35 results about "Leucomalachite green" patented technology

The invention provides haptens, immunogens comprising such haptens coupled to an antigenicity-conferring carrier material, conjugates comprising such haptens bonded to a labelling agent as well as, antibodies raised against such immunogens and capable of binding with leucomalachite green and malachite green.

The invention relates to a leucomalachite green gold-labeled test paper detection kit. The test paper detection kit has advantages of fast and accurate detection and high sensitivity, therefore, the invention also provides a preparation method of the detection kit. The detection kit includes a test strip which is packaged in a box shell body, and a sample injection hole and an observation hole are arranged on the box shell body, the test paper detection kit is characterized in that: the test strip takes polyvinyl chloride or polyethylene as a back lining, a sample injection area at one end ofthe test strip is affixed and adsorbed with a glass fiber film containing a conjugate of colloidal gold and anti-leucomalachite green monoclonal antibody; a cellulose nitrate film is affixed to a testing area in the middle of the test strip, a small section of the glass fiber film overlaps the cellulose nitrate film, and a water absorption area at the other end of the test strip is affixed with apiece of absorbent paper; a detection line with material of recessive malachite green-egg white albumin and a quality control line with material of goat anti-mouse IgG are arranged in the direction from the sample injection area to the water absorption area sequentially, and the cellulose nitrate film is covered with a bovine serum albumin layer with the concentration of 1 percent; and the sampleinjection hole is corresponding to the glass fiber film in the sample injection area of the test strip, and the observation hole is corresponding to the testing area of the test strip.

The invention discloses an immune colloidal gold test stripe for detecting leucomalachite green in aquatic products and a preparation method; the colloidal gold test stripe provided by the invention detects leucomalachite green with a direct competition method, and comprises a sample pad, a colloidal gold combined pad, a nitrocellulose membrane (NC), a water adsorption pad and a PVC back lining, one end of the PVC back lining is sequentially adhered with the sample pad and the colloidal gold combined pad, and the middle thereof is adhered with the NC layer, and the other end thereof is sequentially adhered with water adsorption pad; the immune colloidal gold test stripe has the characteristics that colloidal gold combined pad is enveloped with colloidal gold marker which is monoclonal antibody of the specificity of the leucomalachite green, and the NC is adsorbed with leucomalachite green-OVA and sheep anti-mouse IgG. The invention has high detection sensitivity and low price and is simple and rapid, the whole reaction only needs 30 minutes; the immune colloidal gold test stripe can be used for large-batch screening of samples and can rapidly detect the residual leucomalachite green in the aquatic products at site.

The invention discloses a toxicant elimination and fish-odor removal culturing method for soft-shelled turtles. According to the method, rotational flows are generated with oxygenic small molecular group water in a culture pond capable of generating the rotational flows in an intermittent mode, so that the culturing mode that the turtles swim for 30 minutes and rest for 90 minutes is achieved. All residual medicines, including leucomalachite green, of the cultured turtles are not detected, and therefore the turtles become delicious foods or high-quality health care products.

The invention provides a pre-column electrochemical derivatization one-time total quantity measuring method for malachite green and leucomalachite green in aquatic products and belongs to one-time total quantity measuring methods for converting leucomalachite green into malachite green. The key of the method is that it is found that after a leucomalachite green solution is subjected to oxidation voltage +650 mv of an electrochemical Coulomb detector (ECD), averagely 98.86% of leucomalachite green is converted into malachite green, original malachite green is unchanged, and the total concentration of leucomalachite green and malachite green can be measured through a high performance liquid chromatograph in the one-time measurement process of leucomalachite green and malachite green in a sample. Sample pre-treatment time is greatly shortened, use of reagent consumables is reduced, and electrochemical precolumn derivatization is used for replacing post-column derivatization of lead dioxide in the national standard. The method has the advantages of being simple in sample pre-treatment, good in purification effect, high in derivatization rate and high in recovery rate.

The invention provides an immunodetection method capable of detecting Furaltadone metabolite AMOZ, MG (malachite green) and LMG (leucomalachite green) simultaneously. An AMOZ hapten and an LMG hapten are synthesized scientifically and coupled with carrier proteins respectively, and artificial antigens are prepared. Hybridoma cell strains aiming at the two objects are prepared respectively from the AMOZ artificial antigen and the LMG artificial antigen, a hybrid-hybridoma cell strain capable of secreting an anti-AMOZ and anti-LMG bispecific antibody is prepared with a hybrid-hybridoma technique, a multi-residue immunodetection method is established successfully, simultaneous detection of AMOZ, MG and LMG is realized, the limit of detection of AMOZ is 0.1 ng / mL, and the IC50 is 1.3 ng / mL; the limit of detection of LMG is 4.8 ng / mL, and the IC50 is 45.3 ng / mL. The method has high specificity and higher sensitivity, has broad application prospect in multi-residue detection of AMOZ, MG and LMG in food and is simple and convenient to operate.

The invention relates to a method for determining malachite green, quinolone and sulfonamide drugs in aquatic products. The method comprises the following steps: step 1, preparing samples; step 2, extracting to-be-detected samples to obtain to-be-processed samples; step 3, determining chromatographic conditions; step 4, determining mass spectrum conditions; step 5, drawing a standard working curve; step 6, determining sensitivity, accuracy and precision. With adoption of the method, malachite green, leucomalachite green, quinolone and sulfonamide drugs in an extracted and concentrated aquatic product substrate are determined simultaneously for the first time, the problems that time is consumed and detection cost is high due to traditional respective extraction are solved, ion response value is increased, sensitivity is enhanced, detection limit is low, the effects of being qualitative, quantitative, accurate, fast, efficient and sensitive are realized truly, and the method can be taken as a reliable detection means for the three drugs and is applicable to extraction and concentration of a batch of samples.

The invention discloses malachite green hapten, malachite green artificial antigen and preparation methods of the malachite green hapten and artificial antigen. The malachite green hapten retains the feature structure of malachite green, and has active arms which are derived from different microdomains of the structure of malachite green, have different electron cloud densities and different carbon chain lengths, are suitable to be coupled with macromolecule carriers, and are amide linkage with amino or carboxyl tail end. The malachite green artificial antigen prepared with leucomalachite green hapten has very high specificity and sensitivity when applied to detection of the residual quantity of leucomalachite green and malachite green, and is high in accuracy and recovery. Moreover, the methods are simple, the cost is low, and a fast, sensitive, economical and efficient malachite green immunodetection method is established.

The invention discloses a method for degrading the malachite green waste water. The malachite green is degraded through oxidation after reduction, the malachite green can be reduced into the leucomalachite green to be precipitated in a short five hours, and accordingly a COD (Chemical Oxygen Demand) value of the waste water is reduced, meanwhile the chemical raw material leucomalachite green is obtained, malachite green large molecules are decomposed into small molecules of pollutants, and the further degradation of the malachite green is facilitated and the malachite green waste water which is not fully degraded is oxidized by an oxidation method and accordingly the degradation time is reduced and the energy consumption of the electrolysis is reduced.