Method and device for biological sample detection

Abstract

The invention provides a method and a device for the synchronous and rapid high-throughput and multiple-information detection of multiple indexes of various samples under a simple and improvised condition. The device adopted in the method belongs to a microflow control system, the width of a notch on the cross section of a groove is 0.01-5000 micrometers, and the vertical height from the notch tothe minimum point of the groove is 0.01-5000 micrometers, so that the entire detection device can perform information collection of various biological matters to the various biological liquid samplesor treated exsomatized solution in a 1 cm square space. The invention successfully takes the non-specific adsorption as the judging basis of the experimental result, saves the consumption of the reagent and the samples when rapidly finishing the experimental operation, retains the activity of the samples and the reagent, omits the steps of surface closing and cleaning in the prior detection method, reduces the complexity and the condition limitation of the experiment and both has the advantages of the immunoblotting and the enzyme-linked immunity.

Description

technical field [0001] The invention relates to a biological sample detection method and device, in particular to a method and device for quickly and easily performing high-throughput and high-information detection on biological samples. Background technique [0002] In modern scientific research, immunoblotting, enzyme-linked immunoassay (ELISA) and other methods are often used to study the interaction of substances. For example, in biological research, certain biomolecules are detected through the interaction between biomolecules. These two methods also have their own characteristics. For example, in HIV detection, immunoblotting requires multiple steps to detect multiple HIV antigens in the same serum, and then diagnose HIV. The ELISA is carried out in a multi-well plate, and there is an antigen interacting with the serum in each well, so this method can be used as a preliminary screening test. However, these two methods require professionals to operate, and the requirem...

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Problems solved by technology

At the same time, the amount of reagents required by these two methods is relatively large, including the consumption of antigens and buffers, and the amount of samples and related reagents used is mostly measured in hundreds of milliliters

[0003] In view of the shortcomings of the above two classical methods, the newly developed biosensor has more advantages as a supplement to these two methods, such as rapid detection, real-time observation, and high sensitivity, but it still needs to consume a large amount of Reagents and samples, the actual detection of samples also requires strict operation, and the preparation time of the experiment is very long (such as the surface treatment and the fixation of the biosensitive layer require a lot of time and strict condition requirements), and many times are required. In the cleaning step, the bigger problem is that the price of the required instruments is relatively high, and the requirements for the experimental conditions are also very high

In addition, for various biosensors that can perform real-time detection, it still takes a lot of time to modify the surface before adding the detection sample, and it needs to consume a certain amount of reagents to ensure the stability of the results obtained by the sensor. The probability of false positive is higher, so it is not suitable for practical detection and application

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