth Disease Virus (Foot and Mouth Disease Virus, FMDV) belongs to Picornaviridae, Foot and Mouth Disease Virus genus, FMDV genome RNA length is about 8.5kb. Foot and mouth disease (FMD) is caused by FMD virus (FMDV), and is a highly contagious infectious disease that mainly affects cattle, pigs, sheep and other domestic animals and a variety of wild cloven-hoofed animals. The outbreak and prevalence of this disease...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More