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Integrated totally-closed detection reaction tube

A reaction tube and reaction technology, applied in bioreactor/fermenter combination, specific-purpose bioreactor/fermenter, laboratory container, etc., can solve problems such as rapid detection limit

Pending Publication Date: 2021-08-20
上海鲸舟基因科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although there are many existing nucleic acid detection technologies, PCR itself needs to rely on high-precision temperature cyclers for detection, and the pollution control is relatively strict. PCR-based RNA detection is limited to the central laboratory, which has great restrictions on rapid on-site detection. Faster, easier, cheaper and more accurate detection is still an important direction for improving detection technology

Method used

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  • Integrated totally-closed detection reaction tube
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  • Integrated totally-closed detection reaction tube

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0160] Embodiment 1 Contains the reaction tube of a reaction chamber and using method thereof

[0161] a. Upper tube

[0162] The sample cavity (101) has a volume of about 0.5ml and adopts a conical bottom surface to assist the liquid to guide to the bottom;

[0163] The reaction chamber (105), with a volume of about 300ul, is in a vacuum state before sample addition, and can be used to store freeze-dried reagents required for the reaction. The connection between the sample chamber and the reaction chamber is connected by a connecting pipe (103), the diameter of which is about 0.5mm, and sealed with paraffin;

[0164] The bottom of the upper tube and the bottom of the reaction chamber are heat-sealed with aluminum film;

[0165] The bottom outer wall of the upper tube is provided with threads.

[0166] b. down tube

[0167] The lower tube is a flat or round opaque plastic tube with a transparent observation window, which can accommodate a piece of colloidal gold lateral an...

Embodiment 2

[0171] Embodiment 2 contains the reaction tube of two reaction chambers

[0172] This embodiment is similar to Embodiment 1, the difference is that the upper tube contains 2 reaction chambers (105, 110), and the 2 reaction chambers are connected in series up and down, such as figure 2 shown.

Embodiment 3

[0173] Embodiment 3 The lower tube contains the reaction tube of 2 pieces of detection paper

[0174] This embodiment is similar to Embodiment 1, except that the lower tube contains 2 pieces of colloidal gold lateral analysis test paper, and the 2 pieces of test paper are placed face to face.

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Abstract

The invention provides an integrated totally-closed detection reaction tube. Specifically, the detection reaction tube comprises: an upper body, wherein the upper body is provided with a cover, a first cavity and a second cavity, the second cavity is located below the first cavity, the first cavity and the second cavity are provided with a fluid channel, and the fluid channel allows fluid to flow into the second cavity from the first cavity; and a lower body, and the lower body is provided with a detection cavity and a puncture component; The upper body and the lower body are hermetically connected together through a coupling structure. In addition, the invention further provides a corresponding detection method, a detection device and application. By means of the integrated totally-closed detection reaction tube, qualitative detection on bacteria and viruses containing target sequences and other nucleic acid-containing samples can be carried out quickly, conveniently and efficiently.

Description

technical field [0001] The invention relates to the technical field of nucleic acid detection, in particular to an integrated fully enclosed detection reaction tube. Background technique [0002] The method of specific detection of nucleic acid molecules (Nucleic acid detection) has important application value, such as the detection of pathogens (bacteria, viruses, and other nucleic acid-containing samples, etc.), the detection of genetic diseases, etc. In terms of pathogen detection, since each pathogenic microorganism has its unique characteristic nucleic acid molecular sequence, nucleic acid molecular detection for specific species can be developed, also known as nucleic acid diagnostics (NADs, nucleic acid diagnostics), in food safety, environment Microbial pollution detection, human pathogen infection and other fields are of great significance. Another aspect is the detection of single nucleotide polymorphisms (SNPs, single nucleotide polymorphisms) in humans or other ...

Claims

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Application Information

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IPC IPC(8): C12M1/00C12M1/24C12M1/34
CPCB01L7/52B01L3/50851B01L2400/0457B01L2300/0825B01L3/5023B01L2200/16B01L2300/0672B01L2300/087B01L2400/0683B01L2400/0406B01L2300/126B01L2300/044C12Q1/6844C12Q2531/101
Inventor 李明辉杨晓楠
Owner 上海鲸舟基因科技有限公司
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