8results about How to "Improve extraction yield" patented technology

The invention provides a preparation method of a general flavone extract in abelmoschus manihot flowers, which comprises the following steps: A) crushing the abelmoschus manihot flowers, extracting with an alcohol solvent, filtering, and concentrating to obtain an extracting solution; b) purifying the extracting solution by adopting macroporous resin to obtain an eluent; and C) concentrating the eluent, and drying to obtain the traditional Chinese medicine composition. According to the method, diluted alcohol extraction and a high-speed homogenization-ultrasonic-assisted liquid phase extraction method (HSH-UAE) are adopted, so that the contact area of a solvent and the abelmoschus manihot flowers is increased, full contact of medicinal materials and the solvent is guaranteed, and flavonoid substances are dissolved more sufficiently, so that the extraction speed is increased, and the extraction efficiency is improved. Macroporous adsorption resin is selected for separation and purification, the purification effect is good, operation is easy, safety, no toxicity and no pollution are achieved, reuse can be achieved after slight treatment, and the production cost is effectively reduced. Compared with a traditional method, the method has the technical advantages that the extraction speed is high, the extraction yield is high, effective components are fully extracted and reserved, and the process is simple.

The invention discloses a blood genome DNA extraction kit and an extraction method, and belongs to the technical field of nucleic acid extraction. The DNA extraction kit comprises a lysis solution, a first rinsing solution, a second rinsing solution and an eluent, wherein the lysate is prepared from the following components: 5.5 to 6 M of guanidine hydrochloride, 0.2 to 0.3 M of NaCl, 0.01 to 0.015 M of EDTA (Ethylene Diamine Tetraacetic Acid), 0.01 to 0.015 M of glucan, Triton X-100 with the volume concentration of 1.5 to 2 percent, 0.06 to 0.1 M of dithiothreitol, Tween-20 with the volume concentration of 5 to 10 percent and 10 to 50 mM of trehalose. The components of the lysate in the kit are optimized, through the synergistic effect of the components, the cell lysis efficiency is remarkably improved, meanwhile, DNA molecules higher in purity, more complete in structure and higher in extraction yield can be obtained, and therefore the kit has good application prospects.

The present application provides a composite extraction tower and its application. The tower body of the composite extraction tower of the present application is composed of a tower top, an extraction section and a tower bottom from top to bottom; a heavy phase liquid inlet pipe and a light phase liquid outlet pipe are respectively provided at the tower top, and a heavy phase liquid outlet pipe and a light phase liquid inlet pipe are respectively provided at the tower bottom; at least one group of mass transfer components are sequentially provided in the extraction section from top to bottom, and each group of mass transfer components sequentially includes a liquid gathering area and a liquid separation area from top to bottom, and a plurality of baffles are provided in the liquid gathering area, and all the baffles are sequentially distributed along the height direction of the tower body, and the liquid separation area is filled with packing rings for liquid separation. The composite extraction tower of the present application has a high separation efficiency and can improve the extraction yield.

The invention relates to the technical field of natural polysaccharide extraction, and discloses a method for efficiently preparing fucoidin by taking kelp as a raw material, which comprises the following steps: S1, selecting fresh kelp and cleaning; s2, cutting and drying the cleaned kelp; s3, adopting freezing-unfreezing treatment to destroy the cell structure; s4, adding a nano material to enhance the extraction efficiency; s5, performing ultrasonic-assisted extraction by using deionized water; s6, obtaining fucoidin through the steps of centrifugal separation and membrane separation; and S7, performing freeze concentration and vacuum freeze drying to obtain a final product. Freezing-unfreezing treatment and ultrasonic-assisted extraction are combined, the extraction efficiency of fucoidin is remarkably improved, the defects of a traditional extraction method are overcome, meanwhile, food-grade modified nano materials are adopted for enhancing polysaccharide release, different components are effectively obtained through a multi-round extraction strategy, and higher extraction yield and diversified application potential are provided.

The present invention relates to a process for the production of 5-hydroxymethylfurfural (5-HMF) comprising, in order: a) contacting a sugar feedstock with a polar aprotic synthesis solvent and an acidic dehydration catalyst at a temperature of 30-200 DEG C and a pressure of 0.001-10 MPa to produce a synthesis effluent comprising 5-HMF; b) concentrating the 5-HMF in the synthetic effluent; c) contacting the concentrated synthetic effluent with an aqueous stream; d) liquid-liquid extraction with an organic extraction solvent, followed by step e) backwashing with an aqueous solvent to obtain an organic raffinate rich in 5-HMF and solvent. The optionally concentrated organic raffinate is then subjected to a purification step g), for example water distillation or crystallization, to obtain a 5-HMF stream, for example in the form of an aqueous 5-HMF solution or crystalline 5-HMF.

This utility model relates to pectin extraction using ohmic heating technology and automated control technology, belonging to the field of food processing and automation control. Specifically, it is a pectin extraction device based on ohmic heating, which includes a reaction device, a storage and collection device, a pipeline conveying device, auxiliary devices such as pressure gauges and coolers, as well as software programs and hardware circuits. This utility model has a compact structure and simple operation. Through the rational design of the reaction vessel, ohmic heating technology can be applied and the stirring requirements of the processing can be met, realizing the automation of the pectin extraction process. It can precisely control the heating rate and temperature during the pectin extraction process, and can uniformly heat the material in a shorter time, achieving low energy consumption, high efficiency, intelligence, and green energy saving.

The invention belongs to the technical field of plant fermentation equipment, and particularly relates to ultrasonic plant fermentation extraction separation equipment which comprises an ultrasonic equipment body, a fermentation chamber is arranged in the ultrasonic equipment body, a fermentation stirring module and a centrifugal vibration unit are arranged in the fermentation chamber, and the fermentation stirring module comprises a fermentation box. A hollow cylinder is arranged in the fermentation box, a stirring shaft is axially and rotationally distributed at the circle center of the hollow cylinder, a stepping motor is mounted at the top of the stirring shaft, the stirring shaft is connected with a driving gear, and centrifugal vibration units are arranged on two sides of the driving gear. Energy storage is completed through an energy storage spring under the condition of centrifugal rotation, stirred plants and an ethanol water solution are rapidly oscillated through large diffusion vibration of a vibration expansion plate in a special shape, decomposition of plant cell walls and the oscillation degree in a hollow cylinder are further accelerated in cooperation with an ultrasonic equipment body, and the plant cell walls are rapidly decomposed. Mechanical vibration and stirring are linked, and needed assistance is provided for decomposition and fermentation of plant cell walls at the same time.

The invention discloses a preparation method of a tobacco flavor capable of reducing harmful substance residues, which comprises the following steps: adding an aqueous solution or an ethanol solution with the mass concentration of 10-80% into a tobacco raw material, carrying out hot reflux extraction at 30-100 DEG C, and filtering to obtain a tobacco extracting solution. And carrying out vacuum concentration on the tobacco extracting solution at 30-100 DEG C until the density is 1.0-1.1 g / cm < 3 >, so as to obtain the tobacco concentrated solution. Standing the tobacco concentrated solution, and filtering to obtain an external water phase solution; the preparation method comprises the following steps: mixing a surfactant, a carrier and an oil phase, adding an internal water phase solution, and stirring in an emulsification container to prepare a stable W / O / W type emulsion; uniformly mixing an external water phase solution with the W / O / W emulsion, stirring, standing for 10-60 minutes, layering, separating an emulsion phase from a water phase, collecting the water phase, adding propylene glycol with the mass ratio of 10%-20%, and performing vacuum concentration at the temperature of 30-70 DEG C until the density is 1.1-1.3 g / cm < 3 >, so as to obtain the tobacco flavor capable of reducing harmful substance residues. The tobacco flavor obtained by the method disclosed by the invention can effectively reduce the content of harmful substances such as heavy metals, and is very suitable for industrial production.