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Application of urine stem cells in preparation of drug for improving glucose metabolism of mouse

A stem cell and glucose technology, applied in the medical field, can solve problems such as no practical application, and achieve the effect of prolonging the survival period, high efficiency, and improving glucose metabolism

Inactive Publication Date: 2017-05-17
SHANGHAI SIXTH PEOPLES HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above is only a theory, and there is no relevant practical application in the prior art

Method used

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  • Application of urine stem cells in preparation of drug for improving glucose metabolism of mouse
  • Application of urine stem cells in preparation of drug for improving glucose metabolism of mouse
  • Application of urine stem cells in preparation of drug for improving glucose metabolism of mouse

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1: Extraction and cultivation of human urine stem cells

[0020] Collect 150-200ml of urine into several 50ml sterile test tubes, centrifuge at room temperature at 400×g for 10min, carefully aspirate the supernatant, leave 1ml of urine to gently resuspend the precipitate, and then transfer it to a 50ml test tube , add 10ml of washing solution to the test tube, centrifuge at room temperature at 200×g for 10min, carefully discard the supernatant, leave 0.2ml of the supernatant, add 1ml of DMEM culture solution, and then transfer to a six-well plate coated with 0.1% gelatin, Add 2ml of DMEM culture solution to each well, culture at 37°C and 5% CO2, change the medium every 3 days to remove unattached cells, adherent cells are urine stem cells, and wait for the cells to reach about 80% fusion Passage later.

[0021] The mouse glucose metabolism-improving drug prepared by using the above-mentioned human urinary stem cells was orthotopically transplanted into the mous...

Embodiment 2

[0033] Example 2: Identification of urinary stem cell surface markers and pluripotent stemness

[0034] 1. Identification by Flow Cytometry

[0035] Proceed as follows:

[0036] (1) Digest the isolated and cultured third-generation urine stem cells and resuspend them with DMEM medium;

[0037] (2) Centrifuge at 1000rpm for 5 minutes, discard the supernatant and resuspend and wash with PBS buffer;

[0038] (3) Centrifuge again at 1000rpm for 5 minutes, discard the supernatant and resuspend the cells with PBS, and adjust the cell concentration to 1×10 by cell counting. 6 a / mL;

[0039] (4) Take each 200ul of the cell suspension and pack it into the sample tube;

[0040] (5) Add 5ul of different fluorescently labeled monoclonal antibodies (CD29-PE, CD45-PE, CD90-FITC, CD73-PE, CD146-PE and HLA-DR-PE) and mix well, and incubate at room temperature for 30 minute;

[0041] (6) Add 2ml of PBS buffer solution to each tube, mix well by pipetting;

[0042] Then centrifuge at 1000...

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Abstract

The invention provides an application of urine stem cells in preparation of a drug for improving glucose metabolism of a mouse. The urine stem cells are human urine stem cells. As a raw material for preparing the drug for improving glucose metabolism of the mouse, the urine stem cells have the advantages of being not limited by sources, low in cost, high in efficiency and the like. The drug which is prepared from urine stem cells and used for improving glucose metabolism of the mouse is transplanted to the mouse with abnormal glucose metabolism to remarkably improve the glucose metabolism of the mouse and prolong the survival period of the mouse and has practical value.

Description

technical field [0001] The invention belongs to the field of medicine, and in particular relates to the application of urine stem cells in the preparation of drugs for improving glucose metabolism in mice. Background technique [0002] Stem cells are a group of cells with high self-renewal and multi-directional differentiation potential, and are ideal cell sources for regenerative medicine and tissue function repair. Stem cell transplantation as a new substitute for islet transplantation provides a new idea for delaying or reversing diabetes. Traditional stem cell transplantation such as embryonic stem cell transplantation or adult stem cell transplantation often suffers from limited tissue sources, invasive sampling process, complicated differentiation process and high treatment costs, so it is difficult to promote and utilize on a large scale. Therefore, it is of great significance to develop a simple, non-invasive, safe and low-cost stem cell therapy. [0003] Urinary s...

Claims

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Application Information

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IPC IPC(8): A61K35/22A61P3/10
Inventor 王琛贾伟平汪泱牛鑫罗登孙赟赵天雪
Owner SHANGHAI SIXTH PEOPLES HOSPITAL
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