147results about How to "Improve extraction yield" patented technology

The invention relates to the essential oil of tobaccos as well as a preparation method and application thereof to cigarettes. The essential oil of the tobaccos is prepared by the following method of: taking discarded / defective tobacco wastes, extracting by an organic solvent, taking the crude extract of the essential oil, introducing water vapor, condensing and separating oil and water so as to obtain the essential oil of the tobaccos. By adding the essential oil of the tobaccos, which is disclosed by the invention and is used as a flavoring agent, into the tobacco shreds of cigarettes, the self fragrance of the tobaccos can be increased, the quality of the fragrance of the cigarettes is enhanced, the excitation and the miscellaneous gas of the tobaccos are reduced, and smoke is softened. The essential oil of the tobaccos as well as the preparation method and the application thereof to the cigarettes have the advantages that the advantage of higher extracting yield of a solvent extracting method and the characteristic of refinement of a water-vapor distillation extracting method are combined, the yield of the essential oil of the tobaccos is obviously higher than that of a traditional water-vapor distillation method, and extracting equipment and a solvent are simple and convenient and are easy to obtain; and moreover, no solvent residue exists in a final product of the essential oil of the tobaccos, and the safety of the product is high.

The invention provides a preparation method of high-purity collagen protein sponge, and relates to a preparation method of collagen protein sponge. The preparation method of the high-purity collagen protein sponge is used for solving the problems that the collagen protein sponge prepared by using a conventional method is long in production cycle and low in yield and purity and has poor hemostasis performance. The preparation method of the high-purity collagen protein sponge comprises the following steps: step one. pretreating fresh bovine heel tendons; step two. extracting collagen protein; step three. centrifuging; step four. salting out; step five. dissolving; step six. carrying out gradient dialysis; step seven. pre-freezing; step eight. carrying out freeze drying; and step nine. sterilizing. The final product prepared by using the method has a smooth and flat surface and relatively good hemostatic performance and is uniform in pore size distribution. The product has relatively high purity (the total amount of amino acids reaches 97.73%), an obvious hemostatic effect and no abnormal taste, is safe, non-toxic, high in yield and short in time; liquid is clear without impurities; the production cycle is shortened; the whole preparation process is carried out at a room temperature; the biological activity of the collagen protein is maintained; and the application of the high-purity collagen protein sponge in clinical is improved.

A kind of bead check separation setting, it includes the first reaction groove, the second reaction groove, the third reaction groove, the first miniflow pipe and the second miniflow pope. The first reaction groove has mixture liquid, the mixture liquid includes multiply bead and check extraction, the check extraction combines with the bead. The second reaction groove has cleanout buffer solution to clean the bead. The third reaction groove has separation buffer solution to separate the bead and the cleanout buffer solution. The first miniflow pope is used to connect the first reaction groove and the second reaction groove, and allows the bead to move from the first reaction groove to the second reaction groove. The second miniflow pipe is used to connect the second reaction groove and the third reaction groove, and allows the bead to move from the second reaction groove to the third reaction groove.

The invention provides a method for extracting propolis by simulative biological fluid bed supercritical CO2 fluid, which comprises that: bran (or rice bran and the like) taken as biomembrane carrier and original gum particles after physical treatment simulate a fluid bed in an extraction kettle to perform gridded filling, the supercritical CO2 fluid is used for extraction, and the lowest speed of a granular bed converting from a stationary state to fluidization is regulated by regulating temperature, pressure and flow rate of the fluid in the extraction process so as to reach the critical fluidization state; therefore, high-quality and high-yield propolis extract is obtained through a cyclic process of fluidization diffusion, dissolution and separation between granules and the fluid. The method improves the yield of the propolis extract, reduces the cost, and extracts nearly all terpene compounds and 40 to 50 percent of flavonoid compounds in the propolis at the same time; and products do not have organic solvent.

The invention discloses a method for preparing tea saponin by using tea seed degreased dregs, which comprises the following process steps of: firstly extracting by using high-concentration aqueous methanol, recovering methanol from an extraction liquid to prepare coarse tea saponin pasty liquid, and then purifying by adopting a three-purification method. Chitosan is used as a flocculating agent in the first purification to filter and remove partial impurities; a calcium hydroxide emulsion is used as a precipitating agent in the second purification, so that the tea saponin is converted into tea saponin calcium to be precipitated and separated from the water-soluble impurities, then the tea saponin calcium is converted into the tea saponin to be dissolved in water by using ammonium hydrogencarbonate, the impurities are further removed through filtration, then 30% hydrogen peroxide is used for decoloring, and the finished product of the tea saponin is prepared finally through spray drying. A solid obtained after separating and recovering the methanol from a methanol-contained solid obtained after extraction can be used as livestock feed. The invention has the advantages that the method has high obtainment ratio of the tea saponin, low energy consumption, simple process, convenient operation, high quality of the produced finished product of the tea saponin and wide application range.

The invention discloses a method for extracting tea saponin from oil-tea-cake. The method comprises the following steps of: leaching, namely performing continuous countercurrent leaching or multiple intermittent leaching on oil-tea-cake, of which residual oil is extracted and which is dried, by using C1-C6 alcohol as a solvent; concentrating, namely concentrating extracting solution until the solution is supersaturated, wherein the concentration operation is performed under micro-negative pressure; cooling and filtering, namely cooling concentrated solution and filtering, wherein the cooling temperature is room temperature to 0 DEG C; and drying, namely drying and desolventizing a solid obtained after cooling and filtration, wherein the dried and desolventized solid is a finished product of tea saponin powder. The method has the advantages that: (1) the extraction yield of the tea saponin is high, and the residual amount of the tea saponin of the oil-tea-cake in which the tea saponin is extracted is less than 2 percent; (2) the extraction and concentration operation is performed under micro-negative pressure, and the problem that safety accidents are caused by the volatilization of a large quantity of organic solvents in leaking workshops is solved; (3) the purified tea saponin has high purity and good color and luster; and (4) the process is simple, production cost is low, energy consumption is low, the yield is over 90 percent, the purity of an obtained tea saponin product is more than or equal to 91 percent, and the method is suitable for industrialized production.

The invention belongs to the field of molecular biology and particularly relates to a kit for extracting ribonucleic acid (RNA) by virtue of a paramagnetic particle method and an extraction method. The kit contains tissue digestion fluid, lysate, proteinase K, DNase I, DNase IBuffer, nucleic acid, extraction magnetic beads, washing liquid I, washing liquid II and eluant. The invention further discloses a method for extracting the tissue RNA by virtue of the kit. According to the kit and the method, the extraction yield and purity of RNA are increased, the integrity of RNA is improved, meanwhile, the automatic extraction is realized, and the simultaneous parallel testing of multiple samples is realized, so that the labor cost and the time cost are saved.

The invention discloses a seedless grosvenor momordica with high mogroside content, high whole fruit utilization rate and good taste, and a cultivating method for the seedless grosvenor momordica. The steps of the method are as follows: 1) cultivating and planting the diploid female and male seedlings of the grosvenor momordica; 2) when a male plant emerges a flower bud, inducing the formation of diploid big pollen; 3) picking the male flower that is induced to bloom and screen a diploid big pollen therein; 4) hybridizing the haploid oocyte of the diploid female plant after treating the diploid big pollen in the pollen germination solution, acquire the hybrid seeds after the female plant fruits; 5) propagating the hybrid seed into whole plant and then screening a triploid plant with the Chromosome number inspection; 6) planting the triploid plant and the diploid male plant, and carrying out artificial pollination to the male triploid plant with the male diploid plant when the plant is blooming, thus acquiring the seedless grosvenor momordica when the female plant fruits.

The invention relates to a kit for extracting blood genome DNA based on a magnetic bead method. The kit comprises a cell lysis solution, a proteinase K solution, a magnetic bead dispersion solution, a first cleaning solution, a second cleaning solution and an elution solution, wherein the cell lysis solution comprises a guanidine compound, sodium chloride and a tween 20; a magnetic bead in the magnetic bead dispersion solution has the characteristics that a core is superparamagnetic ferroferric oxide, the surface is coated with silicon hydroxyl, and the particle size is 200-800nm; the first cleaning solution comprises Tris alkali, EDTA, isopropanol and water; the second cleaning solution is an ethanol solution; and the elution solution is Tris alkali or de-ionized water. The kit is simple to operate and can greatly improve working efficiency of blood genome DNA extraction; without pretreating erythrocytes, the cell lysis solution is directly adopted for pyrolyzing blood cells, and the magnetic bead dispersion solution can be added and combined with the genome DNA; and the kit is high in extraction volume and suitable for high-throughput experiments.

A Se-enriched protein and a Se-enriched amino acid are prepared from Se-enriched fresh silkworm chrysalis through drying, deoiling, extracting protein in diluted alkali solution, regulating pH value to educe Se-enriched protein, centrifugal treating, depositing, freeze drying to obtain powder, and hydrolyzing to obtain Se-enriched amino acid. They can be used to prepare food for preventing cancer.

The invention relates to a soil humus extraction method and concretely relates to a soil humus extraction and purification method. The method comprises carrying out full concussion and dissolution on a sample to be extracted orderly through hydrochloric acid and a sodium hydroxide solution so that dissolution of organic matters to be extracted in an acid solution and alkali lye is promoted and carrying out separation and purification to obtain different humus substances of the sample to be extracted. The method improves a standard method provided by the international humic substances society, has simple processes and has good applicability. The extract obtained by the soil humus extraction method satisfies international humic substances society requirements. The method realizes maximized separation of products from soil without influence on a substance structure.

The invention provides a process for cleanly preparing cellulose by utilizing lignocellulose, belonging to the field of the biochemical industry. The method comprises the following steps of: using agricultural wood fiber wastes as raw materials, carrying out acid and steam explosion pretreatment and removing hemicellulose; adding steam exploded materials, organic solvents, alkalis and water to a high pressure reactor, raising the temperature to 80-280 DEG C, reacting at the temperature for 1-5 hours, reducing the temperature after reaction ends and carrying out filtration, thus obtaining weakly alkaline organic solvent materials, wherein the concentrations of the organic solvents are 40-90%; the additive amount of the alkalis accounts for 0.01-0.2% of the amount of the exploded materials; and treating the weakly alkaline organic solvent materials by catalyzing carbonic ester by hydrogen peroxide to generate peroxy acid, carrying out treatment at 50-90 DEG C for 0.5-3 hours and carrying out filtration and washing, thus obtaining cellulose, wherein 0.1-0.4mol of hydrogen peroxide is added to 1g of materials and the material concentration is 10-100g / L. The process combines steam explosion, weak alkalis, organic solvents and the method of generating peroxy acid by catalyzing carbonic ester by hydrogen peroxide. The whole process is clean, environmentally friendly and economically feasible.

The invention provides a bacteriophage genome DNA (deoxyribonucleic acid) extraction kit and method. The kit comprises a reagent A (chloroform), a reagent B (DNase I), a reagent C (RNase A), a reagent D (precipitation buffer solution), a reagent E (pyrolysis buffer solution), a reagent F (primary pyrolysis main solution), a reagent G (secondary pyrolysis solution), a reagent H (impurity removal solution), a reagent I (DNA combination buffer solution), a reagent J (rinsing buffer solution) and a reagent K (DNA eluate). The extraction method comprises the following steps: removal of bacterium cells and fragments, degradation of bacterium nucleic acid, bacteriophage particle precipitation, bacteriophage capsid protein structure damage and hydrolysis, impurity removal, DNA liquid separation, DNA adsorption, cleaning of adsorbed DNA and elution of adsorbed DNA. The kit and method can be widely used for extracting the bacteriophage genome DNA, and has the advantages of high extraction speed, high extracted DNA yield and high purity, and the extracted DNA can not be polluted by the host bacterium genome DNA.

The invention discloses a lithocarpus litseifolius polysaccharide extract with high antioxidant activity and an extraction method thereof. The IC50 value of the DPPH free radical scavenging activity of the polysaccharide extract is 0.302 mg / mL, and the IC50 value of the ABTS free radical scavenging activity of the polysaccharide extract is 0.815 mg / mL. The extraction method comprises the following steps: (1) pretreating lithocarpus litseifolius to obtain lithocarpus litseifolius powder; (2) removing fat-soluble compounds in the lithocarpus litseifolius powder; (3) carrying out microwave extraction by taking a DES deep-eutectic solvent as an extraction solvent; and (4) centrifuging the extract and taking the supernatant to obtain thelithocarpus litseifolius crude polysaccharide extract. The polysaccharide is extracted from the lithocarpus litseifolius by adopting a deep-eutectic solvent-microwave-assisted extraction method, so that the dissolving capacity of a solvent to the polysaccharide is effectively improved, the polysaccharide is efficiently extracted, and the prepared lithocarpus litseifolius polysaccharide has high antioxidant activity and has the advantages of high extraction efficiency, low extraction cost, simple extraction process and stable and reliable performance, and the extracted lithocarpus litseifolius polysaccharide has high antioxidant activity and the like.

The invention relates to a method for crudely extracting saccharomyces cerevisiae glutathione, which comprises the following steps: (1) adjusting the pH value of saccharomyces cerevisiae solution by using an acetic acid-hydrochloric acid system to between 3.3 and 3.5; (2) stirring and heating the saccharomyces cerevisiae solution to between 69 and 72 DEG C, preserving the heat for certain time and extracting, and then cooling the solution to the room temperature; and (3) centrifugalizing the solution to remove saccharomyces, and adjusting the pH of the filtered solution to between 2.0 and 2.5 by using concentrated acid, wherein preferably, the material-liquid ratio of the gram of the dried saccharomyces cerevisiae in the saccharomyces cerevisiae solution to the milliliter of deionized water is 1 to 7.5 to 1 to10; the pH is adjusted to between 3.5 and 3.7 by adding acetic acid, and the pH is adjusted to between 3.3 and 3.5 by adding hydrochloric acid; the preservation time is 12 to 17 minutes, and the cooling step adopts ice-water bath; the centrifugal rotating speed is 5,000 to 6,000rpm, and the time is 10 to 15 minutes; and the concentrated acid is concentrated phosphoric acid. The method also comprises step (4) of performing micro-filtration and ultra-filtration. The method has the advantages of skilful design, high extracting yield, high extraction purity, mild condition, easy operation, low cost, light environment pollution, and suitability for industrialized large-scale production.

The invention relates to the technical field of radix astragali medicine extraction, in particular to a process for producing astragalus polysaccharide by using an ultrasonic technology. The process comprises the steps of washing, drying and smashing dry radix astragali, adding water, performing extraction for 0.5 to 1.5h for three times under the condition that the ultrasonic power is 200 to 280W, and the temperature is 60 to 70 DEG C, combining extracting solutions, standing for cooling, adding a natural plant extract clarifying agent KBT-ZTC, standing and then performing rotary evaporation and concentration on a crude extract to obtain an extract crude product; adding ethyl alcohol into the extract crude product, stirring the extract to dissolve, performing static settlement, centrifuging to remove liquid supernatant to obtain precipitate, adding ethyl alcohol again, stirring and centrifuging to obtain pure astragalus polysaccharide. According to the process, electromagnetic energy is converted into thermal energy by using ultrasonic waves to enable a cell membrane to break, intracellular matters such as the astragalus polysaccharide are released and dissolved out, and an introduced clarification and purification process can effectively remove macromolecule impurities such as protein, nucleic acid and starch in the astragalus polysaccharide.

The invention discloses a method for extracting total flavones in orange peel with microwave assisting a complex enzyme, and belongs to the technical field of extraction of effective components of agricultural and sideline products. The method includes the concrete steps that fresh orange peel is washed, dried, smashed and screened through a 60-mesh screen, the obtained orange peel powder is subjected to microwave-assisted processing, the ratio of material to liquid is 1:20, ethanol concentration is 70-73%, the microwave power is 240w, the microwave time is 60 s, and the number of times of extraction is three; the complex enzyme is added to the sample obtained after microwave-assisted processing for further extraction, the sample is cooled to room temperature, then the pH value is adjusted to be 5.0, cellulase of 4-5% and pectinase of 4-5% are added, and extraction is carried out for 1-1.2 h at the temperature of 50 DEG C to complete the whole extraction process of orange peel flavones; rotary evaporation, macroreticular resin purification and freeze drying are carried out on a solution obtained after filtration to obtain an orange peel flavones product. The content of the total flavones extracted from the orange peel reaches 39.5 mg / g, and the method is easy to operate, high in extraction rate and good in product quality.

The invention relates to a dendrobium candidum skin moisturizer and a preparation method thereof. A dendrobium candidum skin moisturizer capable of retaining effective components in dendrobium candidum and a preparation method of the dendrobium candidum moisturizer are not available. The dendrobium candidum moisturizer comprises 8.0-10.0 parts by weight of filtered dendrobium candidum extract liquor. The filtered dendrobium candidum extract liquor contains polysaccharide, chlorophyll, vitamins and proteins. The preparation method of the dendrobium candidum skin moisturizer sequentially comprises the following processes: A, pretreatment process; B, low-temperature superfine grinding process: putting water with temperature of 2-10 DEG C and cut dendrobium candidum into a homogenizer, wherein the weight ratio of the dendrobium candidum to the water is (1:6) to (1:8); starting the homogenizer and controlling the discharge material fineness to be below 200-300 microns, so as to obtain the dendrobium candidum extract liquor; C, filtering process; and D, emulsification process. Fresh dendrobium candidum is taken as a raw material to carry out low-temperature extraction, and the effective components of dendrobium candidum can be preserved as much as possible.

The application of the invention discloses a reprocessing method for improving the quality of semi-finished tea or finished tea. According to the method disclosed by the application of the invention, enzymolysis treatment is performed on the semi-finished tea or finished tea by using a compound enzyme preparation, and then high-temperature baking and low-temperature baking are performed so that tea leaves with improved flavor and high leaching rate are obtained; the compound enzyme preparation after being subjected to the enzymolysis treatment consists of A enzymes and B enzymes in a weight ratio of the A enzymes to the B enzymes being (3-8) to (1-5), the A enzymes comprise at least one of plant composite hydrolases, cellulase and hemicellulase, and the B enzymes comprise at least one of tannin enzyme, flavored protease, beta-glucosidase, beta-primeverosidase and laccase; and the steps of high-temperature baking and low-temperature baking comprise the step of performing short-time high-temperature treatment and then performing low-temperature baking until a complete drying state is obtained. Through the adoption of the method disclosed by the application of the invention, the distinctive fragrance type of high-fire fragrance and caramel fragrance of tea leaves is effectively reflected, the number of reducing sugar substances having sweet and mellow taste and sweetness after taste in tea soup is increased, the bitter taste is alleviated and reduced, the problems that the fragrance is low and the taste is light and bitter are solved, and the whole quality of the tea leaves is improved.

The present invention discloses a pipless fructus momordicae and the cultivation method. The cultivation method is: 1) breeding the tissue cultivation seedling with the male and the female ex-plant of diploid fructus momordicae; 2) doubly introducing the chromosome with the tissue-cultivation successive-transfer seedling; 3) performing the differential cultivation with different parts of the maleand the female plants after introducing treatment; 4) cutting the stem tip and the stem section of the sprout differentially cultivated to perform the gemmulation cultivation; 5) examining the numberof the chromosome of a cultivated entire plant to select the tetraploid plant for performing the root cultivation and the seedling exercise; 6) planting the tetraploid plant and the diploid plant according to the conventional techniques and performing the artificial pollination to get the hybrid seed when in flower; 7) breeding the hybrid seed to be a complete plant and examining the number of the chromosome; selecting the triploid plant to perform the root cultivation and seedling exercise; 8) planting the triploid plant and the diploid plant and performing the artificial pollination on the male diploid plant with the female triploid plant when in flower; the pipless fructus momordicae can be obtained after the fruiting of the female plant.

The invention relates to an efficient and energy-saving extraction tank. The extraction tank comprises a tank body, a microwave ultrasonic generator and a liquid injection overflow mechanism which arearranged on the wall of the tank, a solid and liquid material separating and converging circulation channel in the tank, and a filter type spiral conveyor below the tank. When the tank is operated, the slurry flat turn circulation flow in the tank is changed into solid and liquid material upper-converting and lower-separating ascending and descending flow, so that microwave ultrasonic wave can perform targeted radiation on a solid material but not on a liquid material, the special structure of the tank allows liquid injection overflow and repeated retraction, and operations of liquid discharging and deslagging can be accomplished at the same time during discharging. The tank is obvious in energy-saving and time-saving effect, low in operation cost and high in efficiency, and provides a novel equipment selection for enterprise production.

The invention relates to a yield increasing method for propolis with supercritical CO2 extraction, which comprises the following steps: fine riversand and propolis are mixed evenly in certain proportion and then are put in extraction kettle; the supercritical CO2 fluid extraction device is started according to the operational instruction; the mixture is extracted based on the set temperature, pressure, time and CO2 flow to obtain aromatic propolis in light yellow color. The method is not only suitable for blocky proposes, sand proposes and dispending proposes, but also suitable for the propolis residues after ethanol extraction method. The method has the advantages that: firstly, the material of riversand is available easily, low in cost and has no pollution; secondly, the propolis has uniform distribution, stable structure and does not collapse due to the reticular framework support structure; thirdly, the yield of propolis is hight; fourthly, the technology is simple and the production cost is low.

The invention relates to a device for preparing foodstuffs, comprising: -a rotary cutting tool (30) having a side wall (32) having at least one external cutting member (34) adjacent to a through-passage (35), the side wall (32) forming a front opening for evacuating pieces of cut foodstuffs, -a casing (20) defining a housing (21) having a mounting opening for fitting the cutting tool in the housing (21), the casing (20) having a hollow shaft (23) for introducing the foodstuffs to be cut, the hollow shaft (23) opening into the housing (21) at least partially next to the or at least one of the external cutting members (34). In accordance with the invention: -the casing (20) bears a duct (71) extending the housing (21), -and the cutting tool (30) rotates a pressing member (40) having at least one blade (42) that presses the cut pieces of foodstuffs against at least a portion of filtering wall (51) formed in the duct (71), in order to obtain juices and / or coulis.

The invention discloses a preparation method of rich-fragrance instant cold-extract coffee powder. The preparation method of the rich-fragrance instant cold-extract coffee powder comprises the following steps: 1) baking raw coffee beans so as to allow emission of fragrance; 2) grinding the cooked coffee beans subjected to the emission of the fragrance so as to obtain coffee bean granules; 3) putting the coffee bean granules subjected to the grinding into a column-type insulated high-pressure extraction tank, and carrying out staged extraction so as to separately collect first extraction liquidand last extraction liquid; 4) filtering the first solution by using a refined filtration membrane for standby application; 5) centrifuging the last extraction liquid, and carrying out filtration andconcentration so as to obtain concentration liquid; 6) mixing the concentration liquid obtained in the step 5) with the first solution filtered in the step 4) so as to obtain a mixed liquid; 7) laying the mixed liquid in a plate, and carrying out freeze-drying; and 8) taking the freeze-dried product out from an oven, and carrying out crushing and filtering so as to obtain the rich-fragrance instant cold-extract coffee powder. The preparation method of the rich-fragrance instant cold-extract coffee powder is low in temperature in the whole process, so that the freeze-dried product has very excellent solubility; and moreover, the first extraction liquid and the last extraction liquid are separately collected in the step of extraction, so that flavor substances of the product are preserved to the maximum extent with production cost reduced and production capacity improved.

The invention discloses a high-yield preparation method for high-purity low-molecular-weight lignin and belongs to the field of biochemical engineering. According to the method, non-grain crops or bioethanol wood fiber waste is adopted as raw materials, the raw materials are pretreated through dilute acid steam explosion, and steam explosion materials with hemicellulose removed are obtained; the steam explosion materials and an ethanol water solution are added to a high-pressure reactor, temperature rises to 100 DEG C to 300 DEG C, and reacting is carried out for 0.5 h to 5 h at the temperature; after reacting is finished, cooling is carried out, products are dissolved in a reaction solution, pH is adjusted to be 1-7, and the high-purity low-molecular-weight lignin is obtained. The method has the advantages of being economical and feasible, environmentally friendly and high in yield and efficiency, and has wide prospects in the high-valued utilization direction of the lignin in agricultural waste.

The invention relates to a method for extracting pepper oleoresin through ultrasonic continuous countercurrent. The method comprises the following steps: (1) well-chosen black / white pepper dry fruits are smashed to pepper particles to pass through a screen with meshes of 20 to 80, and the screened pepper particles are conveyed in a pre-gumming cage for pre-gumming; (2) the pre-gummed pepper particles are passed in an ultrasonic countercurrent leaching device for extracting; extraction solvent is alcohol with concentration of 65-95%; the weight ratio of the pepper particles to the solvent is (1:5) to (1:10); the ultrasonic frequency range is 10-100 KHz; the leaching temperature is kept in a range of 25-40 DEG C; and the leaching time is 30-90 minutes; and (3) the leached alcohol leachate is passed in a film evaporator for reducing pressure, vaporizing and desolventizing; and obtained concentrate is the pepper oleoresin. The method can improve the dissolution rate of the pepper resin in peppers, shortens the extraction time, reduces the solvent dosage, is easy to operate and is low in labor intensity; and the pepper oleoresin obtained through extraction has excellent dissolution characteristic and dispersity.

The invention discloses a method for rapidly extracting long-fragment DNA from fresh fungus mycelia. The method comprises the following steps: thallus preparation: extracting fresh cultured fungus mycelia, carrying out centrifugation to remove moisture, weighing the mycelia, and rapidly grinding the mycelia into powder in liquid nitrogen; cell lysis: adding a DNA extracting buffer solution, adding60 mu L of 20mg / mL proteinase K, uniformly mixing, and carrying out water bath at 65 DEG for 30 minutes; DNA extraction: adding 0.33mL of KAc, carrying out water bath for 20 minutes, carrying out extraction by virtue of a mixture of phenol, chloroform and isoamyl alcohol, carrying out centrifugation, extracting supernate, adding absolute ethyl alcohol for precipitation, standing at a low temperature for 30 minutes, carrying out centrifugation, and collecting precipitates; purification: adding RNaseA, processing at 37 DEG C for 30 minutes, respectively extracting by virtue of a mixture of phenol, chloroform and isoamylol in a ratio of 25 to 24 to 1 and a mixture of chloroform and isoamylol in a ratio of 24 to 1, and carrying out low-temperature centrifugation for 5 minutes; and extractingsupernate, adding absolute ethyl alcohol for precipitation, selecting precipitates, rinsing, carrying out air drying, dissolving the precipitates into a TE buffer solution, and carrying out preservation for later use. The DNA amount of filamentous fungi extracted by virtue of the method is increased twice, the concentration of the filamentous fungi reaches 277ng / mu L, the lengths of fragments aremore than or equal to 20000bps, and the filamentous fungi are not degraded and are high in purity.

The invention relates to a method for extracting semen nigellae oil by using supercritical CO2, belonging to the field of food processing and specially used for comprehensive development and deep processing of semen nigellae. Taking semen nigellae (Semen Nigellae) as a raw material, the method comprises the following process steps: semen nigellae (Semen Nigellae) is dried and crushed and is extracted by supercritical CO2, and the optimal process parameters areas follows: the extraction pressure is 29.20 MPa, the extraction temperature is 40.15 DEG C and a CO2 flow is 21.92 L / h. The semen nigellae oil extracted under the conditions has the purity of above 99 percent and the maximum extraction yield of 37.897 percent. The process for extracting the semen nigellae oil by using the supercritical CO2 provides a new method for deep processing of the semen nigellae and can efficiently carry out research on extracting the semen nigellae oil by the supercritical CO2. The invention has the advantages of strong operability, favorable safety, higher yield, favorable repeatability, high extraction efficiency, simple process, high product purity, non pollution and the like and can be used for extracting the semen nigellae oil in production.

The invention relates to a supercritical carbon dioxide extraction method of sea-buckthorn seeds, and the method is used for extracting sea-buckthorn seed oil from raw sea-buckthorn seed materials. The method comprises the following steps: drying the raw sea-buckthorn seed materials, removing impurities from the raw sea-buckthorn seed materials, breaking the hulls of the raw sea-buckthorn seed materials and extracting the sea-buckthorn seed oil, wherein the husks of the hulled raw sea-buckthorn seed materials are partially connected, the inner kernels of the hulled raw sea-buckthorn seed materials are opened and exposed, the temperature of the sea-buckthorn seeds is not higher than 50 DEG C during a hull breaking process, and the loose stacking density of the hulled raw sea-buckthorn seed materials is 1.25-1.3 times that of the unhulled raw sea-buckthorn seed materials. The method disclosed by the invention has the beneficial effects of reducing the temperature of the raw sea-buckthorn seed materials during the hull breaking process and guaranteeing the quality of the sea-buckthorn seed oil; the hulled raw sea-buckthorn seed materials are fluffy and the natural stacking volume is increased and thus the sea-buckthorn seeds are in full contact with an extracting medium during the supercritical carbon dioxide extraction process of the sea-buckthorn seeds, so that the extraction yield is increased, the extraction period is shortened and a remarkable benefit is achieved.

The invention relates to the radix astragali medicine extraction technology field, and concretely provides a technology for producing radix astragali polysaccharide by utilization of an alkaline process extraction technology. The method comprises steps: dried radix astragali roots are taken, cleaned, dried, crushed, added with an alkaline hydrolysis liquid, and soaked for 2-3h at a constant temperature, extraction is repeated for three times, the extracting solutions are merged and the mixture is allowed to stand for cooling, a natural plant extracting solution clarificant KBT-ZTC is added, the crude extracting solution is condensed through rotary evaporation after standing and an extract crude product is obtained; ethanol is added in the extract crude product, the extract is stirred and dissolved, the mixture is allowed to stand for precipitation, centrifugation is carried out, the supernatant is removed and sediments are obtained; ethanol is added again, stirring and centrifugation are carried out and a pure product radix astragali polysaccharide is prepared. Based on a normal water extraction alcohol precipitation method, the plant traditional Chinese medicine clarification technology is introduced to achieve extraction and purification of high-quality radix astragali polysaccharide. The extraction yield of active ingredients in radix astragali polysaccharide is raised, and the radix astragali polysaccharide extraction rate of the improved method is more than 10.6%, the highest being 13.5%.