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Quick extraction and detection method of Leucomalachite green in freshwater fish

A technology of recessive malachite green and detection method, which is applied in the directions of measuring devices, instruments, scientific instruments, etc., can solve the problems of high detection cost and poor sensitivity of enzyme-linked immunosorbent assay, and achieves easy popularization and utilization, low cost, and extraction rate. excellent effect

Inactive Publication Date: 2019-09-06
GUANGXI UNIVERSITY OF TECHNOLOGY +1
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Problems solved by technology

There are Raman spectroscopy and enzyme-linked immunoassay for rapid detection of MG and LMG, but the sensitivity of Raman spectroscopy is poor, the detection cost of enzyme-linked immunoassay is high, and there are false negatives and false positives

Method used

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  • Quick extraction and detection method of Leucomalachite green in freshwater fish
  • Quick extraction and detection method of Leucomalachite green in freshwater fish
  • Quick extraction and detection method of Leucomalachite green in freshwater fish

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Embodiment Construction

[0049] Below in conjunction with specific embodiment, further illustrate the present invention. It should be understood that these examples are only used to illustrate the present invention and are not intended to limit the scope of the present invention. In addition, it should be understood that after reading the content taught by the present invention, those skilled in the art can make various modifications or changes to the present invention, and these equivalent forms also fall within the scope defined by the appended claims of the present application.

[0050] An algorithm code and the calculation process description of the present invention

[0051] MATLAB software calculation code, the calculation process of the present invention is done as follows demonstration:

[0052] A=sumabsorb('grass carp'); (convert the spectral signal of the "grass carp" sample into absorbance values ​​at different times and assign them to A)

[0053] B=A(:,[1:3000 3400:6000]); (deduct the co...

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Abstract

The invention relates to a detection method of Leucomalachite green, and specifically relates to a quick extraction and detection method of Leucomalachite green in freshwater fish. The method comprises the following steps: firstly, quickly extracting malachite green and Leucomalachite green in the freshwater fish, adding potassium borohydride powder to convert the malachite green into the Leucomalachite green, collecting the multi-wavelength chromatographic data through chromatography-spectrum combination, and treating to obtain a background spectrum database N not containing the Leucomalachite green; secondly, establishing a standard spectrum database V of the Leucomalachite green; thirdly, quickly extracting the freshwater fish sample on the market, adding the potassium borohydride powder, and then collecting a sample ultraviolet-visible spectrum c; and fourthly, quickly computing the total amount of the Leucomalachite green in the to-be-detected sample by adopting a vector-sub-spaceincluded criterion algorithm. The problem that the existing method for extracting the malachite green and the Leucomalachite green is tedious, complex in operation and low in extraction rate is solved, and the quick detection of the Leucomalachite green in the freshwater fish is realized.

Description

technical field [0001] The present invention relates to the detection method of recessive malachite green, be specifically related to the rapid extraction and detection method of recessive malachite green in freshwater fish. Background technique [0002] Malachite green (MG for short) is a triphenylmethane-type dye, which is a toxic triphenylmethane chemical substance. It is often used to prevent and treat saprolegniasis, gill mold, and cucumber. Insect diseases, etc. and fish preservation. [0003] After MG enters the human or animal body, it can produce Leucomalachite Green (LMG for short) through biotransformation. LMG is sensitive to light and has the strongest absorption peak at 260nm, which is carcinogenic, teratogenic and mutagenic and other side effects. [0004] my country's "NY5071-2002 Guidelines for the Use of Pollution-Free Food and Fish Medicine" has listed MG as a banned drug. MG will be converted into LMG in the fish body and stay for a long time, because ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06G01N30/02
CPCG01N30/02G01N30/06G01N2030/047G01N2030/067
Inventor 刘柳韦媛媛徐传杰忻欣林华韩莹粟晖董华秀
Owner GUANGXI UNIVERSITY OF TECHNOLOGY
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