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Selenium-riched protein and selenium-riched amino acid, prepn. method and use thereof

A technology of selenium-enriched amino acid and protein, which is applied in the field of selenium-enriched protein and selenium-enriched amino acid and its preparation, can solve the problems of rare products and achieve low raw material cost, simple process flow, and low temperature and pressure requirements Effect

Inactive Publication Date: 2005-01-05
HUAZHONG UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The purpose of the present invention is to aim at the health products that have not obtained selenium-enriched protein and selenium-enriched amino acids from selenium-enriched silkworm chrysalis in the current market, and the products of organic selenium are also rare in the market

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] The preparation of embodiment 1 selenium-enriched protein

[0038] Rinse 100g of selenium-enriched fresh silkworm chrysalis, dry them naturally until they are 80% dry, crush them with a pulverizer, extract and degrease them with 300mL of petroleum ether to obtain degreased silkworm chrysalis, then add 360mL of 1% sodium hydroxide, stir at 30°C to dissolve silkworm chrysalis protein 10 Hours, after filtering with polyester cloth, the residue was washed with a small amount of water, and the filtrate was collected. The filtrate was decolorized and deodorized with 0.5g activated carbon for 5h, and then filtered with 6mol.L -1 Adjust the pH of the filtrate to 4.5 with HCl, let it stand overnight at 4°C, and precipitate the protein the next day, centrifuge at 4000 rpm, and freeze-dry the precipitate to obtain the selenium-enriched protein. Quality inspection results: the protein yield in the dry pupa is 45.3%, and the selenium content in the protein is 13.5mg Se / kg protein....

Embodiment 2

[0039] The preparation of embodiment 2 selenium-enriched protein

[0040] Rinse 100g of selenium-enriched fresh silkworm chrysalis, dry them to 80% dryness, pulverize them with a pulverizer, extract and degrease them with 300mL of petroleum ether to obtain degreased silkworm chrysalis, then add 300mL of 2.5% sodium hydroxide, stir and dissolve silkworm chrysalis protein at 30°C for 10 hours , After filtering with polyester cloth, the residue was washed with a small amount of water, and the filtrate was collected. The filtrate was decolorized and deodorized with 0.8g activated carbon for 5h, and then filtered with 6mol.L -1 Adjust the pH of the filtrate to 4.5 with HCl, let it stand overnight at 4°C, and precipitate the protein the next day, centrifuge at 4000 rpm, and freeze-dry the precipitate to obtain the selenium-enriched protein. Quality inspection results: the protein yield in the dry pupa is 48.5%, and the selenium content in the protein is 15.0 mg Se / kg protein.

Embodiment 3

[0041] The preparation of embodiment 3 selenium-enriched amino acids

[0042] Take 10g of selenium-enriched protein powder, add 120mL of water to dissolve, then add 0.1g of trypsin and 0.2g of papain at the same time, and hydrolyze at 30°C for 10 hours under stirring. Centrifuge at 2500 rpm for centrifugal filtration, and the residue is hydrolyzed in the same way as above. Repeat the above process twice until all the selenium-enriched proteins are dissolved to obtain a selenium-enriched amino acid solution, wherein the selenium content is 1.17 μg Se / mL. Amino acid powder can be obtained by spray drying (≤60°C).

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Abstract

A Se-enriched protein and a Se-enriched amino acid are prepared from Se-enriched fresh silkworm chrysalis through drying, deoiling, extracting protein in diluted alkali solution, regulating pH value to educe Se-enriched protein, centrifugal treating, depositing, freeze drying to obtain powder, and hydrolyzing to obtain Se-enriched amino acid. They can be used to prepare food for preventing cancer.

Description

technical field [0001] The invention relates to a selenium-enriched protein and selenium-enriched amino acid prepared from selenium-enriched silkworm chrysalis and a preparation method thereof; and a health food with selenium-enriching and cancer-preventing functions prepared from the selenium-enriched protein and selenium-enriched amino acid. Background technique [0002] Selenium is an essential trace element for living things. Selenium has the functions of enhancing immunity, anti-aging and preventing and treating tumors in the body. Selenium deficiency leads to a decrease in the body's immune function and antioxidant capacity, and is associated with the occurrence of various diseases. Supplementing an appropriate amount of selenium can improve the body's immunity and prevent various diseases, so it has been widely valued. According to the Chinese Nutrition Society in 1988, the daily dietary selenium supply (RDA) for adults is 50 μg. However, in intervention experiments...

Claims

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Application Information

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IPC IPC(8): A23J1/00A23L33/17A23L33/175
Inventor 刘琼胡德聪徐辉碧韩定献杨祥良
Owner HUAZHONG UNIV OF SCI & TECH
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