Method for detecting metabolites of protopanaxadiol inside human body

Abstract

The invention relates to a method for detecting in-vivo metabolites of protopanaxadiol in blood plasma or urine samples, belonging to the field of pharmacoanalysis. According to the method, an ultra-high performance liquid chromatograph Agilent 1290 and a high-resolution mass spectrometer AB 5600+Q TOF are cooperatively used; an electrospray ionization (ESI) source is employed; a chromatographic column used in the invention is Agilent Eclipse plus C18RRHD with a filler particle size of 1.8 [mu]m, an inner diameter of 2.1 mm and a length of 50 mm; a mobile phase consisting of a 0.5% aqueous formic acid solution (A) and acetonitrile (B) is employed for gradient elution; flow velocity is 0.3 mL / min; column temperature is 30 DEG C; the elution procedures of gradient elution with the mobile phase comprise elution with 95% A in a time period of 0 to 1 min, elution with 95-75% A in a time period of 1 to 1.5 min, elution with 75-25% A in a time period of 1.5 to 6.0 min, elution with 25-0% A in a time period of 6.0 to 12.0 min, elution with 0% A in a time period of 12.0 to 13.0 min and elution with 0-95% A in a time period of 13.0 to 15.0 min; and qualitative detection is carried out the metabolites of protopanaxadiol inside the human body, and unknown metabolites are identified through comparison with blank samples. The method provided by the invention has the advantages of high speed, sensitivity, high resolution, high separation degree, etc.

Description

technical field [0001] The invention belongs to the field of pharmaceutical analysis, and relates to a liquid chromatography-mass spectrometry detection method, in particular to a detection method for metabolites of protopanaxadiol in a human body in a plasma or urine sample. Background technique [0002] The prior art discloses that protopanaxadiol is an in vitro hydrolyzed product of protopanaxadiol type ginsenoside, and its structure belongs to dammarane type tetracyclic triterpenoid ginsenoside compound, and its molecular formula is C 30 h 52 o 3 , with a molecular weight of 460.70 and a melting point of 197.5-198.5 degrees Celsius. Studies have shown that protopanaxadiol has pharmacological activities similar to ginsenosides, including anti-tumor effects, anti-epileptic seizures, and anti-depressant effects. At present, protopanaxadiol has entered the stage of clinical trials as a new antidepressant drug. Therefore, studying its metabolism in the human body plays an i...

AI Technical Summary

Problems solved by technology

[0003] Practice has shown that due to the low response of ginsenosides and the small dosage of human medicine, there are many endogenous substances with similar atomic masses in plasma, so it is difficult to detect them by conventional methods

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