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Method for preparing leucomalachite green immunogen

A colorless malachite green immunogen technology, applied in peptide preparation methods, chemical instruments and methods, animal/human proteins, etc., can solve the problem of prolonging the process of synthesizing colorless malachite green immunogen, increasing product costs, and using Problems such as cost increase, to achieve the effect of popularization, promotion and application, improvement of production efficiency and efficiency improvement

Inactive Publication Date: 2011-10-05
SHANGHAI OCEAN UNIV
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Problems solved by technology

[0002] The preparation of the colorless malachite green immunogen usually involves the process of synthesizing the colorless malachite green immunogen. However, in the process of synthesizing the colorless malachite green immunogen, the existing methods such as the diazotization method are generally divided into two steps It takes about 70 hours to carry out the coupling synthesis, which will prolong the process of synthesizing the colorless malachite green immunogen, resulting in a decrease in production efficiency and an increase in product cost. At the same time, the cost of subsequent use is also increased. In addition, The coupling ratio is generally only about 2-10

Method used

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Embodiment Construction

[0009] The preparation method of a colorless malachite green immunogen of the present invention will be further described in detail below.

[0010] Specific steps are as follows:

[0011] 1. Synthetic amino colorless malachite green

[0012] a) Weigh 0.2g of colorless malachite green and dissolve it in 2mL of concentrated sulfuric acid, stir and heat to maintain the temperature at 90°C, add dropwise a mixture of 2mL of concentrated sulfuric acid and 2mL of concentrated nitric acid, react for 2 hours, and add the reaction solution to 3-10mL of ice water after cooling , add sodium bicarbonate dropwise to neutralize to alkaline (pH 7.5-10), and yellow crystals precipitate out;

[0013] b) Add 10mL of hydrochloric acid solution to the obtained yellow crystals, then add 1g of zinc powder, react at 85°C-95°C oil bath temperature for 5 hours, add 15mL of distilled water after the reaction is completely cooled, stir and filter to remove solid impurities, and use hydrogen to filter th...

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Abstract

The invention discloses a method for preparing leucomalachite green immunogen, which is characterized by comprising: adding bovine serum albumin, leucomalachite green immunogen, 1-[3-(dimethylamino)propyl]-3-ethylcarbodiimide hydrochloride into phosphate buffer solution one by one according to a molar ratio of 1:592:5216; reacting for 2 hours at room temperature; filling liquid obtained after reaction into a dialysis bag; filling into 500 milliliters of distilled water, dialyzing for 48 hours, and changing liquid once every 12 hours; and collecting liquid in the dialysis bag, pre-freezing at -20 DEG C for 2 hours, freezing at -50 DEG C, and drying to obtain leucomalachite green immunogen purple solid powder. In the invention, the preparation process is simplified, the time for preparing the leucomalachite green immunogen is shortened, the production efficiency is improved by about 49 percent, the production cost is reduced, and the promotion and use are easy.

Description

technical field [0001] The invention relates to a colorless malachite green immunogen, in particular to a preparation method of the colorless malachite green immunogen. Background technique [0002] The preparation of the colorless malachite green immunogen usually involves the process of synthesizing the colorless malachite green immunogen. However, in the process of synthesizing the colorless malachite green immunogen, the existing methods such as the diazotization method are generally divided into two steps It takes about 70 hours to carry out the coupling synthesis, which will prolong the process of synthesizing the colorless malachite green immunogen, resulting in a decrease in production efficiency and an increase in product cost. At the same time, the cost of subsequent use is also increased. In addition, The coupling ratio is generally only about 2-10. Contents of the invention [0003] The object of the present invention is to provide a preparation method of the ...

Claims

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Application Information

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IPC IPC(8): C07K14/765C07K1/113
Inventor 陈力杨先乐胡鲲姜有声孙琪
Owner SHANGHAI OCEAN UNIV
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