Preparation method of high-purity collagen protein sponge

A collagen sponge and collagen technology, applied in the direction of animal/human protein, connective tissue peptide, specific peptide, etc., can solve the problems of long production cycle of collagen sponge, poor hemostatic performance, low yield, etc., and shorten the production cycle , short time, high yield effect

Inactive Publication Date: 2014-05-07
HARBIN INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a preparation method for high-purity collagen sponge in order to solve the problems of long production cycle, low yield, low purity and poor hemostatic performance in the collagen sponge prepared by the existing method

Method used

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  • Preparation method of high-purity collagen protein sponge
  • Preparation method of high-purity collagen protein sponge
  • Preparation method of high-purity collagen protein sponge

Examples

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specific Embodiment approach 1

[0030] Specific embodiment one: the preparation method of the high-purity collagen sponge of the present embodiment is carried out according to the following steps:

[0031] 1. Pretreatment of fresh beef Achilles tendon: remove fascia and grease from 100-250g fresh beef Achilles tendon, wash and cut into small pieces of 0.5cm×0.5cm, put them into a tissue masher, add 300-500ml Distilled water, turn on the machine and mash, then wash with distilled water, then soak in 1000ml of sodium carbonate solution with a mass concentration of 0.05% to 3% for 10 to 24 hours, filter, and dry the precipitate in a natural state;

[0032] 2. Extraction of collagen: put 5-50g of dried bovine Achilles tendon into a three-necked flask, add 500-1500ml of acetic acid with a mass concentration of 0.1%-5%, stir to dissolve it, and then add 100-500ml containing 0.5 ~5g of pepsin or ficin with 0.1%~5% acetic acid in mass concentration, and then placed in a constant temperature water tank at 5°C, stirre...

specific Embodiment approach 2

[0045] Specific embodiment two: the difference between this embodiment and specific embodiment one is that in step one, use 1000ml mass concentration of 0.8% sodium carbonate solution to soak for 12h. Other steps and parameters are the same as those in Embodiment 1.

specific Embodiment approach 3

[0046] Specific embodiment three: what this embodiment is different from specific embodiment one or two is that in step 2, the bovine Achilles tendon after 25g drying is placed in the there-necked flask, adding 800ml mass concentration is the acetic acid of 1.2%, stirring makes it dissolve, Then add 500ml of acetic acid with a mass concentration of 1.2% containing 3.5g of pepsin or ficin, and then place it in a constant temperature water tank at 5°C with even intermittent stirring and extract for 3.5 days. Other steps and parameters are the same as those in Embodiment 1 or Embodiment 2.

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Abstract

The invention provides a preparation method of high-purity collagen protein sponge, and relates to a preparation method of collagen protein sponge. The preparation method of the high-purity collagen protein sponge is used for solving the problems that the collagen protein sponge prepared by using a conventional method is long in production cycle and low in yield and purity and has poor hemostasis performance. The preparation method of the high-purity collagen protein sponge comprises the following steps: step one. pretreating fresh bovine heel tendons; step two. extracting collagen protein; step three. centrifuging; step four. salting out; step five. dissolving; step six. carrying out gradient dialysis; step seven. pre-freezing; step eight. carrying out freeze drying; and step nine. sterilizing. The final product prepared by using the method has a smooth and flat surface and relatively good hemostatic performance and is uniform in pore size distribution. The product has relatively high purity (the total amount of amino acids reaches 97.73%), an obvious hemostatic effect and no abnormal taste, is safe, non-toxic, high in yield and short in time; liquid is clear without impurities; the production cycle is shortened; the whole preparation process is carried out at a room temperature; the biological activity of the collagen protein is maintained; and the application of the high-purity collagen protein sponge in clinical is improved.

Description

technical field [0001] The invention relates to a preparation method of a collagen sponge. Background technique [0002] Collagen is the most abundant protein in mammals, accounting for 25% to 30% of the total protein in the body, equivalent to 6% of body weight, and exists in almost all tissues. It is an extracellular matrix protein and exists in the form of insoluble fibers , with high tensile strength, is very important to the formation of animal and human skin, blood vessels, bones, muscles and bones, and cartilage. It is an important substance of connective tissue and the main factor determining the toughness of connective tissue. Collagen is a protein family with both common features and differences in structure. At present, 27 different types of collagen have been found, which are called type I collagen, type II collagen, type III collagen, etc. according to the sequence of discovery. , with uppercase Roman numerals for naming. Collagen is also composed of amino aci...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J9/28C08L89/00C12P21/06C07K14/78C07K1/36C07K1/34C07K1/30
Inventor 黄玉东李大龙贺金梅程玮璐吴亚东王承旭
Owner HARBIN INST OF TECH
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