22results about How to "Improve overall performance" patented technology

The invention relates to a mouse anti-PBP2a hybridoma cell strain, a monoclonal antibody and application of the mouse anti-PBP2a hybridoma cell strain, staphylococcus aureus PBP2a protein is extracted as an antigen, after the high-purity antigen is obtained, a good immunoreaction is aroused in a mouse body, then a hybridoma technology is adopted, two cell strains with high affinity and specificity are screened, and the corresponding antibody is prepared; the two antibodies are used as a gold-labeled antibody and a coating antibody in a colloidal gold immunoassay kit, and the kit can be used for detecting PBP2a protein in methicillin-resistant staphylococcus aureus and can be used for developing related in-vitro diagnostic reagents.

The invention discloses a biomarker combination and application thereof in predicting embryo quality. Wherein the biomarker combination comprises small molecule non-coding RNA (Ribonucleic Acid) with nucleotide sequences as shown in SEQ ID NO.1-3; or micromolecule non-coding RNA as shown in SEQ ID NO.1-8; or micromolecular non-coding RNA as shown in SEQ ID NO.1-13. The invention also discloses a method for preparing the micromolecular non-coding The biomarker combination disclosed by the invention not only contains miRNA, but also contains multiple types of sncRNA such as tsRNA, snRNA, Y RNA, snoRNA and the like, and the biomarker combination has the characteristic of obviously distinguishing the embryo quality. When the biomarker combination is used for predicting the embryo quality, only an embryo culture solution is needed, and as a non-invasive sample collection operation, the shape and subsequent development of the embryo are not influenced. Meanwhile, the invention also provides a construction method and a prediction method of the embryo quality prediction model, and the final model has high sensitivity and high specificity, thereby providing guidance for embryo screening.

The invention discloses a tsRNA marker for diagnosing and evaluating myocardial aging, a kit and application of the tsRNA marker. The application of the tsRNA marker comprises one or more of the following applications: A1) application in preparation of a product for diagnosing myocardial aging; a2) in preparation of products for screening myocardial aging; a3) in preparation of a product for treating myocardial aging; a4) in preparation of myocardial aging condition assessment products; a5) in preparation of products for identifying and distinguishing myocardial aging and other heart diseases; the tsRNA marker is tsRNA-5006c-LysCTT, and the nucleotide sequence of the tsRNA marker is as shown in SEQ ID No. 1. The expression quantity of the tsRNA marker provided by the invention in the plasma of a myocardial aging patient is obviously increased compared with that of a healthy control person, which indicates that tsRNA-5006c-LysCTT has the potential to be used as the myocardial aging biomarker.

The utility model discloses a sealing ring with high toughness, which comprises a sealing ring main body, the sealing ring main body comprises a base layer, the outer side of the base layer is provided with a high-temperature-resistant layer, the outer side of the high-temperature-resistant layer is provided with a wear-resistant layer, and a plurality of uniformly distributed connecting blocks are embedded in the sealing ring main body. The sealing ring has good heat resistance and cold resistance, so that the sealing ring can be used in an extreme environment, the service life is prolonged, the wear-resistant layer is made of chloroprene rubber, the wear-resistant layer is located on the surface layer, the oil resistance is extremely good, the wear resistance is high, the heat resistance is good, the bonding force is high, the wear resistance of the sealing ring is effectively improved, and the service life of the sealing ring is prolonged. And the multiple connecting pieces are connected end to end through the connecting blocks to form a polygonal structure, the traction effect is achieved, the connecting pieces have the characteristic of high toughness, and the toughness and the deformation resistance of the sealing ring body can be enhanced.

The invention relates to the technical field of concealer cosmetics, and particularly discloses an emulsified concealer and a preparation method thereof. The emulsified concealer is prepared from the following raw materials in parts by weight: 1.5-4 parts of an emulsifier A; 0.5-1 part of an emulsifier B; 10 to 25 parts of grease; 5-9 parts of a forming agent; 0.1 to 2 parts of an antioxidant; 1-10 parts of a skin feeling modifier; 3-30 parts of a coloring agent; 10 to 50 parts of water; 0.5-2 parts of a stabilizer; 2 to 20 parts of a humectant; 0.5-1 part of a preservative; the preparation method comprises the following steps: mixing the emulsifier A, the emulsifier B, the grease and the forming agent, heating to 80-90 DEG C, and uniformly stirring; then adding a skin feeling modifier, and uniformly stirring and homogenizing at 80-90 DEG C; adding a coloring agent, and uniformly stirring and homogenizing to obtain a semi-finished product material; uniformly mixing the water, the stabilizer, the humectant and the preservative, adding the mixture into the semi-finished product, uniformly stirring and homogenizing at 80-90 DEG C, discharging and filling. The emulsified concealer provided by the invention not only is not easy to dull after being used, but also can give consideration to better waterproofness, so that a better application effect can be shown.

The application discloses an amphibious operation vehicle plate mud breaking device, and belongs to amphibious cleaning operation mechanical equipment. The device is composed of a connecting support, a driving unit and a rotary cutting roller, wherein the connecting support is used for connecting an amphibious operation vehicle body or a working arm; the driving unit comprises a mounting base, left and right side plates, a driving motor, a chain transmission mechanism and the like; a motor mounting plate is changed in position through an adjusting screw to tension the chain; a sealing plate is quickly wrapped around a spacer to form a sealed transmission space through buckles and hasp lock rings; the rotary cutting roller is a left-right symmetrical regular polygon hollow structure, equal-distance cutting blades with staggered bending directions at the ends are mounted on the rotary cutting roller, sealing rubber sheets are arranged at both ends of the rotary cutting roller, and an inner center pipe is hermetically welded with a side sealing plate to form a sealed cavity. The application can forcibly pre-cut and break the riverbed plate mud, so that the spiral mud pump can be smoothly sucked and transported, and has the characteristics of lightweight structure, convenient maintenance, no cutting blind area and small rotary resistance.

The invention discloses a method for determining the content of 2, 6-dipicolinic acid in bombyx batryticatus and a preparation thereof and application of the method, and belongs to the technical field of traditional Chinese medicine chemistry. According to the determination method, the content of the 2, 6-dipicolinic acid in the bombyx batryticatus and the preparation thereof is determined through a high performance liquid chromatography method, and the content of the 2, 6-dipicolinic acid serves as a characteristic peak in a liquid chromatography characteristic spectrum; therefore, the method is applied to quality detection of stiff silkworms and preparations thereof. The 2, 6-dipicolinic acid is used as an index component under quality detection items such as chemical identification and content determination of the stiff silkworm and the traditional Chinese medicine preparation containing the stiff silkworm, and the defect that the existing pharmacopoeia standard is lack of index components is overcome; the characteristic of the 2, 6-dipicolinic acid is obviously better than that of uracil, adenosine, guanosine and other nucleoside components, detection is facilitated, and a reference substance is cheap and easy to obtain and can be used as a characteristic peak for identification to improve the quality standard of the stiff silkworm formula granules.

The invention relates to a mechanical pressure element for a top weight arm (2) in a ring spinning machine. The mechanical pressure element comprises a spring unit (13) for providing a spring force for the top weight arm (2) and an adjusting device for adjusting the spring force. The mechanical pressure element is characterized in that it is designed to receive a discrete number of elements for selecting discrete spring force values of the spring unit (13).

The invention relates to the technical field of waterproof coiled materials, in particular to a weather-resistant fluorocarbon film waterproof coiled material and a preparation method thereof. According to the technical scheme, the waterproof coiled material comprises a waterproof coiled material body, the waterproof coiled material body comprises a surface layer, an anti-ultraviolet layer, a hydrophobic layer, a self-healing layer, a hydrophilic layer and a bottom layer from top to bottom, the surface layer, the anti-ultraviolet layer, the hydrophobic layer, the self-healing layer, the hydrophilic layer and the bottom layer are bonded with one another through hot-pressing compounding, and one side of the waterproof coiled material body is fixedly connected with a connecting piece; the surface of the connecting piece is provided with a composite surface, the composite surface is of a corrugated damping surface structure, one side of the waterproof roll body is fixedly connected with a clamping block, and a clamping groove is formed between the clamping block and the connecting piece; by accurately adjusting the mixing proportion of the fluorocarbon and the PVA, the proportion of the fluorocarbon can be increased in the environment needing higher waterproof performance, the content of the PVA can be increased in the construction occasion needing good adhesive force, and the adjusting capacity enables the surface layer to flexibly cope with different environments and requirements.

The present invention discloses a nanoprobe for detecting miRNA-21 and its preparation method and application, and relates to the field of bio-nanomaterials, including constructing a long RCA chain rich in G-quadruplex sequence by RCA reaction according to the sequence of miRNA-21, and loading a Blocker chain on the long RCA chain, so as to obtain a nanoprobe LGBN for detecting miRNA-21. The present invention proposes a nanoprobe of a long RCA chain loaded with a Blocker chain rich in a G-quadruplex sequence, the nanoprobe has a certain confinement effect, can significantly improve the reaction speed, and then the nanoprobe is prepared into a kit, which can be used for detecting serum samples, effectively reducing the reaction time, and having a lower detection limit. The kit proposed by the present invention can shoot the product through a portable mobile terminal, and the concentration of miRNA-21 can be obtained by reading and calculating in the cloud, which removes the limitation of the instrument, does not require complicated operation and large instruments, has the characteristics of simplicity, feasibility and portability, and thus builds a fast and simple POCT platform.

The invention discloses a method, reagent and application for identifying genus-level plants of the genus Paris, and relates to the technical field of plant identification, comprising the following steps: using the genomic DNA of the sample to be identified as a template, using primers Paris-F, Paris-R and probe Paris-probe to perform real-time fluorescence quantitative PCR amplification reaction, and detecting that the CT value is less than 40, then identifying it as a genus-level plant of the genus Paris; the sequence of the primer Paris-F is shown in SEQ ID No.1, the sequence of the primer Paris-R is shown in SEQ ID No.2, and the sequence of the probe Paris-probe is shown in SEQ ID No.3. The invention has excellent specificity and sensitivity, and can quickly and reliably identify genus-level plants of the genus Paris.

The utility model relates to the technical field of cables, and discloses a cable with low temperature resistance and frost crack prevention functions, which comprises four cable copper conductors, and the outer surfaces of the four cable copper conductors are coated with conductor insulating skins for protecting the cable copper conductors. When the cable is used, the heating wire starts to heat after being connected with a power supply, the outer surface of the constant-temperature layer is fixedly connected with the heat preservation layer, the heat preservation layer is made of a polyimide coating, polyimide has excellent anti-freezing and low-temperature-resistant performance, and the cold-resistant and anti-freezing performance of the cable can be further improved; the cable has the functions of heat insulation and heat preservation, low heat conductivity, stable heat insulation and heat preservation performance and excellent flame retardance, heat emitted by the heating wire can be well locked and is not prone to loss, and the stability of power transmission work is effectively guaranteed.

The utility model relates to the technical field of cables, and discloses a flexible cable with bending resistance, which comprises four cable copper conductors and a plurality of bending contraction strips, the outer surfaces of the four cable copper conductors are coated with conductor insulating skins for protecting the cable copper conductors, and the bending contraction strips are arranged on the cable copper conductors. The outer surfaces of the four conductor insulation skins are fixedly connected with a conductor fixing layer used for fixing four cable copper conductors, and four triangular elastic strips used for improving the strength of the cable are fixedly connected in the conductor fixing layer. When the cable is bent, the bending contraction strips located on the inner side of the bending extrude each other, so that the contraction gap and the bending seam area of the inner side of the bending can contract and become smaller, the stress is offset, the inner side of the cable cannot be compressed and cracked when the cable is bent, the bending stability of the cable is improved, and the service life of the cable is prevented from being shortened due to excessive bending of the cable.

The utility model belongs to the technical field of cables, and particularly relates to a hoisting B1-level fireproof cable for constructional engineering, which comprises a plurality of wire cores, an insulating layer is fixedly connected to the outer wall of each wire core, a first fireproof layer is fixedly connected to the outer wall of each insulating layer, a filling layer is fixedly connected to the outer wall of each first fireproof layer, and a second fireproof layer is fixedly connected to the outer wall of each filling layer. A plurality of mounting holes are formed in the filling layer, protective sleeves are fixedly connected to the inner walls of the mounting holes, load-bearing steel ropes are fixedly connected to the interiors of the protective sleeves, a shielding layer is fixedly connected to the outer wall of the filling layer, and a second fireproof layer is fixedly connected to the outer wall of the shielding layer. According to the utility model, through the design of the wire core, the effects that the compression coefficient of the conductor is effectively improved, the outer diameter of the conductor is reduced, the gaps among the wire cores are reduced, and the quality accident that the conductor is oxidized due to the fact that air and water enter the gaps of the conductor are reduced are achieved; through the design, the battery cell has good conductivity.

The invention relates to an apoA-I anti-sugar functional mutant as well as a preparation method and application thereof. The mutant is obtained by constructing an apoAE125C / E136C recombinant protein and crosslinking the recombinant protein into a dimer. The apoA-I anti-sugar functional mutant has superexcellent anti-sugar characteristic and biological safety, can remarkably improve the blood fat of diabetic mice, reduce ectopic fat deposition of the liver and inhibit formation of atheromatous plaques, and has a good market application prospect.

The invention discloses a making method of corn-wheat composite noodles added with arachin, and belongs to the field of food processing. The corn-wheat composite noodles are prepared from the following raw materials in percentage by mass: 70-76% of wheat flour, 21-25% of corn flour, 1-4% of arachin, 1-2% of table salt and 0.1-0.5% of dietary alkali. When the corn-wheat composite noodles are prepared, the overall quality of the corn noodles is improved in a mode of adding the arachin, the internal network structure of the noodles is more uniform, the quality is better, the requirements of zero food additives are met, and the addition amount of the corn flour is also increased.

The invention discloses an industrial non-intrusive measurement data transmission method and system based on wavelet transform compression. The method comprises the following steps: designing a data transmission protocol for I format information; the power system collects the equipment type, the operation mode, the real-time power, the real-time current, the transient power and the transient current on the production line, and uniquely encodes the equipment type and the operation mode; the power system monitors the start-stop state of electric equipment on the production line; transmitting a remote measurement value and a remote signaling value to a monitoring system; directly transmitting real-time power and real-time current through a network; the transient power and the transient current are compressed and then transmitted; the start-stop state of the electric equipment is directly transmitted through a network; data encryption is carried out before transmission; and the monitoring system carries out data decryption, verification and error processing on the received data. According to the invention, effective compression transmission of transient power and transient current data is realized, and high requirements of a modern industrial automation system on real-time performance and accuracy are met.

The invention discloses a method for making a coarse grain compound corn steamed bread, and belongs to the field of food processing. The invention comprises the following steps: S1, crushing the raw materials, passing through a 60-mesh sieve, and obtaining common corn flour, glutinous corn flour, and coarse grain flour; S2, compounding the raw material flour described in step S1 with wheat flour in different proportions, and the total corn flour content of the obtained compound flour A is 30% to 44%; S3, mixing the compound flour A described in step S2 with active dry yeast and double-effect baking powder, putting them into a dough mixer, adding water and kneading the dough, and obtaining a pre-proofing compound dough B; S4, proofing the pre-proofing compound dough B described in step S3, and obtaining a post-proofing compound dough C; S5, steaming the post-proofing compound dough C described in step S4 at 100° C. for 20 to 25 minutes, and obtaining a coarse grain compound corn steamed bread. The coarse grain compound corn steamed bread prepared by the present invention significantly increases the corn flour content in commercially available corn self-raising flour steamed bread by compounding common corn flour with glutinous corn flour, and the internal pore structure of the steamed bread is more uniform, and the quality of the corn steamed bread is better.

The invention relates to a primer, a probe and a detection method for rapidly detecting bone and joint infection pathogens. Comprising at least three groups of primers and probes in primers and probes respectively aiming at staphylococcus aureus, acinetobacter baumannii, staphylococcus epidermidis, staphylococcus hemolyticus, pseudomonas aeruginosa, enterobacter cloacae, escherichia coli, methicillin-resistant staphylococcus aureus and streptococcus pyogenes. According to the invention, the defects of small flux and few types of detectable pathogens in the existing bone and joint infection detection method are overcome, and 9 groups of specially designed primers and probes respectively aim at 9 bone and joint infection pathogens covering 90% or more of bone and joint infection cases; the kit has the advantages that the flux is large (at least three bone and joint infection pathogens can be detected at a time), multiple types of pathogens can be detected, and the specificity and the sensitivity are good.

The application relates to the field of biological medicine, and discloses a bifidobacterium adolescentis YF-01 and application of the bifidobacterium adolescentis YF-01 in fermented milk. The bifidobacterium adolescentis YF-01 is isolated from adult feces and is preserved in the China Center for Type Culture Collection, with a preservation number of CCTCC NO: M 20251308. Experiments prove that the bifidobacterium adolescentis YF-01 has good gastrointestinal fluid tolerance, the survival rate can reach 90.1% after being treated by simulated gastric juice for 3 hours, and the survival rate can reach 72% after being treated by simulated intestinal juice for 4 hours. The self-aggregation rate of the strain is 88.83%, and the hydrophobicity is 79.41%; the strain has significant scavenging capacity for DPPH free radicals and hydroxyl free radicals, and shows good antioxidant activity and adhesion capacity, which provides a theoretical basis for the application of the strain in fermented milk. The strain and metabolites thereof can be used for preparing food, medicine or health care products with the functions of regulating intestinal flora and enhancing antioxidant capacity, and have significant application potential in the development of functional fermented milk products.

The present application relates to a kind of protein markers for diagnosing postmenopausal osteoporosis and its application, belong to biological medicine technical field.The present application is screened, and it is found that NADH dehydrogenase 1 beta subcomplex subunit 4, NADH dehydrogenase 1 beta subcomplex subunit 5, NADH dehydrogenase 1 beta subcomplex subunit 6, NADH dehydrogenase 1, NADH dehydrogenase 1 alpha subcomplex subunit 8, cytochrome c oxidase subunit 7C, cytochrome c oxidase subunit 5A and cytochrome c oxidase subunit 7A2 can be used alone or in combination in the diagnosis test of postmenopausal osteoporosis, to accurately evaluate the PMOP state of subject.Further, the combination of NDUB5 and NDUA8 two protein markers shows excellent diagnostic sensitivity and specificity, which is helpful for early diagnosis of PMOP, screening of high-risk population of fracture and precise prevention and treatment.

This application discloses a compound, an electroluminescent device, a method for preparing the same, and a display device. The compound comprises Ar, a coumarin group, and a linking group B for connecting Ar and the coumarin group. Ar is selected from structural units containing aryl and / or heteroaryl groups. The method for preparing the electroluminescent device includes the step of preparing a hole transport layer. In the process of preparing the hole transport layer, a hole transport material layer is first prepared, the material of which includes the compound. Then, the hole transport material layer is treated with a first illumination condition to form a cross-linked structure. Next, an upper functional film is prepared on one side of the hole transport material layer. After the upper functional film is prepared, the hole transport material layer is treated with a second illumination condition to de-crosslink the cross-linked hole transport material.