Hepatitis A virus antibody colloidal gold detection kit

Abstract

The invention discloses an anti-HAV (hepatitis A virus) colloidal gold test kit. The anti-HAV colloidal gold test kit comprises a kit body, wherein a detection card is arranged inside the kit body; the detection card comprises a shell and a reagent strip packaged inside the shell; a sample adding hole and an observation hole are formed in the shell; the reagent strip comprises a bottom board; water absorption filter paper, a nitrocellulose membrane and a neutralization cushion coated with the anti-HAV, which are connected end to end are sequentially arranged on the bottom board; two detection lines coated with different detection anti-bodies are respectively arranged on the nitrocellulose membrane; a colloidal gold layer is arranged on the neutralization cushion; a protection cushion is arranged on the colloidal gold layer; the nitrocellulose membrane is correspondingly arranged at the position of the observation hole; the neutralization cushion is correspondingly arranged at the position of the sampling hole. The anti-HAV colloidal gold test kit provided by the invention has the advantages of strong specificity and high detection sensitivity, a hook effect can be effectively avoided without need of eluting a sample to be detected, and the situation that the false positive is removed and the weakly positive does not miss the inspection can be also ensured.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a hepatitis A virus antibody colloidal gold detection box for detection by a colloidal gold reagent nitrocellulose membrane adding method. Background technique [0002] Hepatitis A virus, referred to as hepatitis A, is an intestinal infectious disease caused by the hepatitis A virus (HAV). Hepatitis A is transmitted through fecal-oral transmission and is distributed worldwide, with different levels of control in different regions. It is still an important public health problem in developing countries, manifested as a high incidence and local epidemics, even outbreaks. The incidence of hepatitis A in my country accounts for about 50% of the total incidence of hepatitis. In 1988, when hepatitis A broke out in Shanghai, more than 310,000 people became ill. From April 25 to June 3, 2004, a vocational secondary school in Shaoshan City An outbreak of hepatitis A occurred, ...

AI Technical Summary

Problems solved by technology

With the development of colloidal gold technology, the hepatitis A virus IgM antibody detection reagent has the characteristics of fast, convenient, safe, and accurate results, but its hook effect (that is, HOOK effect, refers to false negatives caused by inappropriate antigen-antibody ratio) Phenomenon, in which the excess of antibody is called the prozone effect, and the excess of antigen is called the posterior zone effect) is very obvious. Some companies use the method of diluting the sample to be tested to avoid the hook effect, but because the sample to be tested is prone to IgG antibody interference substances, it is still not possible avoid false positives

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