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Leucomalachite green detection method

A colorless malachite green and detection method technology, applied in the direction of material excitation analysis, fluorescence/phosphorescence, etc., can solve the problems of low sensitivity, expensive instruments, low detection sensitivity, etc., and achieve high application value, easy access to reagents, and high sensitivity Effect

Inactive Publication Date: 2011-01-19
KUNMING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, some detection methods have appeared again. The relevant patent documents include the rapid detection method of malachite green and colorless malachite green residues in water bodies and aquatic products and the detection box used in this method (patent application number 200810025045.9). It involves a semi-quantitative method with low detection sensitivity, while other detection methods involving colorless malachite green are enzyme-linked immunosorbent methods
Some of these detection methods are complex, expensive instruments, high pre-treatment requirements, and time-consuming; some are easy to operate, but have low sensitivity and errors in measurement

Method used

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  • Leucomalachite green detection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] (1) Use a micro sampler to pipette 0.1mL of HAc-NaAc buffer solution with a pH value of 3.5 into a 10mL test tube, and add 1.5mL to the solution in the test tube successively with a concentration of 1.0×10 -3 mol / L of I 3 - Solution and 0.1mL of colorless malachite green with known different concentrations, shake well, then add 0.1mL of cocamidopropyl betaine (CAB-35) with a concentration of 10mg / L, dilute to 10mL with acetonitrile, and keep away from light Leave it for 5 minutes to obtain the tested system;

[0031] (2) Prepare a reagent blank system according to the method of step (1), without adding colorless malachite green;

[0032] (3) The above-mentioned measurement system made above is equal to the emission wavelength with a fluorescence spectrophotometer, and the excitation wavelength is equal to the emission wavelength, and it is scanned synchronously in the range of 400 to 600 nm to obtain the system resonance Rayleigh scattering spectrum, and measure the l...

Embodiment 2

[0037] (1) Use a micro sampler to pipette 1.0mL of HAc-NaAc buffer solution with a pH value of 4.0 into a 10mL test tube, and add 1.5mL to the solution in the test tube successively with a concentration of 1.0×10 -3 mol / L ClO 3 - Solution and 0.1mL of colorless malachite green with known different concentrations, shake well, then add 0.5mL of cocoyl dimethyl betaine (BS-12) with a concentration of 10mg / L, dilute to 10mL with acetonitrile, avoid Leave it alone for 10 minutes to obtain the system to be tested;

[0038] (2) Prepare a reagent blank system according to the method of step (1), without adding colorless malachite green;

[0039] (3) The above-mentioned measurement system made above is equal to the emission wavelength with a fluorescence spectrophotometer, and the excitation wavelength is equal to the emission wavelength, and the synchronous scanning in the range of 400~600nm obtains the system resonance Rayleigh scattering spectrum, selects the light scattering inte...

Embodiment 3

[0044] (1) Use a micro sampler to pipette 1.5mL of HAc-NaAc buffer solution with a pH value of 4.5 into a 10mL test tube, and add 0.5mL of the solution in the test tube to a concentration of 1.0×10 -3 mol / LPO 4 3- solution and 0.1 mL of colorless malachite green with known different concentrations, shake well, then add 1.0 mL of lauryl amidopropyl betaine (LAB-30) with a concentration of 10 mg / L, dilute to 10 mL with acetonitrile, and place in the dark 15min, the system to be tested was obtained;

[0045] (2) Prepare a reagent blank system according to the method of step (1), without adding colorless malachite green;

[0046] (3) With the above-mentioned measurement system made by using a fluorescence spectrophotometer, equal to the emission wavelength with the excitation wavelength, synchronous scanning in the range of 400 ~ 600nm, to obtain the system resonance Rayleigh scattering spectrum, select the light scattering intensity I of the measurement system at the 494nm wave...

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Abstract

The invention discloses a method for detecting leucomalachite green in an aquatic product, which comprises the following steps: adding anions and leucomalachite green to a weakly acidic HAc-NaAc buffer solution, shaking up, adding amphoteric surface active agent, maintaining a constant volume, standing in a dark place for some time to obtain a concentration-known detected system, carrying out synchronous scanning in a range of 400-600 nm by utilizing a fluorophotometer under the condition that the excitation wavelength is equal to the emission wavelength to obtain the light scattering strength I and the reagent blank I0 of the system, calculating according to the formula of deltaI=I-I0 to obtain the deltaII value of the object to be detected, and calculating the leucomalachite green content of a certain unknown object to be detected according to working curves. The method has the advantages of low price and easy acquisition of reagent, moderate reaction condition, simple operation, simple equipment, high sensitivity and easy popularization.

Description

technical field [0001] The invention relates to a detection method for colorless malachite green, in particular to a resonance Rayleigh scattering method for detecting colorless malachite green. Background technique [0002] Malachite green (Malachite green, MG), chemical name is tetramethyl-substituted diaminotriphenylmethane, molecular formula C 23 h 25 ClN 2 , which belongs to triphenylmethane dyes, is easily soluble in water, ethanol, methanol, and acetonitrile, and the solution is blue-green. It is a biological dye and is also used as an antibacterial agent and preservative for aquatic animals. Colorless malachite green (Leμcomalachite green, LMG), chemical name is N, N-dimethylaniline, molecular formula C 23 h 26 N 2 , which is the reduced metabolite of malachite green in aquatic animals. According to scientific research, both malachite green and colorless malachite green have high toxicity, high residue, high carcinogenicity, high teratogenicity and mutagenic ha...

Claims

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Application Information

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IPC IPC(8): G01N21/64
Inventor 杨亚玲邹艳李冉
Owner KUNMING UNIV OF SCI & TECH
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