Lithocarpus litseifolius polysaccharide extract with high antioxidant activity and extraction method thereof
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A polysaccharide extraction and extraction method technology, applied in the field of plant polysaccharide extraction, can solve the problems of low yield, unsatisfactory antioxidant activity, etc., and achieve the effects of low extraction energy consumption, stable and reliable extraction process, and simple extraction process
Active Publication Date: 2021-08-06
INST OF URBAN AGRI CHINESE ACADEMY OF AGRI SCI +1
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[0006] Existing methods for extracting polysaccharides from sweet tea all have de
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Embodiment 1
[0040] The extraction of embodiment 1 sweet tea polysaccharide
[0041] (1) Dry the finished sweet tea in a constant temperature hot air oven at 45°C to constant weight, then grind it with a Super Mill-1500 powder machine, then pass through a 60-mesh sieve, put the sweet tea powder into a sealed bag, and store at -20°C spare.
[0042] (2) Accurately weigh 2.0g of sweet tea powder, add it to a 50mL centrifuge tube, and vortex to mix, add 20mL of 80% ethanol (1:10, w / v), use PL-S80T ultrasonic cleaner at 640W power Ultrasound for 30min. After the sample is balanced, it is centrifuged at 4000g for 10 minutes by an X3R high-speed refrigerated centrifuge. After the supernatant is discarded, the precipitate is added to 20 mL of 80% ethanol, and the PL-S80T ultrasonic cleaner is used to sonicate at 80% power for 30 minutes. After the sample is balanced, Centrifuge at 4000 g for 10 min in an X3R high-speed refrigerated centrifuge, and discard the supernatant to retain the precipitat...
Embodiment 2
[0051] The extraction of embodiment 2 sweet tea polysaccharides
[0052] The difference with Example 1 is that in step (3) choline chloride-ethylene glycol: ultrapure water=8:2 (v:v), the extraction time in step (4) is 10min, and the microwave power is 560W ; All the other are identical with embodiment 1.
[0053] After calculation, the polysaccharide extraction rate of this embodiment is 3.73%, and the polysaccharide extraction yield is 74.6mg.
Embodiment 3
[0054] The extraction of embodiment 3 sweet tea polysaccharides
[0055] The difference from Example 1 is that the extraction time in step (4) is 10min, and the microwave power is 640W; the rest are the same as in Example 1.
[0056] After calculation, the extraction rate of this embodiment is 3.44%, and the extraction yield of polysaccharide is 65.8mg.
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Abstract
The invention discloses a lithocarpus litseifolius polysaccharide extract with high antioxidant activity and an extraction method thereof. The IC50 value of the DPPH free radical scavenging activity of the polysaccharide extract is 0.302 mg/mL, and the IC50 value of the ABTS free radical scavenging activity of the polysaccharide extract is 0.815 mg/mL. The extraction method comprises the following steps: (1) pretreating lithocarpus litseifolius to obtain lithocarpus litseifolius powder; (2) removing fat-soluble compounds in the lithocarpus litseifolius powder; (3) carrying out microwave extraction by taking a DES deep-eutectic solvent as an extraction solvent; and (4) centrifuging the extract and taking the supernatant to obtain thelithocarpus litseifolius crude polysaccharide extract. The polysaccharide is extracted from the lithocarpus litseifolius by adopting a deep-eutectic solvent-microwave-assisted extraction method, so that the dissolving capacity of a solvent to the polysaccharide is effectively improved, the polysaccharide is efficiently extracted, and the prepared lithocarpus litseifolius polysaccharide has high antioxidant activity and has the advantages of high extraction efficiency, low extraction cost, simple extraction process and stable and reliable performance, and the extracted lithocarpus litseifolius polysaccharide has high antioxidant activity and the like.
Description
technical field [0001] The invention relates to a polysaccharide extract extracted from plants and an extraction method thereof, in particular to a polysaccharide extract with high antioxidant activity extracted from sweet tea and an extraction method, belonging to the field of plant polysaccharide extraction. Background technique [0002] Lithocarpus litseifolius (Hance) Chun (scientific name: Lithocarpus litseifolius (Hance) Chun) is an arbor of the Fagaceae family and is distributed in various provinces and regions south of the southern slope of the Qinling Mountains in China. In 2017, Mujiang Yeke was approved as a new food raw material. Because the young leaves of Mujiang Ye Ke have a sweet taste and are sticky when chewed, most mountain residents in the south of the Yangtze River use their leaves as a tea substitute, commonly known as sweet tea. Sweet tea has been eaten in China for thousands of years. It is a plant that can be used as medicine and food, and has three...
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IPC IPC(8): C08B37/00A61P39/06
CPCC08B37/0003A61P39/06Y02A50/30
Inventor 甘人友郭欢刘宏艳刘毅何晓琴夏宇
Owner INST OF URBAN AGRI CHINESE ACADEMY OF AGRI SCI
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