Method of detecting ochratoxin A in cereals by means of nano antibody and immunomagnetic separation

An immunomagnetic separation and ochratoxin technology, applied in the field of bacterial research, can solve the problems of high cost and long time consumption, and achieve the effects of low production cost, simple preparation and strong specificity

Inactive Publication Date: 2017-03-01
HAINAN UNIVERSITY
View PDF4 Cites 11 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a method for detecting ochratoxin A in grains based on nanobodies and immunomagnetic separation, aiming to solve the problems of high cost and long time-consuming of existing sample pretreatment methods

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method of detecting ochratoxin A in cereals by means of nano antibody and immunomagnetic separation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0043] In order to make the object, technical solution and advantages of the present invention more clear, the present invention will be further described in detail below in conjunction with the examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0044] The application principle of the present invention will be described in detail below in conjunction with the accompanying drawings.

[0045] Such as figure 1 As shown, the method for detecting ochratoxin A in grains based on nanobodies and immunomagnetic separation in the embodiment of the present invention comprises the following steps:

[0046] S101: Synthesize magnetic nanoparticles by chemical co-precipitation method, and couple the carboxyl group of the magnetic nanobeads with the amino group of the anti-OTA nanobody by EDC / NHS method to prepare IMB with high coupling ratio for enriching OTA in grain samples A...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a method of detecting ochratoxin A in cereals by means of a nano antibody and immunomagnetic separation. The method includes the steps of: 1) combining anti-OTA nano antibody with nano immunomagnetic beads to prepare IMB, and enriching the OTA in the cereals by means of the IMB to prepare a pretreated sample; 2) with dot immunization method, immobilizing an OTA-OVA detection antigen on a PVDF film, wherein the immunomagnetic beads are subjected to an immune-reaction in the form of antibody marker, the OTA-OVA and the to-be-tested substance, OTA, are combined with the antibody competitively so as to determine whether the immunomagnetic beads are aggregated on the PVDF film or not, thereby obtaining a detection result. The method achieves simple and quick qualitative detection. An operator can read the result just by naked eyes without any analysis instrument. Furthermore, the method can be used for developing a novel quick and visible detection card for specially detecting the OTA in the cereals.

Description

technical field [0001] The invention belongs to the technical field of bacteria research, and in particular relates to a method for detecting ochratoxin A in grains based on nanobody and immunomagnetic separation. Background technique [0002] Ochratoxin A (ochratoxinA, OTA) is a mycotoxin produced by certain species of Penicillin and Aspergillus, which mainly contaminates grains and their products, and is one of the main factors affecting food safety in my country. Therefore, it is particularly important to develop OTA rapid detection method. Mycotoxins are secondary metabolites produced by fungi and are commonly found in food and feed contaminated by fungi. Studies have shown that mycotoxins may cause liver, kidney and other organ lesions, such as hepatitis, liver cirrhosis and liver cancer, which is one of the main factors affecting food safety. Common mycotoxins in food include aflatoxins (AFs), ochratoxins (OTs), deoxynivalenol (DON), fumonisin B 1 (fumonisin b 1 ,F...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/543G01N33/68
CPCG01N33/54326G01N33/5434G01N33/6854
Inventor 刘星陈健曹献英孙志昶唐宗文马新辉舒杨
Owner HAINAN UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap