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Method of detecting ochratoxin A in cereals by means of nano antibody and immunomagnetic separation

An immunomagnetic separation and ochratoxin technology, applied in the field of bacterial research, can solve the problems of high cost and long time consumption, and achieve the effects of low production cost, simple preparation and strong specificity

Inactive Publication Date: 2017-03-01
HAINAN UNIVERSITY
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Problems solved by technology

[0004] The purpose of the present invention is to provide a method for detecting ochratoxin A in grains based on nanobodies and immunomagnetic separation, aiming to solve the problems of high cost and long time-consuming of existing sample pretreatment methods

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  • Method of detecting ochratoxin A in cereals by means of nano antibody and immunomagnetic separation

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[0043] In order to make the object, technical solution and advantages of the present invention more clear, the present invention will be further described in detail below in conjunction with the examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0044] The application principle of the present invention will be described in detail below in conjunction with the accompanying drawings.

[0045] Such as figure 1 As shown, the method for detecting ochratoxin A in grains based on nanobodies and immunomagnetic separation in the embodiment of the present invention comprises the following steps:

[0046] S101: Synthesize magnetic nanoparticles by chemical co-precipitation method, and couple the carboxyl group of the magnetic nanobeads with the amino group of the anti-OTA nanobody by EDC / NHS method to prepare IMB with high coupling ratio for enriching OTA in grain samples A...

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Abstract

The invention discloses a method of detecting ochratoxin A in cereals by means of a nano antibody and immunomagnetic separation. The method includes the steps of: 1) combining anti-OTA nano antibody with nano immunomagnetic beads to prepare IMB, and enriching the OTA in the cereals by means of the IMB to prepare a pretreated sample; 2) with dot immunization method, immobilizing an OTA-OVA detection antigen on a PVDF film, wherein the immunomagnetic beads are subjected to an immune-reaction in the form of antibody marker, the OTA-OVA and the to-be-tested substance, OTA, are combined with the antibody competitively so as to determine whether the immunomagnetic beads are aggregated on the PVDF film or not, thereby obtaining a detection result. The method achieves simple and quick qualitative detection. An operator can read the result just by naked eyes without any analysis instrument. Furthermore, the method can be used for developing a novel quick and visible detection card for specially detecting the OTA in the cereals.

Description

technical field [0001] The invention belongs to the technical field of bacteria research, and in particular relates to a method for detecting ochratoxin A in grains based on nanobody and immunomagnetic separation. Background technique [0002] Ochratoxin A (ochratoxinA, OTA) is a mycotoxin produced by certain species of Penicillin and Aspergillus, which mainly contaminates grains and their products, and is one of the main factors affecting food safety in my country. Therefore, it is particularly important to develop OTA rapid detection method. Mycotoxins are secondary metabolites produced by fungi and are commonly found in food and feed contaminated by fungi. Studies have shown that mycotoxins may cause liver, kidney and other organ lesions, such as hepatitis, liver cirrhosis and liver cancer, which is one of the main factors affecting food safety. Common mycotoxins in food include aflatoxins (AFs), ochratoxins (OTs), deoxynivalenol (DON), fumonisin B 1 (fumonisin b 1 ,F...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/543G01N33/68
CPCG01N33/54326G01N33/5434G01N33/6854
Inventor 刘星陈健曹献英孙志昶唐宗文马新辉舒杨
Owner HAINAN UNIVERSITY
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