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A kind of ethyl sulfate artificial antigen, preparation method and application

A technology of ethyl sulfate and artificial antigen, which is applied in the direction of preparation of sulfate ester, preparation method of peptide, chemical instrument and method, etc., can solve the problem of poor mastery, affecting the accuracy of detection and inspection results, and affecting the accuracy of alcohol inspection results, etc. problems, to achieve the effect of few influencing factors, high accuracy and accurate detection results

Active Publication Date: 2022-06-07
HANGZHOU BIOTEST BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are many problems with the breath alcohol detector, which affect the accuracy of the alcohol test results
Because there are many uncertain factors affecting the test results of the breath alcohol detector, for example, the breath alcohol detection technology requires the collection of the gas exhaled from the deep lungs of the human body, so the way of exhalation, the length of time, and the air flow rate The size and the degree of diffusion of exhaled breath in the air will affect the accuracy of the test; for example, the breath alcohol detector needs to exhale deeply for 2.5 seconds, which is difficult to master in actual operation; for example, before the breath test, Hyperventilation or high-frequency deep breathing by the driver will reduce the alcohol concentration in the exhaled gas by 20%. If you hold your breath for 20 seconds before the test, the alcohol concentration in the exhaled gas will increase by 15%. Therefore, the driver Breathing patterns can also affect the accuracy of test results

Method used

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  • A kind of ethyl sulfate artificial antigen, preparation method and application
  • A kind of ethyl sulfate artificial antigen, preparation method and application
  • A kind of ethyl sulfate artificial antigen, preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] (1) Preparation of ethyl sulfate hapten

[0037] Weigh 100mg (0.71mmol) of 2-aminoethanol hydrogen sulfate, add it to a 50ml single-necked round bottom flask, then add 5ml of N,N-dimethylformamide (DMF), 276mg (2mmol) of potassium carbonate and 300mg (1.31 mmol) 2-(4-(bromomethyl)phenyl)acetic acid, a stirring bar was added, and the reaction was stirred at room temperature for 20 hours; after the reaction was completed, the solvent was evaporated to dryness under reduced pressure, and purified by thin-layer chromatography, TLC: chromatographic solution It is dichloromethane:methanol=1:1 (volume ratio), product ratio shift value Rf=0.3~0.4; 86mg of hapten (2) is obtained, and the specific synthetic route is as follows figure 1 shown.

[0038] (2) Preparation of ethyl sulfate artificial antigen

[0039] a. Weigh 50mg (0.17mmol) of ethyl sulfate hapten into a 50ml round-bottomed flask, add 2.5ml of N,N-dimethylformamide (DMF), and then add 39mg (0.34mmol) of N-hydroxysuc...

Embodiment 2

[0053] (1) Preparation of ethyl sulfate hapten

[0054] Weigh 100mg (0.71mmol) of 2-aminoethanol hydrogen sulfate, add it to a 50ml one-neck round bottom flask, then add 5ml of N,N-dimethylformamide (DMF), 138mg (1mmol) of potassium carbonate and 200mg (0.87 mmol) 2-(4-(bromomethyl)phenyl)acetic acid, a stirring bar was added, and the reaction was stirred at room temperature for 20 hours; after the reaction was completed, the solvent was evaporated to dryness under reduced pressure, and purified by thin-layer chromatography, TLC: chromatographic solution It is dichloromethane: methanol=1:1 (volume ratio), the product ratio shift value Rf=0.3~0.4; obtain hapten (2) 40mg, the specific synthetic route is as follows figure 1 shown.

[0055] (2) Preparation of ethyl sulfate artificial antigen

[0056] a. Weigh 40mg (0.14mmol) of ethyl sulfate hapten into a 50ml round-bottomed flask, add 2ml of N,N-dimethylformamide (DMF), and then add 24mg (0.21mmol) of N-hydroxysuccinimide Amin...

Embodiment 3

[0063] (1) Preparation of ethyl sulfate hapten

[0064] Weigh 100mg (0.71mmol) of 2-aminoethanol hydrogen sulfate, add it to a 100ml single-neck round bottom flask, then add 10ml of N,N-dimethylformamide (DMF), 552mg (4mmol) of potassium carbonate and 600mg (2.62 mmol) 2-(4-(bromomethyl)phenyl)acetic acid, a stirring bar was added, and the reaction was stirred at room temperature for 20 hours; after the reaction was completed, the solvent was evaporated to dryness under reduced pressure, and purified by thin-layer chromatography, TLC: chromatographic solution It is dichloromethane: methanol=1:1 (volume ratio), the product ratio shift value Rf=0.3~0.4; obtain the hapten (2), the specific synthetic route is as follows figure 1 shown.

[0065] (2) Preparation of ethyl sulfate artificial antigen

[0066] a. Weigh 50mg (0.17mmol) of ethyl sulfate hapten into a 100ml round-bottomed flask, add 10ml of N,N-dimethylformamide (DMF), and then add 1.1mmol of N-hydroxysuccinimide (NHS) ...

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Abstract

The invention discloses an ethyl sulfate artificial antigen, a preparation method and an application thereof. The invention uses 2-aminoethanol hydrogen sulfate as a raw material to prepare an ethyl sulfate artificial antigen, and introduces 2-(4-(methyl bromide) into the amino group. base) phenyl) acetic acid linking arm to prepare an ethyl sulfate hapten, which reacts with N-hydroxysuccinimide and N,N-cyclohexylcarbodiimide to obtain an active ester, which is combined with The protein coupling reaction was carried out to obtain ethyl sulfate artificial antigen. The present invention only needs one step to obtain the hapten, the process is simple, the source of raw materials is not limited, and it also retains the characteristic structure of ETS, which has good specificity; the linker introduced by the present invention can maximize the potential of the antigen Immunogenicity, enhance the immune effect, stimulate animals to produce antibodies, and can effectively make up for the shortcomings of ETS, which is difficult to stimulate the body to produce antibodies because of its too simple structure.

Description

technical field [0001] The invention specifically relates to an ethyl sulfate artificial antigen, a preparation method and an application. Background technique [0002] Ethanol, commonly known as alcohol, is one of the most abused substances worldwide. Statistics show that drivers who drive after drinking are 15 times more likely to have an accident than usual, and 30% of road traffic accidents are caused by drinking and driving. 59% of driver fatalities on file are related to DUI. The shocking figures tell us that drunk driving seriously endangers traffic safety, harming others and harming the society. [0003] At present, traffic police usually use breath alcohol detectors to test drivers for alcohol. However, there are many problems with the breath alcohol tester, which affect the accuracy of the alcohol test results. Because there are many uncertain factors affecting the test results of the breath alcohol detector, for example, the breath alcohol detection technology...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C305/04C07C303/24C07K14/765C07K1/107C07K16/44G01N33/577G01N33/558G01N33/53
CPCC07C305/04C07K14/765C07K16/44G01N33/577G01N33/558G01N33/5308Y02P20/55
Inventor 王百龙王晨苏祥煌
Owner HANGZHOU BIOTEST BIOTECH CO LTD
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