Method for extracting total flavones in orange peel with microwave assisting complex enzyme

Abstract

The invention discloses a method for extracting total flavones in orange peel with microwave assisting a complex enzyme, and belongs to the technical field of extraction of effective components of agricultural and sideline products. The method includes the concrete steps that fresh orange peel is washed, dried, smashed and screened through a 60-mesh screen, the obtained orange peel powder is subjected to microwave-assisted processing, the ratio of material to liquid is 1:20, ethanol concentration is 70-73%, the microwave power is 240w, the microwave time is 60 s, and the number of times of extraction is three; the complex enzyme is added to the sample obtained after microwave-assisted processing for further extraction, the sample is cooled to room temperature, then the pH value is adjusted to be 5.0, cellulase of 4-5% and pectinase of 4-5% are added, and extraction is carried out for 1-1.2 h at the temperature of 50 DEG C to complete the whole extraction process of orange peel flavones; rotary evaporation, macroreticular resin purification and freeze drying are carried out on a solution obtained after filtration to obtain an orange peel flavones product. The content of the total flavones extracted from the orange peel reaches 39.5 mg / g, and the method is easy to operate, high in extraction rate and good in product quality.

Description

technical field [0001] A method for extracting total flavonoids from navel orange peels with microwave-assisted compound enzymes, which belongs to the technical field of extraction of active ingredients of agricultural and sideline products, and specifically relates to a treatment technology for extracting flavonoids by a microwave compound enzyme method, and finally obtains total flavonoids with a content of more than 39.5 mg / g. Flavonoid products provide a basis for the extraction method of flavonoids. Background technique [0002] Navel orange (Citrus sinensis Osbeck), an evergreen tree belonging to the genus Citrus of Rutaceae, is an important fruit resource in my country and even in the world. Regular consumption can reduce fat, beautify the skin, and fight cancer. It has become the "king of citrus" in the world's fresh food varieties. Therefore, the navel orange industry has good application prospects and high economic benefits. However, the actual utilization rate o...

AI Technical Summary

Problems solved by technology

Alcohol extraction has the advantages of simple process, strong operability, avoiding heating, and less energy consumption, but the extraction cycle is long and solvent consumption is relatively large; supercritical fluid extraction has the advantages of fast extraction speed, high efficiency, simple operation, and low energy consumption. consumption, inorganic solvent residues, etc., but the requirements for equipment are extremely high, which is not conducive to industrial production

For example, the patent "Microwave-assisted extraction method of total flavonoids from citrus peels" (publication number: CN104306501A, published on January 28, 2015) discloses a microwave-assisted extraction method of total flavonoids from citrus peels. This method has simple process and low cost , but the simple use of microwave extraction power is large, the time is long, and the active ingredients of flavonoids are easily destroyed; ) discloses a method for preparing citrus flavonoids from citrus peels. This method adopts the method of reflux to prepare citrus flavonoids, which requires high equipment and requires a large ethanol concentration, which makes energy waste more serious