Kit for detecting antibody against Peste des petits ruminants virus b-ELISA and preparation method thereof

Abstract

The invention relates to the technical field of biology, particularly the field of viral antibody detection. A kit for detecting the antibody against Peste des petits ruminants virus b-ELISA comprises the following ingredients which are arranged respectively: Peste des petits ruminants nucleoprotein antigen, Peste des petits ruminants monoclonal antibody, diluent, strong positive serum, weak positive serum, negative serum, HRP sheep anti-mouse secondary antibody, 20 times the concentration of washing liquid, substrate liquid, stopping solution and enzyme-linked immunosorbent plate. The optimum proportion of each ingredient in the kit is determined by experiments. The kit can be used for rapid diagnosis and detection of animal Peste des petits ruminants virus antibody, especially for the antibody detection of a lot of samples in the epidemiological survey of Peste des petits ruminants. The detection method of Peste des petits ruminants virus b-ELISA has different detection principle and experiment operating procedures and the like from those of a c-ELISA detection method in a BIRAD laboratory. The Peste des petits ruminants nucleoprotein antigen and Peste des petits ruminants monoclonal antibody in the kit are self-developed. The detection sensitivity, singularity and other indexes of the kit are the same with those of the c-ELISA detection method in the internationally recognized BIRAD laboratory.

Description

Technical field [0001] The invention involves the field of biotechnology, especially the field of virus antibody testing. Background technique [0002] The incidence and mortality of the pkrv (PPRV) of the Peste des Petits Ruminants Virus (PPRV) are as high as 100%and 90%of the mortality. In developing countries, they have caused serious socio -economic problems.Line as an important animal disease.The disease was first reported in Corteda, and most of the African countries that occurred south of Sahara and north of the equator, almost all countries from the Middle East to Turkey, were also widely spread in India, South Asia, and West Asia.In July 2007, the epidemic disease was first introduced to Tibet, my country. [0003] The method of detection method of small anti-spy epidemic virus antibodies, the recommended methods recommended by the World Animal Health Organization are virus neutralized test (VN) and competition Elisa (C-Elisa). Under general conditions, it can distinguis...

AI Technical Summary

Problems solved by technology

[0005] In the investigation of corresponding domestic diseases by using the c-ELISA diagnostic kit developed by the French BIRAD laboratory, we found that the purchase of this kit needs to be ordered from abroad in large quantities every time, without small packages, and the economic cost is relatively low. High, the substrate solution in the kit needs to be prepared in advance, and the operation is cumbersome

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