Kit for extracting blood genome DNA based on magnetic bead method and use method for kit
A technology of genome and magnetic bead method, which is applied in the field of molecular biology, can solve the problems of increased workload of experimenters and increased extraction time, and achieve the effects of busy procedures, improved work efficiency, and high DNA extraction yield
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0034] A kit for extracting blood genomic DNA based on the magnetic bead method, comprising independently packaged:
[0035] Cell lysate: a mixed solution of 6M guanidine hydrochloride, 0.5M sodium chloride and 1% Tween 20 by volume fraction. Specific preparation method: Weigh 573g of guanidine hydrochloride and 29g of sodium chloride, add 800mL of deionized water and stir to dissolve, add 10mL of Tween 20, continue to add deionized water to make up to 1L.
[0036] Proteinase K solution: Proteinase K solution is a mature commercial product, which is a serine protease with broad cleavage activity, purified from Candida albicans Limberella. The proteinase K solution is a solution obtained by dissolving proteinase K in water.
[0037] Magnetic bead dispersion: magnetic bead suspension with a concentration of 20mg / mL, the core of the magnetic bead is ferric oxide (Fe 3 o 4 ), the surface is coated with SiO 2 layer. Specific preparation method: Weigh 2g of magnetic beads, add ...
Embodiment 2
[0055] Use the kit of Example 1 to carry out high-throughput extraction experiments on 16 parts of blood genomic DNA:
[0056] Step 1: Thaw the frozen blood sample at 4°C or at room temperature; stabilize the water bath to 58°C; set the vibration frequency of the vortex mixer to 1150rpm.
[0057] Step 2: Add 200 μL of blood sample, 10 μL of proteinase K solution, and 200 μL of cell lysate to the 48-well plate in sequence with a multi-channel automatic pipette, 16 samples in parallel, vortex for 30 seconds to mix well, and place in a water bath Lyse for 10 minutes.
[0058] Step 3: Take out the 48-well plate, add 100 μL of magnetic bead dispersion and 300 μL of isopropanol to the 16 sample wells with a multi-channel automatic pipette, place the 48-well plate in a vortex mixer for 5 minutes, and place Place the 48-well plate on the 48-well plate magnetic plate rack and let it stand for 20 seconds until the magnetic beads are completely adsorbed. Keep the 48-well plate fixed on ...
PUM
Property | Measurement | Unit |
---|---|---|
particle diameter | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com