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Preparation method and application of leucomalachite green standard sample in snakehead meat powder

A recessive malachite green, standard sample technology, applied in the field of analytical chemistry, can solve problems such as the state that cannot represent the existence of the target analyte, the state that cannot represent the internalization of the target analyte, and the reliability and validity of the results. Achieve significant economic value and market competitiveness, uniform and stable samples, and convenient transportation.

Pending Publication Date: 2021-10-19
四川省食品药品检验检测院 +3
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the standard substance used for residual detection of recessive malachite green is mainly pure product recessive malachite green standard sample, and the detection quality control is mainly to carry out detection and analysis after adding pure recessive malachite green standard substance to the negative sample, This analytical method cannot represent the true state of the target analyte in vivo, that is, it cannot represent the "internalized" state of the target analyte
The standard sample obtained in this way is not a real physical standard sample. Because the combination of the target object and the matrix is ​​not completely consistent with the real test sample, the results of extraction, purification, etc. are different from the daily analysis samples, which affects the reliability of the results. sex and effectiveness

Method used

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  • Preparation method and application of leucomalachite green standard sample in snakehead meat powder
  • Preparation method and application of leucomalachite green standard sample in snakehead meat powder
  • Preparation method and application of leucomalachite green standard sample in snakehead meat powder

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1 Experimental method

[0026] For the experimental methods that do not indicate specific conditions in the following experimental methods, usually follow the conventional conditions or the conditions suggested by the manufacturer.

[0027] The specific steps are as follows:

[0028] (1) Selection of experimental animals: buy commercially available black fish, first confirm that the black fish does not contain malachite green and recessive malachite green, take random samples, and feed them after the test is confirmed to be negative. Negative snakeheaded fish were fed to a stable state (about 2 days) in water without malachite green and recessive malachite green at 20°C -25°C, and the injured and unhealthy snakeheaded fish were removed;

[0029] (2) Medicated bath feeding: use 0.1mg / L recessive malachite green standard solution for 96 hours in medicated bath, and then replace with clear water for 24 hours of metabolism after the medicated bath, so that the d...

Embodiment 2

[0036] Embodiment 2 Experimental results

[0037] 1. Uniformity test

[0038] The homogeneity test was carried out by using the one-way analysis of variance method, and 12 bottles of samples prepared in Example 1 were randomly selected, and each bag of samples was measured twice in parallel. All samples are tested in random order under repetitive conditions, that is, in the same laboratory by the same personnel using the same test methods and instruments for a short period of time.

[0039] Test method: Standard sample weighing 1.0 g at one time, add 4.0 mL of distilled water to restore, which is equivalent to 5.0 g of wet sample, follow-up operations refer to "GB / T 19857-2005 Determination of Malachite Green and Crystal Violet Residues in Aquatic Products 2 Liquid chromatography-tandem mass spectrometry" is detected, and the results are shown in Table 1, Table 2, Table 3 and Table 4.

[0040]

[0041]

[0042] F critical value F 0.05(11，12) =2.72. computational F...

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Abstract

The invention discloses a preparation method of a leucomalachite green standard sample in snakehead meat powder. The preparation method comprises the steps of selecting an experimental animal, carrying out medicine feeding on fresh and live snakeheads in a malachite green medicated bath mode, controlling the medicated bath time, and carrying out animal metabolism to obtain a sample material containing leucomalachite green; adding an antioxidant and a preservative into fresh meat, uniformly stirring, freeze-drying, crushing, sieving, adjusting the concentration, uniformly mixing, sub-packaging, and irradiating to prepare the leucomalachite green standard sample in the snakehead meat powder. The standard sample obtained by the invention can be transported at normal temperature, has long shelf life, can be used for quality control of related detection analysis, is uniform and stable, is convenient to transport and reliable to use, and has remarkable economic value and market competitiveness, and the binding situation of a target object and a matrix is consistent with that of a real detection sample.

Description

technical field [0001] The invention belongs to the field of analytical chemistry, and in particular relates to a method for preparing a standard sample of recessive malachite green in black fish meal. Background technique [0002] Malachite green (Malachite green, MG), also known as basic green, base block green, malachite green, is a dark green crystalline solid, easily soluble in water, and belongs to triphenylmethane chemical substances. It has special effects against saprolegniasis in fish and fish eggs. Currently, there is no specific drug against saprolegniasis in the market that can solve saprolegniasis in a short time. The use of malachite green in aquaculture has been banned repeatedly. Malachite green is metabolized into colorless leucomalachite green (LMG) in aquatic animals. Malachite green and colorless leucomalachite green tend to remain in aquatic animals for a long time, resulting in highly teratogenic, carcinogenic and Mutagenic effect, recessive malachite...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28G01N1/42G01N1/38
CPCG01N1/28G01N1/286G01N1/42G01N1/38G01N2001/2893
Inventor 成长玉李澍才李道霞余晓琴魏丹丹龚利新
Owner 四川省食品药品检验检测院
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