Concealed malachite green gold mark detection test paper box and method for making same

Abstract

The invention relates to a leucomalachite green gold-labeled test paper detection kit. The test paper detection kit has advantages of fast and accurate detection and high sensitivity, therefore, the invention also provides a preparation method of the detection kit. The detection kit includes a test strip which is packaged in a box shell body, and a sample injection hole and an observation hole are arranged on the box shell body, the test paper detection kit is characterized in that: the test strip takes polyvinyl chloride or polyethylene as a back lining, a sample injection area at one end of the test strip is affixed and adsorbed with a glass fiber film containing a conjugate of colloidal gold and anti-leucomalachite green monoclonal antibody; a cellulose nitrate film is affixed to a testing area in the middle of the test strip, a small section of the glass fiber film overlaps the cellulose nitrate film, and a water absorption area at the other end of the test strip is affixed with a piece of absorbent paper; a detection line with material of recessive malachite green-egg white albumin and a quality control line with material of goat anti-mouse IgG are arranged in the direction from the sample injection area to the water absorption area sequentially, and the cellulose nitrate film is covered with a bovine serum albumin layer with the concentration of 1 percent; and the sample injection hole is corresponding to the glass fiber film in the sample injection area of the test strip, and the observation hole is corresponding to the testing area of the test strip.

Description

(1) Technical field [0001] The invention relates to a device for rapid diagnosis of drugs in aquatic meat food and a preparation method thereof, belonging to the field of biotechnology, in particular to a hidden malachite green gold label detection test paper box and a preparation method thereof. (2) Background technology [0002] Leucomalachite green (LMG) is the main metabolic residue of malachite green (MG) in organisms, which has a carcinogenic risk to humans. [0003] Malachite green, also known as aniline green, Victoria green or Chinese green, is a green crystal with metallic luster, soluble in water, ethanol and methanol, and the color of the aqueous solution is blue-green (the maximum absorption wavelength is 618nm). It was first introduced in 1913 In 2000, it was discovered that dyes such as malachite green can destroy pathogenic bacteria without causing damage to the host, so that these dyes can be used as antiseptics, trypanocidal drugs and other medical effects....

AI Technical Summary

Problems solved by technology

Chromatography (HPLC) detection technology is mainly used to detect LMG at home and abroad. Although it is sensitive and quantitatively accurate, it is slow in large-scale detection and relatively high in cost.

The rapid screening method represented by the gold standard detection test kit is the mainstream technology of immunology. It is fast, low-cost, and can be tested in large quantities. There is no report on this technology at home and abroad.

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