Method for extracting propolis by simulative biological fluid bed supercritical CO2 fluid

Abstract

The invention provides a method for extracting propolis by simulative biological fluid bed supercritical CO2 fluid, which comprises that: bran (or rice bran and the like) taken as biomembrane carrier and original gum particles after physical treatment simulate a fluid bed in an extraction kettle to perform gridded filling, the supercritical CO2 fluid is used for extraction, and the lowest speed of a granular bed converting from a stationary state to fluidization is regulated by regulating temperature, pressure and flow rate of the fluid in the extraction process so as to reach the critical fluidization state; therefore, high-quality and high-yield propolis extract is obtained through a cyclic process of fluidization diffusion, dissolution and separation between granules and the fluid. The method improves the yield of the propolis extract, reduces the cost, and extracts nearly all terpene compounds and 40 to 50 percent of flavonoid compounds in the propolis at the same time; and products do not have organic solvent.

Description

technical field [0001] The invention relates to an extraction method of active ingredients in propolis, in particular to a simulated biological fluidized bed supercritical CO 2 A method for fluid extraction of propolis belongs to the technical field of propolis extraction methods. Background technique [0002] Propolis (propolis) is the resinous substance secreted by the worker bees in the bee colony from the young shoots and callus of poplar, willow, pine and other plants with their own digestive glands and wax glands and other glandular secretions (such as beeswax). and various digestive enzymes) to form a viscous jelly. The composition of propolis is very complex, and more than 300 substances have been isolated and identified, among which flavonoids, terpenes, aliphatic and aromatic acids and their esters have obvious health functions on the human body, such as antibacterial and anti-inflammatory , anti-virus, lowering blood sugar and blood lipids, enhancing immune regu...

AI Technical Summary

Problems solved by technology

Therefore, in supercritical CO 2 Affected by factors such as temperature and pressure during the fluid extraction process, propolis is prone to compaction in the extraction kettle, resulting in CO 2 The gas passes through the extract in a diffused form into a channel through the extract, thereby reducing the contact area with the extract in a large range, so that the effective components of the extracted propolis are reduced, the yield is reduced, and the extraction fails.

[0006] In order to increase the yield of propolis, a Chinese patent document discloses a method for extracting propolis. The patent number of this document is "200810046890.4", and the title of the patent is "a method for improving supercritical CO 2 Method for Propolis Extraction Yield", the steps of the method are as follows: rinse the fine river sand below 10 meshes and dry it for later use, take the raw propolis and freeze it at low temperature and crush it into granules. The ratio is evenly mixed, placed in the extraction material preparation barrel, and then placed in the extraction kettle, and the supercritical CO is started according to the operating procedures. 2 The extraction device extracts propolis according to the set temperature, pressure, time and flow rate. The propolis yield of this method can generally reach about 15%. Although the yield reaches 15%, it is not too high and the fine river sand is not easy. and poor viscosity, thus increasing the production cost and making the process more cumbersome